Research Article

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DNA fingerprinting; Genetic diversity; Olea europaea; RAPD

A total of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura region of central-western Spain were analyzed using morphological traits and RAPD markers. We focused on three main and 16 minor Spanish olive cultivars that are recognized as native or local to the Extremadura region. The five arbitrary 10-mer primers tested on the olive cultivars gave 67 polymorphic ... more

M.C. Parra-Lobato; F.J. Delgado-Martinez; M.C. Gomez-Jimenez
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Genetic diversity; Microsatellite; Molecular breeding; Popcorn; SSR locus; Zea mays

We analyzed genetic structure and diversity among eight populations of popcorn, using SSR loci as genetic markers. Our objectives were to select SSR loci that could be used to estimate genetic diversity within popcorn populations, and to analyze the genetic structure of promising populations with high levels of heterozygosity that could be used in breeding programs. Fifty-seven alleles (3 ... more

I.B.O. Eloi; C.A. Mangolin; C.A. Scapim; C.S. Gonçalves; M.F.P.S. Machado
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Carica papaya; Genetic diversity; Gower algorithm; Molecular markers

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Planting resistant varieties is the most effective control measure against the angular leaf spot of dry beans, a fungal disease caused by Pseudocercospora griseola. However, dry bean varieties with durable resistance are not easily obtained. Knowledge about the genetic variability of the pathogen population is key for the success of dry bean breeding programs aimed at developing ... more

A.K.R. Abadio; S.S. Lima; M.F. Santana; T.M.F. Salomão; A. Sartorato; E.S.G. Mizubuti; E.F. Araújo; M.V. de Queiroz
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Genetic diversity; Grape phylloxera; Population genetics; Simple sequence repeat marker

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Barley landraces; Genetic diversity; Hordeum vulgare; PIC; SSR markers

We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixty-six alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, with a mean of 0.732, indicating ... more

Z.W. Chen; R.J. Lu; L. Zou; Z.Z. Du; R.H. Gao; T. He; J.H. Huang
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Genetic diversity; Jatropha curcas L.; Methylation-sensitive amplification polymorphism

Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (AFLP) markers, 471 markers were scored. All 56 samples ... more

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DNA fingerprinting; Fruit-endocarp or fruit-pit characters; Genetic diversity; Olea europaea; SSR

Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 ... more

F.J. Delgado-Martinez; I. Amaya; J.F. Sánchez-Sevilla; M.C. Gomez-Jimenez

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