Research Article

Related GMR Articles

12/12/2002
Aspergillus nidulans; ATP-binding cassette transporters; Fungal infections; Multidrug resistance

As a preliminary step towards characterizing genes encoding ATP-binding cassette (ABC) transporters that confer pleiotropic drug resistance in Aspergillus, we used a PCR-based approach to isolate four DNA fragments corresponding to different ABC type transporter genes. DNA sequencing and Southern blot analysis confirmed that they were distinct genes, which were designated ... more

A.Mendes do Nascimento; M.Helena S. Goldman; G.H. Goldman
03/30/2003
Antimicrobials; Drug tolerance; Efflux pumps; Multidrug resistance; Pseudomonas

Pseudomonas aeruginosa is an opportunistic human pathogen exhibiting innate resistance to multiple antimicrobial agents. This intrinsic multidrug resistance is caused by synergy between a low-permeability outer membrane and expression of a number of broadly-specific multidrug efflux (Mex) systems, including MexAB-OprM and MexXY-OprM. In addition to this ... more

H.P. Schweizer
12/09/2008
Multidrug resistance; multidrug resistance protein; Single nucleotide polymorphism

One of the main problems in treating cancer patients is that cancer cells can develop drug resistance. Resistance to multiple anticancer drugs, so called multidrug resistance (MDR), most likely involves a nonspecific mode of resistance, through drug-efflux transporters. One of the most extensively studied genes involved in MDR is multidrug resistance protein 1 (MRP1). We investigated ... more

F. Mahjoubi; S. Akbari; M. Montazeri; F. Moshyri
01/12/2010
Breast cancer; C3435T polymorphism; MDR1 gene; Multidrug resistance; Real-time polymerase chain reaction

One of the limitations in the treatment of cancer patients with chemotherapy is the development of multidrug resistance (MDR). A well-known mechanism responsible for drug resistance is over-expression of ABC-transporter genes such as MDR1. This gene encodes p-glycoprotein (P-gp), a transmembrane glycoprotein that transports many hydrophobic substrates and anti-cancer drugs out of the cell ... more

M. Taheri; F. Mahjoubi; R. Omranipour
02/27/2014
Apoptosis-related protein; Glucosylceramide synthase gene; Mifepristone; Multidrug resistance

We examined the ability of mifepristone to reverse the in vitro drug resistance of human cervical cancer cells resistant to mitomycin-C (HeLa/MMC) cells and investigated the mechanism of this effect. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to detect the drug resistance of HeLa/MMC cells and the reversed drug resistance in vitro ... more

H. Chen; J. Duan; F. Zuo
04/13/2015
Cancer stem cells; Chemotherapy; Medulloblastoma; Multidrug resistance; Side population

Brain cancer stem cells are a subset of tumor cells present in several types of brain tumor that evade treatment regimens and are responsible for tumor recurrence. Recent reports on several tumors have suggested that Hoechst 33342 dye exclusion is a powerful technique for isolating cancer stem cell-like side population (SP) cells. In the present study, we attempted to isolate the SP cells ... more

J. Liu; N. Chi; J.Y. Zhang; W. Zhu; Y.S. Bian; H.G. Chen
06/17/2016
Fibroblast-like synoviocytes; Multidrug resistance; P-glycoprotein; Refractory rheumatoid arthritis

This study aims to investigate the role of P-glycoprotein (P-gp) expression level in drug resistance to disease-modifying anti-rheumatic drugs in refractory rheumatoid arthritis (RRA). We evaluated and compared the expression levels of P-gp in fibroblast-like synoviocyte (FLS) cells in patients with rheumatoid arthritis (RA) and osteoarthritis (OA), and investigated the potential ... more

Y.M. Liu; J.W. Chen; L.X. Chen; X. Xie; N. Mao; Y.M. Liu; J.W. Chen; L.X. Chen; X. Xie; N. Mao
10/30/2015
Infant; p-JAK1/p-STAT6/SOCS5; Vitamin D; Weaning rats

Asthma is a chronic inflammatory disease of the airways with variable airflow obstruction and bronchial hyper-responsiveness. It is believed that Th2-derived cytokines orchestrate the asthmatic response and the maintenance of the balance of Th1/Th2 plays a crucial role in prevention of asthma. Moreover, 1,25(OH)2D3, the biologically activate form of vitamin D, was reported with ... more

X. Chen; S.Q. Rao; B.H. Gao; Z.Q. Jiang
05/25/2017
Cell line; Cell proliferation; Epidermal growth factor; Humans; Ki-67 Antigen; Mesangial cells; Vitamin D; Vitamins

Previous studies have found that 1,25-dihydroxyvitamin D [1,25(OH)D or VD] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)D on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and ... more

D. Zhao; C.J. Zhang; R. Yang; J.P. Chen; L. Ma; G. Liu; X.P. Yang