Research Article

Related GMR Articles

02/02/2010
Filamentous fungi; Fusarium oxysporum; Genomic DNA extraction; Polymerase chain reaction; Pyrenochaeta terrestris

DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terrestris, which causes pink root in onions. This ... more

D. González-Mendoza; R. Argumedo-Delira; A. Morales-Trejo; A. Pulido-Herrera; L. Cervantes-Díaz; O. Grimaldo-Juarez; A. Alarcón
05/15/2012
Filamentous fungi; Genomic DNA extraction; Trichoderma

Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, without maceration in liquid nitrogen and without a final ethanol ... more

J.C. Vazquez-Angulo; V. Mendez-Trujillo; D. González-Mendoza; A. Morales-Trejo; O. Grimaldo-Juarez; L. Cervantes-Díaz
10/26/2006
Ganga; Hooghly; Narmada; PCR; RAPD; Tenualosa ilisha; Yamuna

RAPD was used to delineate the hilsa populations sampled from the Ganga, Yamuna, Hooghly, and Narmada Rivers at six different locations. Six degenerate primers were used to generate the fragment patterns from the samples collected. All primers were highly polymorphic and generated high numbers of amplification products. Nei’s genetic distances were calculated between locations. The ... more

M.P. Brahmane; M.K. Das; M.R. Sinha; V.V. Sugunan; A. Mukherjee; S.N. Singh; S. Prakash; P. Maurye; A. Hajra
12/08/2011
Amplification success; Barcoding; Birds; Cytochrome oxidase 1; Mammals; Mini-barcode; PCR; Reptiles

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single ... more

I.A. Arif; H.A. Khan; A. Sadoon; M. Shobrak
10/27/2011
BLV testing; Bovine leukemia virus; Cattle; Leukosis; Native Iranian and Russian breeds; PCR

Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

M.R. Mohammadabadi; M. Soflaei; H. Mostafavi; M. Honarmand
10/19/2011
12S rRNA; Bos gaurus; COI; Cyt b; Malayan gaur; PCR

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine ... more

M.K.A. Rosli; A.S. Zamzuriada; S.M.F. Syed-Shabthar; M.C. Mahani; O. Abas-Mazni; B.M. Md-Zain
08/05/2011
Cereal seeds; DNA extraction; PCR

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves ... more

Z.H. Pervaiz; N.A. Turi; I. Khaliq; M.A. Rabbani; S.A. Malik
07/06/2011
Endometriosis; PCR; Progesterone receptor; PROGINS

We investigated a possible link between endometriosis and polymorphism of the progesterone receptor gene (PROGINS). The endometriosis group consisted of 54 patients with a diagnosis of endometriosis by laparoscopy, and the control group comprised 44 women without endometriosis. Genotypes for PROGINS polymorphisms (A1/A1, A1/A2 and A2/A2) were determined by polymerase chain reaction and ... more

I.R. Costa; R.C.P.C. Silva; A.B. Frare; C.T.X. Silva; B.M. Bordin; S.R. Souza; C.L.Ribeiro Júnior; K.K.V.O. Moura
06/28/2011
Corynebacterium diphtheriae; GenoFrag; Genome plasticity; Horizontal gene transfer; Pathogenicity islands; PCR

Despite the existence of a vaccine against diphtheria, this disease remains endemic and is reemerging in several regions due to many factors, including variations in genes coding for virulence factors. One common feature of virulence factors is their high concentration in pathogenicity islands (PAIs), very unstable regions acquired via horizontal gene transfer, which has lead to the ... more

S.C. Soares; F.A. Dorella; L.G.C. Pacheco; R. Hirata; A.L. Mattos-Guaraldi; V. Azevedo; A. Miyoshi
03/29/2011
DNA extraction; PCR; Protocol

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single ... more

T.Y. Wang; L. Wang; J.H. Zhang; W.H. Dong

Pages