Research Article

Related GMR Articles

Echinococcus granulosus; Heteroduplex DNA; Microsatellite markers; U1 snRNA gene

Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobility in polyacrylamide gel electrophoresis. Heteroduplex ... more

P.A. Roratto; M.L. Bartholomei-Santos; A.M. Gutierrez; L. Kamenetzky; M.C. Rosenzvit; A. Zaha
Meat traceability; Microsatellite markers; Synthetic breeds

Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality ... more

R. Rodríguez-Ramírez; A. Arana; L. Alfonso; A.F. González-Córdova; G. Torrescano; G. Legarreta; B. Vallejo-Cordoba
Association study; Carcass traits; Cattle; Microsatellite markers

Investigation of molecular marker effects on production traits is essential to define marker assisted selection strategies in beef cattle. We looked for a possible association of molecular markers and backfat thickness (BFT) and rib eye area (REA) in Canchim (5/8 Charolais + 3/8 Zebu) and MA (offspring of Charolais bulls and 1/2 Canchim + 1/2 Zebu cows) animals raised exclusively on ... more

S.L. Meirelles; G.V. Gouveia; G. Gasparin; M.M. Alencar; J.J.S. Gouveia; L.C.A. Regitano
5' Anchored PCR; Larimichthys polyactis; Microsatellite markers; Polymorphism; Redlip croaker

Larimichthys polyactis is a commercially important marine fish species in southeast Asia. The population crashed due to overfishing in the 1970s, but has since recovered. We developed 13 novel polymorphic microsatellite markers in L. polyactis using 5' anchored PCR. The characteristics of these loci were estimated by analyzing a sample of 30 individuals. A total of 74 ... more

C.Y. Ma; H.Y. Ma; L.B. Ma
Backcross; Genetic variability; Inbreeding; Microsatellite markers; Parental genome

The limited number of papaya varieties available reflects the narrow genetic base of this species. The use of backcrossing as a breeding strategy can promote increases in variability, besides allowing targeted improvements. Procedures that combine the use of molecular markers and backcrossing permit a reduction of the time required for introgression of genes of interest and appropriate ... more

H.C.C. Ramos; M.G. Pereira; F.F. Silva; L.S.A. Gonçalves; F.O. Pinto; G.Ade Souza Filho; T.S.N. Pereira
Beetle; Brontispa longissima; Microsatellite markers; Polymorphism

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of ... more

C.L. Ma; H.L. Wu; H.Y. Hu; X. Wu; G.C. Ma; Y.G. Fu; Z.Q. Peng
Chicken; Cross-species amplification; Grey partridge; Microsatellite markers; Turkey

We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of ... more

N. Bech; C. Novoa; J.F. Allienne; J. Boissier
Cross-amplification; Microsatellite markers; Nyctereutes procyonoides procyonoides

Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. Information about the genetic background of farmed Chinese raccoon dogs is limited. In this study, 17 polymorphic microsatellite markers were isolated and identified from an (AC)n-microsatellite-enriched library of Chinese raccoon dogs. The number of ... more

S.Q. Yan; Y.M. Li; C.Y. Bai; X.M. Ding; W.J. Li; J.N. Hou; Z.H. Zhao; J.H. Sun
African Silverbil; Cross-species amplification; Lonchura cantans; Microsatellite markers

We tested the cross-amplification of eight microsatellites developed for Bengalese finch in African Silverbill (Lonchura cantans). In order to develop resources for conservation genetic studies in the species L. cantans, we tested the amplification success and polymorphism in eight previously developed microsatellite loci, in L. cantans. All eight microsatellite ... more

N.R. Parine; D. Kumar; A.A.K. Pathan; M.S. Elrobh; W. Khan; M. Alanazi
American cattle breeds; Brazilian cattle; Genetic diversity; Microsatellite markers; Population structure

The Brazilian cattle population is mainly composed of breeds of zebuine origin and their American derivatives. Comprehensive knowledge about the genetic diversity of these populations is fundamental for animal breeding programs and the conservation of genetic resources. This study aimed to assess the phylogenetic relationships, levels of genetic diversity, and patterns of taurine/zebuine ... more

B.S.A.F. Brasil; E.G.A. Coelho; M.G. Drummond; D.A.A. Oliveira