Research Article

Related GMR Articles

Bats; Chiroptera; Fluorescent in situ hybridization; Genetic variation; Random amplified polymorphic DNA

Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by ... more

P.R.L. Moreira; E. Morielle-Versute
Ananas comosus var. bracteatus; Genetic variation; In vitro culture; Mutation; Somaclonal variation

The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/L. The addition of BAP influenced the ... more

M.D.M. Santos; G.C.S. Buso; A.C. Torres
Association analysis; Genetic variation; Goat; Production traits; Sine oculis homeobox homolog 6 (Six6) gene

We looked for novel genetic variations within the Six6 gene by PCR-SSCP, DNA sequencing and forced RFLP-PCR and estimated their associations with production traits in 2132 goats of eight indigenous Chinese breeds. A novel single nucleotide polymorphism (NM_001104993.1: g.232T>C) within the goat Six6 gene was identified. The frequencies of allele “C” varied from 0.8621 ... more

C.Y. Pan; X.Y. Lan; H.Y. Zhao; S.R. Hu; Y.T. Huai; C.Z. Lei; H. Chen
Genetic variation; Morphometric; Multivariate analysis; RAPD; Rastrelliger spp

Mackerel (Scombridae; Rastrelliger) are small commercially important pelagic fish found in tropical regions. They serve as a cheap source of animal protein and are commonly used as live bait. By using a truss morphometrics protocol and RAPD analysis, we examined morphological and genetic variation among 77 individual mackerel that were caught using long lines and gillnets at 11 ... more

M.N. Darlina; A.R. Masazurah; P. Jayasankar; A.F.J. Jamsari; A.M.N. Siti
Efficacy; Genetic variation; Novel bands; Primer-pair arrays; RAPD

The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification ... more

E.M. Gatphoh; S.K. Sharma; K. Rajkumari; R. Rao
Conservation; Genetic variation; Microsatellites; RAMs; Scleropages formosus

Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The number of alleles at the 10 microsatellite loci ranged ... more

B. Manoharan; Z. Sulaimen; F. Omar; R.Y. Othman; S.Z. Mohamed; S. Bhassu
Drought tolerance; Genetic variation; Linkage maps; Molecular markers; Quantitative trait loci; Zea mays

Drought tolerance is one of the most important but complex traits of crops. We looked for quantitative trait loci (QTLs) that affect drought tolerance in maize. Two maize inbreds and their advanced lines were evaluated for drought-related traits. A genetic linkage map developed using RFLP markers was used to identify QTLs associated with drought-related traits. Twenty-two QTLs were ... more

H. Rahman; S. Pekic; V. Lazic-Jancic; S.A. Quarrie; S.M.A. Shah; A. Pervez; M.M. Shah
Genetic variation; Maize; Quantitative traits; RAPD markers; Zea mays

We compared DNA-based genetic diversity estimates with conventional estimates by investigating agronomically important traits in maize grown in the northwestern region of Pakistan. RAPD markers were used to characterize 10 commonly cultivated maize genotypes. The same material was tested for phenotypic variation of quantitative traits using replicated field trials. The genetic distances ... more

M.M. Shah; S.W. Hassan; K. Maqbool; I. Shahzadi; A. Pervez
Apolipoprotein B; Child; Genetic variation; Hyperlipidemia; Nephrotic syndrome

Apolipoprotein B (apoB) gene 3' variable number of tandem repeat (VNTR) is highly variable, and therefore can be an informative marker for associative analysis of lipid metabolism. This is the first report focusing on a possible association of apoB VNTR polymorphism with nephrotic hyperlipidemia. Genomic DNA was extracted from 500 children with primary nephrotic syndrome (PNS) and 500 ... more

P. Hu; B. Hu; Y.H. Qin; L. Lu; Z.Q. Li; L.Q. Tao; Q. Pei; J. Chen
Agronomic traits; Genetic variation; Gossypium hirsutum L.; ISSR; RAPD; SSR

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers ... more

Z. Noormohammadi; H.A. Farahani; M. Sheidai; S. Ghasemzadeh-Baraki; O. Alishah