Research Article

Related GMR Articles

05/22/2015
Actinomycetes; Antifungal activity; Antimicrobial activity; nrps gene; pks gene; Streptomyces ansochromogenes

The increased incidence of fungal infections and the development of drug resistance have led to the search for microorganisms capable of producing bioactive metabolites with antifungal activity. Among these microorganisms, Streptomyces spp are distinguished mainly owing to their potential to secrete bioactive molecules. The aim of this study was to evaluate the production of ... more

N.M. Vasconcelos; J.M. Fontes; M.R.C.R. Lins; G.R.B. Bernardo; J.M. Araújo; G.M.S. Lima
11/24/2009
Endophytic bacteria; Erwinia; Pantoea; Plant-bacteria interaction

Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E. grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified rDNA restriction analysis (ARDRA). ... more

R.E.L. Procópio; W.L. Araújo; W. Maccheroni; J.L. Azevedo
10/15/2013
16S rRNA; Endophytic bacteria; Microbial diversity; Sugarcane

Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified ... more

G.S. Magnani; L.M. Cruz; H. Weber; J.C. Bespalhok; E. Daros; V. Baura; M.G. Yates; R.A. Monteiro; H. Faoro; F.O. Pedrosa; E.M. Souza
04/03/2014
16S rDNA; Caragana microphylla; Diversity; Endophytic bacteria

The diversity of endophytic bacteria in the sand-fixation plant Caragana microphylla was investigated by amplified rDNA restriction analysis and by sequence and phylogenetic comparisons of the 16S rRNA genes. A total of 24, 19, and 17 operational taxonomic units were identified from 16S rDNA libraries of the plant roots, stems and leaves, respectively. Homology analysis revealed a ... more

J.X. Dai; X.M. Liu; Y.J. Wang
07/04/2014
Antifungal activity; Genetic diversity; Hemolysis; Medicago truncatula; Rhizosphere

In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10-2). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding ... more

J.E. Hernandez-Salmeron; C.M. Prieto-Barajas; E. Valencia-Cantero; G. Moreno-Hagelsieb; G. Santoyo
06/10/2015
Antifungal activity; Clerodendrum inerme; Endophyte; Latent pathogen

Fungal endophytes live in the inner tissues of Clerodendrum inerme and may be significant resources for new chemicals in drug discovery. A total of 242 endophytic fungi were recovered from 602 sample segments of C. inerme; 66 were purified. The 66 fungi belonging to 16 taxa and 11 genera (Alternaria, Nigrospora, Bartalinia, Pestalotiopsis ... more

B. Gong; X.H. Yao; Y.Q. Zhang; H.Y. Fang; T.C. Pang; Q.L. Dong
12/01/2015
3-1; 4-glucanase; Antifungal activity; Bacillus subtilis; Heterologous expression; Sclerotinia sclerotiorum; β-1

An antifungal protein exhibiting a high activity against Sclero­tinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4-glucanase according to amino acid analysis, and showed ... more

N.N. Wang; X.N. Gao; X. Yan; Z.P. Li; Z.S. Kang; L.L. Huang; Q.M. Han
05/31/2020
Genetic resources; Ginger; ISSR; Molecular characterization

Ginger (Zingiber officinale Roscoe) is a self-incompatible plant and has high rates of infertility; its genetic diversity only occurs via processes of mutation and natural selection. Since ginger is important as a condiment and as an herbal medicine, understanding its diversity to a greater degree can contribute both to its conservation and to its use in ... more

E.S. Cardoso; A.A.B. Rossi; E.C.M. Pedri; K.É.M. Zortéa; A.V. Tiago; V.D. Rocha; A.S. Rodrigues; U.A. Oliveira