Research Article

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06/29/2002
Apomixis; Apospory; Heterosis; Interspecific hybridization; Triploid

Apomixis means seed formation without fertilization. In cassava (Manihot esculenta) it is an alternative to reproduction by cuttings, which normally transmits pathogens and leads to an accumulation of viral and bacterial diseases. Apomixis also assures preservation of heterosis and avoids genetic segregation. It occurs in wild relatives of cassava and has been transferred ... more

N.M.A. Nassar
12/11/2007
Amino acids; Apomixis; Drought; Protein

Wild cassava relatives are perennials and vary in growth pattern from nearly acaulescent subshrubs to small trees. They have been used as a source of useful characters such as high protein content, apomixis, resistance to mealybug and mosaic disease, and tolerance to drought. Indigenous clones are a potential source of β-carotene and lycopene. Apomixis genes have been transferred to the crop ... more

N.M.A. Nassar
08/11/2006
Apomixis; Interspecific crosses; Polyploidy

A new species was synthesized artificially by chromosome doubling in a hybrid. The ensuing polyploid type exhibits an apomictic nature and maintains its morphological characteristics in the progeny. It showed a frequency of multiembryonic sacs of 29% in the ovules examined, whereas sacs were absent in the diploid type.

*In honor of deceased Professor Vieira who dedicated a part ... more

N.M.A. Nassar
11/29/2005
Apomixis; Molecular marker; Satellite technique

Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using five ... more

N.M.A. Nassar; R.G. Collevatti
11/03/2009
Aneuploidy; Apomixis; interspecific hybrid; Wild species

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with ... more

N.M.A. Nassar; P.T.C. Gomes; A.M. Chaib; N.N. Bomfim; R.C.D. Batista; R.G. Collevatti
10/05/2011
Apomixis; Callose; Embryology; Flow cytometry screen seed; Sexual reproduction

Apomixis is a particular mode of reproduction that allows progeny formation without meiosis and fertilization. Eulaliopsis binata, a tetraploid apomictic species, is widely used for making paper, rope and mats. There is great potential for fixation of heterosis in E. binata due to autonomous endosperm formation in this species. Although most of its embryo sac originates ... more

L. Liu; J.J. Li; Y.D. Ouyang; J.L. Yao
10/22/2013
Apical meristems; Apomixis; Epigenetic; Pollen viability; Root productivity

Cassava periclinal cytochimeras, cultivars, and interspecific hybrid and polyploid types were studied in relation to embryonic, cytogenetic, and anatomical behavior. Their apical shoots, pollen grains, male and female buds, roots, stomata, and flowering period were analyzed. Chimeras exhibited increased size of L1 and L2 cells. Polyploidy led to enlargement of stomata in chimeras whereas ... more

D.Y. Hashimoto-Freitas; N.M.A. Nassar
12/11/2007
Bovidae; Cervidae; Cross-species amplification; Microsatellite; Pampas deer

In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, ... more

J.E. Maldonado; M. Cosse; S. González
06/20/2007
Heterozygosity; Leopardus pardalis; Leopardus tigrinus; Leopardus wiedii; Microsatellite

The microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive Leopardus sp. The PCR-amplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and ... more

A.B. Grisolia; V.R. Moreno; F. Campagnari; M.P. Milazzotto; J.F. Garcia; C.H. Adania; E.B. Souza
06/20/2007
Canidae; Chrysocyon brachyurus; Cross-amplification; Domestic dog; Microsatellite; Population genetics

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have ... more

D.C. Salim; A.A. Akimoto; C.B. Carvalho; S.F. Oliveira; C.K. Grisolia; J.R. Moreira; M.N. Klautau-Guimarães

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