Research Article

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Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase gene, was cloned from ... more

X.G. Li; J. Wang; Z.Y. Yu
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Erythropoietin; Gene expression; Hypoxic adaptation; Tibetan pig

This study aimed to observe the expression characteristics of the erythropoietin (EPO) gene in different tissues of Tibetan pigs and to explore the adaptation to hypoxic environments. The cDNA in heart, liver, lung, kidney, muscle, brain, and fat of Tibetan pigs was used as the template. Through the number of cycles of the polymerase chain reaction (PCR), annealing temperature, ... more

B.Z. Deji; P. Shang; W.J. Danzeng; H. Zhang; Y.Z. Qiangba
02/06/2015
Chicken; Fat-related traits; Gene expression; Housing system

Free-range production system is increasingly being used in poultry breeding and feed production in many countries. The objective of the current experiment was to evaluate the effects of different raising systems on fat-related traits and mRNA levels of liver lipogenesis genes in Erlang Mountainous chicken. Each of 10 birds (91 day old) from caged, indoor-floor housed, and free-range ... more

Q. Li; X.L. Zhao; E.R. Gilbert; Y.P. Liu; Y. Wang; M.H. Qiu; Q. Zhu
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Gene expression; Sheep; Skeletal muscle; Transcriptome

The objectives of the present study were to identify additional genes that may play important roles in the regulation of skeletal muscle growth and development, and to provide fundamental information for understanding the underlying molecular mechanisms. Eighteen cDNA libraries were constructed from the longissimus muscle of Polled Dorset (PD) and Small-tailed Han (SH) fetuses. To reveal ... more

N. Liu; J.N. He; W.M. Yu; K.D. Liu; M. Cheng; J.F. Liu; Y.H. He; J.S. Zhao; X.X. Qu
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The cucumber (Cucumis sativus L.) is an important crop grown worldwide. In this study, the genetic diversity of 42 cucumber cultivars in China was analyzed using 51 pairs of simple sequence repeat (SSR) primers. These primers identified 129 polymorphic loci, 95.6% of which were polymorphic. The mean effective number of alleles, mean Nei’s gene diversity, and mean Shannon’s ... more

Y.T. Yang; Y. Liu; F. Qi; L.L. Xu; X.Z. Li; L.J. Cong; X. Guo; S.X. Chen; Y.L. Fang
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Abiotic stress tolerance; Anti-oxidative enzymes; Cu/Zn-SOD; Jatropha curcas; NaCl stress

Superoxide dismutases (SODs) are involved in protecting plants against diverse biotic and abiotic stresses. In the present study, a novel Cu/Zn-SOD gene (JcCu/Zn-SOD) was cloned from Jatropha curcas L. Quantitative reverse transcription-polymerase chain reaction analysis revealed that JcCu/Zn-SOD is constitutively expressed in different tissues of J ... more

Z.B. Liu; W.J. Zhang; X.D. Gong; Q. Zhang; L.R. Zhou
06/11/2015
5-aminolevulinic acid; Aquaporin; Salinity stress; Tomato

The effects of exogenous 5-aminolevulinic acid (ALA) on photosynthesis, plant growth, and the expression of two aquaporin genes in tomato seedlings under control and salinity conditions were investigated. Exogenous ALA application significantly improved net photosynthetic rate (Pn), total chlorophyll content, and plant biomass accumulation of tomato seedlings under ... more

Y.Y. Zhao; F. Yan; L.P. Hu; X.T. Zhou; Z.R. Zou; L.R. Cui
12/21/2015
Apoptosis; Aquaporin; Cell proliferation; Diabetes; Myocardial cell

The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric analysis of Annexin V-fluorescein ... more

P.F. Zhao; M.J. Sun

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