Research Article

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Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component comparisons of microsatellite allele sizes allowed the ... more

P. Ripamonte; M. Baccaglini; A.S.M. César; A.S.M. César; L.G.G. Figueiredo; J.C.C. Balieiro; A.R. Caetano; F.V. Meirelles
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Cattle; Haplotype; Polymorphism; Short tandem repeat; Y-chromosome

The aim of this study is to use Y-chromosome gene polymorphism method to investigate regional differences in genetic variation and population evolution history of the Chinese native cattle breeds. Six Y-chromosome short tandem repeat (Y-STR) loci (UMN0929, UMN0108, UMN0920, INRA124, UMN2404, and UMN0103) were analyzed using 1016 healthy ... more

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Bos taurus indicus; Cattle; InDel; Polymorphism; Traceback

Brazil is a major producer and exporter of beef, with a herd of approximately 210 million animals. For the meat industry, a reliable animal traceback from its origin to the consumer market is paramount. Of all available identification systems, DNA is the only one that survives the slaughterhouse and reaches the dish of the consumer. DNA polymorphisms are already used for cattle traceback ... more

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Carcass composition trait; Cattle; Growth trait; Meat quality trait; Polymorphism; Thyroglobulin gene

Thyroid hormones play an important role in regulating metabolism and can affect metabolism-related traits such as fat deposition. The thyroglobulin (TG) gene produces the precursor of thyroid hormones and has been proposed as a candidate gene for a quantitative trait locus with an effect on fat deposition. In this study, we identified 4 novel single nucleotide polymorphisms (SNPs ... more

L.P. Zhang; Q.F. Gan; G.Y. Hou; H.J. Gao; J.Y. Li; S.Z. Xu
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Cattle; Cross-reactivity; Cytokines; Goat; Human; Sheep

Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated ... more

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AMPD1 gene; Cattle; Deletion mutation; Production traits

The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. The five 19-bp polymerase chain reaction products of the AMPD1 gene exhibited 3 genotypes and 2 alleles: WW: homozygote ... more

C.B. Wei; J.Q. Wang; F.Y. Chen; H. Niu; K. Li
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Adipose tissue; Buffalo; Cattle; Cell therapy; Mesenchymal stem cells

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic ... more

R.V. Sampaio; M.R. Chiaratti; D.C.N. Santos; F.F. Bressan; J.R. Sangalli; A.L.A. Sá; T.V.G. Silva; N.N. Costa; M.S. Cordeiro; S.S.D. Santos; C.E. Ambrósio; P.R. Adona; F.V. Meirelles; M.S. Miranda; O.M. Ohashi
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Frequencies of κ-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Gyr cows, Guzerat sires, Guzerat cows, Nellore sires, and Gyr x Holstein crossbreds) by means of polymerase chain reaction-restriction fragment length polymorphism analysis using two independent restriction nucleases (Hinf I and HaeIII). ... more

A.L.S. Azevedo; C.S. Nascimento; R.S. Steinberg; M.R.S. Carvalho; M.G.C.D. Peixoto; R.L. Teodoro; R.S. Verneque; S.E.F. Guimarães; M.A. Machado
10/13/2010
Bovine; Candidate genes; Fat deposition; SNP identification

Canchim is a composite cattle breed developed in Brazil for beef production. One of the breeding objectives is to increase fat deposition. QTLs for fat thickness and/or marbling have been reported on BTA4 and BTA14. The IGFBP3 and DDEF1 genes, mapped to BTA4 and BTA14, respectively, affect adipogenesis. We looked for SNPs in the IGFBP3 and DDEF1 genes that could be associated with backfat ... more

G.B. Veneroni; S.L. Meirelles; D.A. Grossi; G. Gasparin; A.M.G. Ibelli; P.C. Tizioto; H.N. Oliveira; M.M. Alencar; L.C.A. Regitano

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