Research Article

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11/08/2011
DNA extraction; Medicinal plant; Molecular study; TriOmicTM

DNA extraction was carried out on 32 medicinal plant samples available in Malaysia using the TriOmicTM extraction kit. Amounts of 0.1 g flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1plus buffer and followed by RNAse treatment. Then, RY2 buffer was added to the samples and mixed completely by vortexing before removal of cell debris by ... more

A.R. Mohd-Hairul; A.B. Sade; B.C. Yiap; A.R. Raha
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Cereal seeds; DNA extraction; PCR

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves ... more

Z.H. Pervaiz; N.A. Turi; I. Khaliq; M.A. Rabbani; S.A. Malik
03/29/2011
DNA extraction; PCR; Protocol

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single ... more

T.Y. Wang; L. Wang; J.H. Zhang; W.H. Dong
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Cladosporium spp; DNA extraction; PCR; rDNA-ITS1

We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted DNA from mycelium mats after manual or electric homogenization. ... more

M.A. Moslem; A.H. Bahkali; K.A. Abd-Elsalam; P.J.G.M. Wit
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Buccal swab brushes; DNA extraction; Elderly; Epidemiologic studies

We evaluated the cost-effectiveness of using buccal swab brushes in comparison with blood samples for obtaining DNA for large epidemiological studies of the elderly population. The data reported here are from the third phase of the Integral Study of Depression among the Elderly in Mexico City’s Mexican Institute of Social Security, conducted in 2007. The total cost of the two procedures ... more

T. Juárez-Cedillo; S. Sánchez-García; J.F. Mould-Quevedo; C. García-Peña; J.J. Gallo; F.A. Wagner; G. Vargas-Alarcón
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CTAB; DNA extraction; Herbarium specimen; Papilionoideae; PCR amplification

Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with cetyl trimethylammonium bromide (CTAB); a third protocol used a Nucleospin Plant kit. DNA obtained from all three procedures was ... more

M. Riahi; S. Zarre; A.A. Maassoumi; F. Attar; K. Osaloo
10/22/2013
Apis mellifera; DNA extraction; Lysis buffer; Polyacrylamide gel; Proteinase K; Varroa destructor

We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, thoracic muscle ... more

M.R.C. Issa; V.L.C. Figueiredo; D. De Jong; C.H. Sakamoto; Z.L.P. Simões
08/21/2013
Castor bean; DNA extraction; Endophytic microbiota; Metagenomic; Selective PCR

Ricinus communis (castor bean) seeds are used to produce an alcohol-soluble oil that is used in more than 400 industrial processes. Despite its economic importance, there has been little research on the endophytic microbiota of castor bean seeds. This microbiota is important for plant metabolic processes and may have considerable biotechnological potential, such as production of ... more

C.D. Santos; A.C.C. Dias; I.M.R. Amaral; A.M. Bonetti; T.A. Campos
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Clotted blood; DNA extraction; RNA extraction; Sonication

We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were adequate for gene expression ... more

Z. Zakaria; S.H. Umi; S.S. Mokhtar; U. Mokhtar; M.Z. Zaiharina; A.T.A. Aziz; B.P. Hoh
12/19/2012
DNA extraction; Leguminosae; RAPD; SRAP; SSR

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and ... more

I.O. Agbagwa; S. Datta; P.G. Patil; P. Singh; N. Nadarajan

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