Research Article

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12/04/2014
Cloning; Date palm; Maturase K (matK); Ribulose bisphosphate carboxylase (rbcL); Sequencing; Taxonomy

DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, ... more

A. Naeem; A.A. Khan; H.M.N. Cheema; I.A. Khan; A. Buerkert
06/01/2015
Bioinformatic analys; Cloning

Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. To study the structure and function of the S. agalactiae neuA gene, we isolated it from diseased tilapia, amplified it ... more

E.L. Wang; K.Y. Wang; D.F. Chen; Y. Geng; L.Y. Huang; J. Wang; Y. He
04/10/2015
Chicken; Cloning; R-spondin1; Tissue distribution

Rspo1 belongs to the Rspo family, which is composed of 4 members (Rspo1-4) that share 40 to 60% sequence homology and similar domain organizations, and regulate the WNT signaling pathway via a common mechanism. Rspo1 plays a key role in vertebrate development and is an effective mitogenic factor of gastrointestinal epithelial cells. We report the cloning ... more

Y.Q. Han; J. Geng; H.T. Shi; X.M. Zhang; L.L. Du; F.T. Liu; M.M. Li; X.T. Wang; Y.Y. Wang; G.Y. Yang
09/22/2011
Aleyrodidae; Genetic diversity; Insecta; Molecular characterization; Whitefly

Bemisia tabaci (Hemiptera: Aleyrodidae) consists of a complex of morphologically indistinct biotypes that vary mainly in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The adaptability of B. tabaci to many regions of the world has fostered the appearance of various biotypes and has resulted in a broad spectrum of ... more

G.E. Valle; A.L. Lourenção; M.I. Zucchi; J.B. Pinheiro; A.G. Abreu
10/05/2010
Dendogram; Molecular characterization; RAPD; Similarity index; Vitis vinifera L.

Turkey is very rich in local grape varieties. The solution to the problem of identifying local cultivars, which is considered an important deficiency for the region, will only be possible when they can be defined with molecular markers. Forty-nine local grapevine cultivars from Şanlıurfa (Turkey) were characterized with RAPD markers. Twenty-five decamer primers selected from 60 primers ... more

H. Karataş; Y.S. Ağaoğlu
04/20/2010
Exclusion probability; Fingerprinting; Genotyping method; Glycine max; Molecular characterization; Random identity probability

The narrow genetic base of soybean makes cultivar characterization based on morphological descriptors difficult; this characterization is mainly done for registration and protection. Correct characterization of cultivars could be achieved through molecular markers, since the frequencies of each allele in the population are known. Consequently, we developed a molecular characterization ... more

M.B. Oliveira; E.S.N. Vieira; I. Schuster
08/16/2012
Barcode; Cytochrome oxidase I; Didelphis; Molecular characterization; Sympatry

We made a molecular study of 40 opossums, Didelphis albiventris, from an urban fragment of the Atlantic Rainforest in southeastern Brazil, analyzing a 653-bp sequence of cytochrome c oxidase, subunit I. We found three close connected haplotypes, with low nucleotide diversity and a haplotype diversity of 59.1% and confirmed sympatry between D. albiventris and D. ... more

L.C.C. Sousa; C.M.F. Gontijo; G.A. Lacorte; S.N. Meireles; A.P. Silva; C.G. Fonseca
02/10/2012
Colombia; Microsatellites; Molecular characterization; Rubus glaucus; SSRs; Thornless blackberry

The species Rubus glaucus, also known as the Andean or “Castilla” blackberry, is one of nine edible species of this genus that grow naturally in Central and South America. In Colombia, this species is the most important of all Rubus species for agricultural and commercial purposes. We used 20 SSRs developed for other Rubus species to characterize 44 Colombian ... more

M. Marulanda; A.M. López; M. Uribe
09/12/2014
Diversity; Domestication; Germplasm bank; ISSR; Molecular characterization; Morphological characterization

Capsicum chinense and C. frutescens peppers are part of the Brazilian biodiversity, and the Amazon basin is the area of greatest diversity for them, especially for that former species. Nevertheless, little is known about their evolutionary history. Aiming to identify genotypes with wild and domesticated characteristics, 30 accessions of the germplasm bank of Embrapa ... more

S.I.C. Carvalho; C.F. Ragassi; L.B. Bianchetti; F.J.B. Reifschneider; G.S.C. Buso; F.G. Faleiro
01/14/2014
Molecular characterization; Pomegranate; Punica granatum; RAPD

The pomegranate is one of the oldest fruits that are traditionally consumed by the local inhabitants of the Coruh Valley, Turkey. In this study, the molecular and morphological characteristics of 19 promising pomegranate genotypes selected from the Coruh Valley were evaluated. For the morphological evaluation, 22 quantitative fruit characteristics were used. For the molecular evaluation, ... more

E. Orhan; S. Ercisli; A. Esitken; M. Sengul

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