Research Article

Related GMR Articles

07/24/2013
cDNA-AFLP; Floral bud abortion; Gene expression; Radish; Real-time PCR

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain ... more

J. Zhang; X.L. Sun; L.G. Zhang; M.X. Hui; M.K. Zhang
04/10/2013
Colorectal cancer; DNA hypomethylation; Epigenetics; Gene expression; Metastasis

DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloproteinase-2 (TIMP-2) is known to ... more

Z. Hussain; M.I. Khan; M. Shahid; F.N. Almajhdi
04/02/2013
Apoptosis; Bladder cancer; Gene expression; RT-PCR

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder ... more

D.F. Barione; F.S.N. Lizarte; P.C. Novais; C.A.M. de Carvalho; F.C.B. Valeri; F.M. Peria; H.F. de Oliveira; D.L. Zanette; W.A. Silva; A.J. Cologna; R.B. Reis; S. Tucci; A.C.P. Martins; D.P.C. Tirapelli; L.F. Tirapelli
01/24/2013
CTAB-LiCl; Gene expression; Nelumbo nucifera; RNA isolation; RT-PCR

Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-quality RNA ... more

Y.J. Zhang; X.Y. Hao; Z.S. Liang; W.D. Ke; H.B. Guo
09/17/2012
Conjugated linoleic acid; Dairy cows; Gene expression; Milk fat synthesis; Sterol response element binding protein 1

The efficacy of conjugated linoleic acid (CLA) in diet supplements for milk fat reduction is well documented in several species. However, the mechanisms by which fatty acids regulate mammary lipogenesis remain largely unknown, especially with regard to gene expression of enzyme and regulators. In this study, 8 Holstein dairy cows in their mid-lactation period were randomly divided into 2 ... more

L.Q. Han; K. Pang; H.J. Li; S.B. Zhu; L.F. Wang; Y.B. Wang; G.Q. Yang; G.Y. Yang
11/12/2012
Gene expression; globin genes; Human erythropoiesis; Phosphatidylinositol phosphate kinases

Phosphatidylinositol phosphate kinases (PIPKs) are enzymes that participate in diverse intracellular signaling pathways. They are classified into 3 functionally distinct subfamilies - PIPKI (a, b, g), PIPKII (a, b, g), and PIPKIII - located in various subcellular compartments. Recently, the PIPKIIa and b-globin genes were found to be overexpressed in reticulocytes from 2 siblings with ... more

T.R. Zaccariotto; C. Lanaro; D.M. Albuquerque; M.N.N. Santos; M.A.C. Bezerra; F.G.P. Cunha; I. Lorand-Metze; A.S. Araujo; F.F. Costa; M.F. Sonati
09/13/2012
Dithranol; Gene expression; HaCaT; Id1; Psoriasis

The precise causes of psoriasis, a chronic skin disorder characterized by hyperproliferation of keratinocytes and incomplete keratinization, are unclear. It is known that expression of helix-loop-helix transcription factor Id1, which functions as an inhibitor of differentiation, is upregulated in psoriatic skin. We investigated the effect of the antipsoriatic drug dithranol on mRNA and ... more

C. Ronpirin; T. Tencomnao
09/03/2012
Coffee; Gene expression; Maturation; PME; Quality

Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring ... more

S.M.B. Cação; T.F. Leite; I.G.F. Budzinski; T.Bdos Santos; M.B.S. Scholz; V. Carpentieri-Pipolo; D.S. Domingues; L.G.E. Vieira; L.F.P. Pereira
08/16/2012
Gene expression; Heat shock protein 90; heavy metal; Phascolosoma esculenta; Western blot

The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the characteristic motifs of ... more

C.H. Li; X.R. Su; M.Q. Wang; Y.Y. Li; Y. Li; J. Zhou; T.W. Li
08/06/2012
Gene expression; HSP104; Protein structure; Trypanosoma cruzi

Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a single-copy ... more

R.A. Campos; M.L. da Silva; G.V. da Costa; P.M. Bisch; J.M. Peralta; R. Silva; E. Rondinelli; T.P. Ürményi

Pages