Research Article

Related GMR Articles

05/15/2002
Apis mellifera; Honey bee; Intranuclear acid phosphatase activity; Post-embryonic development

We report nuclear acid phosphatase activity in the somatic (intra-ovariolar and stromatic) and germ cells of differentiating honey bee worker ovaries, as well as in the midgut cells of metamorphosing bees. There was heterogeneity in the intensity and distribution of electron dense deposits of lead phosphate, indicative of acid phosphatase activity in the nuclei of these tissues, during ... more

Cda Cruz Landim; R.Daniele Reginato; R.Lucia More de Moraes; V.Melo Cavalcante
01/25/2003
Africanized; Apis mellifera; Effective reproduction; Fertility; Reproduction; Varroa

Varroa destructor reproductive success is considered an important character for determining the resistance of honey bees to this mite parasite. However, most of the published data are not comparable due to the different methods of ascertaining and reporting reproduction. A recently published technique that involves reconstructing mite families in older worker brood gives ... more

M.H. Corrêa-Marques; L.Medina Medina; S.J. Martin; D. De Jong
03/31/2005
Apis mellifera; Honey bees; Hygienic behavior; Removing; Uncapping

Most research on hygienic behavior has recorded the time taken by the colony to remove an experimental amount of dead brood, usually after one or two days. We evaluated the time that hygienic (H) and non-hygienic (NH) honey bees take to uncap and remove dead brood in observation hives after the brood was killed using the pin-killing assay. Four experimental colonies were selected as ... more

M.Alejandra Palacio; J.Manuel Flores; E. Figini; S. Ruffinengo; A. Escande; E. Bedascarrasbure; E. Rodriguez; L.Segui Gonçalves
10/13/2009
Africanized; Apis mellifera; Bee nutrition; Bradford; Protein diets

The superiority of Africanized over European honey bees in tropical and subtropical regions of the New World is both well documented and poorly understood. As part of an effort to try to understand the process by which the displacement of European bees occurred, we examined the ability of these two types of bees and of hybrids between the two to convert natural and artificial diets into ... more

F.A. Cappelari; A.P. Turcatto; M.M. Morais; D. De Jong
06/30/2009
Africanized honey bees; Apis mellifera; body fluid; Carniolan honey bees; Hygienic behavior; pin-killing method

In Apis mellifera, hygienic behavior involves recognition and removal of sick, damaged or dead brood from capped cells. We investigated whether bees react in the same way to grouped versus isolated damaged capped brood cells. Three colonies of wild-type Africanized honey bees and three colonies of Carniolan honey bees were used for this investigation. Capped worker brood cells aged 12 ... more

K.P. Gramacho; L.S. Gonçalves
06/23/2009
Africanized honey bee; Apis mellifera; Hygienic behavior; Observation hives

The hygienic behavior of honey bees is based on a two-step process, including uncapping and removing diseased, dead, damaged, or parasitized brood inside the cell. We evaluated during periods of 1 h the time that hygienic and non-hygienic colonies of Africanized honey bees spend to detect, uncap and remove pin-killed brood using comb inserts with transparent walls placed in observation hives. ... more

M.M. Morais; T.M. Francoy; R.A. Pereira; D. De Jong; L.S. Gonçalves
06/16/2009
Apis mellifera; Biodiversity; Geometric morphometrics; mtDNA; species identification; Wing morphometrics

Though the replacement of European bees by Africanized honey bees in tropical America has attracted considerable attention, little is known about the temporal changes in morphological and genetic characteristics in these bee populations. We examined the changes in the morphometric and genetic profiles of an Africanized honey bee population collected near where the original African swarms ... more

T.M. Francoy; D. Wittmann; V. Steinhage; M. Drauschke; S. Müller; D.R. Cunha; A.M. Nascimento; V.L.C. Figueiredo; Z.L.P. Simões; D. De Jong; M.C. Arias; L.S. Gonçalves
12/19/2013
Apis mellifera; Hemolymph; Nutrition; Pollen substitute; Protein diets

Pollen substitute diets are a valuable resource for maintaining strong and health honey bee colonies. Specific diets may be useful in one region or country and inadequate or economically unviable in others. We compared two artificial protein diets that had been formulated from locally-available ingredients in Brazil with bee bread and a non-protein sucrose diet. Groups of 100 newly- ... more

M.M. Morais; A.P. Turcatto; R.A. Pereira; T.M. Francoy; K.R. Guidugli-Lazzarini; L.S. Gonçalves; J.M.V. de Almeida; J.D. Ellis; D. De Jong
11/07/2013
Apis mellifera; Body weight; Longevity; Queen; Reproduction

There has been much speculation about which phenotypic traits serve as reliable indicators of productivity in queen honeybees (Apis mellifera). To investigate the predictive value of queen body weight on colony development and quality, we compared colonies in which queens weighed less than 180 mg to those in which queens weighed more than 200 mg. Both groups contained naturally ... more

D.A. De Souza; M.A.F. Bezzera-Laure; T.M. Francoy; L.S. Gonçalves
10/22/2013
Apis mellifera; DNA extraction; Lysis buffer; Polyacrylamide gel; Proteinase K; Varroa destructor

We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, thoracic muscle ... more

M.R.C. Issa; V.L.C. Figueiredo; D. De Jong; C.H. Sakamoto; Z.L.P. Simões

Pages