Research Article

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06/16/2014
DNA isolation; Polymerase chain reaction; Protocol

Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or ... more

C.C. Sousa; S.O. Gomes; A.C.A. Lopes; R.L.F. Gomes; F.B. Britto; P.S.C. Lima; S.E.S. Valente
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Cut-and-paste-based cloning strategy; p150Glued; Polymerase chain reaction; Site-directed mutagenesis

Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be performed with ease. However, site-directed ... more

C. Wang; T.Y. Wang; L.Y. Zhang; X.J. Gao; X.W. Wang; C.J. Jin
01/30/2015
Extraction efficiency; Freshwater; Microbial diversity; Polymerase chain reaction; Total DNA

The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane ... more

P. Li; S.F. Yang; B.B. Lv; K. Zhao; M.F. Lin; S. Zhou; X. Song; X.M. Tang
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25S rDNA-PCR; Candida albicans; Polymerase chain reaction; Random amplified polymorphic DNA

We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this ... more

N. Wu; J. Lin; L. Wu; J. Zhao
01/30/2015
Arachis hypogaea; Black aspergilla; Loop-mediated isothermal amplification; Ochratoxins; Polymerase chain reaction

Over the last decade, ochratoxin A (OTA) has been widely described and is ubiquitous in several agricultural products. Ochratoxins represent the second-most important mycotoxin group after aflatoxins. A total of 34 samples were surveyed from 3 locations, including Mecca, Madina, and Riyadh, Saudi Arabia, during 2012. Fungal contamination frequency was determined for surface-sterilized ... more

H.M. Al-Sheikh
08/27/2007
Cell suspension; Cytogenetic; DNA extraction; DNAzol

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, ... more

M.R. Amorim; F.R. Vargas; J.C.Llerena Junior; M.S. Pombo-de-Oliveira
03/29/2007
Dalbergia species; DNA extraction; Herbarium specimens; PCR amplification

Five published DNA extraction protocols were compared for their ability to produce good quality DNA from fresh and herbarium leaves of several species of the genus Dalbergia. The leaves of these species contain high amounts of secondary metabolites, which make it difficult to perform a clean DNA extraction and thereby interfering with subsequent PCR amplification. The protocol ... more

R.A. Ribeiro; M.B. Lovato
03/01/2011
Cerrado; Dimorphandra mollis; DNA extraction; Metabolic compounds; “Fava d’anta”

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during ... more

P.A. Moreira; D.A. Oliveira
02/04/2013
16S rRNA; Bacterial consortium; DNA extraction; Electrical energy; Inexpensive DNA protocol; Microbial fuel cell

Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs. ... more

B. Canto-Canché; M. Tzec-Simá; J.I. Vázquez-Loría; H. Espadas-Álvarez; B.H. Chí-Manzanero; R. Rojas-Herrera; R. Valdez-Ojeda; L. Alzate-Gaviria
08/13/2012
DNA extraction; DNA isolation kits; Grapevine

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols and three commercial DNA ... more

M. Akkurt

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