Research Article

Related GMR Articles

Endothelial cells; Interleukin-6; Tumor necrosis factor alpha; Vascular smooth muscle cell

The aim of this study was to investigate the influence of activated endothelial cells on the proliferation and secretion of vas­cular smooth muscle cells (VSMCs). Cultured lung microvascular endothelial cells were treated with or without tumor necrosis factor alpha (TNF-α; 10 ng/mL) for 6 h, and the supernatant was collected and filtered. The supernatant with TNF-α was called fluid A, and ... more

H.P. Tang; L.X. Sun; W. Han
Cyclophilin-D protein; Endothelial cells; Oxidative stress

We examined the influence of cyclophilin-D (CypD) protein expression level on endothelial cell oxidative damage resistance. A model of CypD protein expression or high expression in endothelial cells was established through gene silencing or cloning. The comparable groups were normal endothelial cells cultured in phosphate-buffered solution in liquid handling cells containing 500 mM H ... more

J.Z. Peng; L. Xue; J. Chen; B.S. Chen; Y.Q. Yang
Endothelial cells; High glucose; Inflammation; NADPH oxidase; NF-κB; Retinoid X receptor

An inflammatory response induced by high glucose is a cause of endothelial dysfunction in diabetes and is an important contributing link to atherosclerosis. Diabetes is an independent risk factor of atherosclerosis and activation of retinoid X receptor (RXR) has been shown to exert anti-atherogenic effects. In the present study, we examined the effects of the RXR ligands 9-cis- ... more

R.B. Ning; J. Zhu; D.J. Chai; C.S. Xu; H. Xie; X.Y. Lin; J.Z. Zeng; J.X. Lin
Adult; Aged; Cytokines; Endothelial cells; Female; Fibroblasts; Humans; Macrophages; male; Mast Cells; Middle Aged; Periapical Diseases; Periapical Granuloma; Periapical Tissue; Radicular Cyst; Stem Cell Factor

Stem cell factor (SCF), an important stem cell cytokine, has multiple functions. Fibroblasts (FBs), mature mast cells, endothelial cells (ECs), and eosinophil granulocytes can produce SCF in the inflammatory process. Therefore, we aimed to observe SCF expression in FBs, ECs, and macrophages (MPs) in periapical tissues in human chronic periapical disease and investigate the effects of cells ... more

S.Q. Shen; R. Wang; S.G. Huang
AQP-1; Barrier function; Hydrogen; Lung; Rat; Sepsis

This study aimed to explore the protective effect of hydrogen and to investigate the underlying mechanism of its preliminary effect on the alveolar epithelial barrier function in septic rats. Forty-five male Sprague-Dawley rats were divided randomly into three groups (N = 15): control [saline injection (intraperitoneal, ip), air drawing; SA], acute lung injury group [ ... more

L.D. Liu; X.Y. Wu; B.D. Tao; N. Wang; J. Zhang; L.D. Liu; X.Y. Wu; B.D. Tao; N. Wang; J. Zhang; L.D. Liu; X.Y. Wu; B.D. Tao; N. Wang; J. Zhang
Dairy cow; Lipid metabolism genes; Lipopolysaccharide; Primary hepatocyte; Toll-like receptor 4 signaling pathway

The influence of ruminal acidosis on ruminal microbiology and metabolite production has received considerable attention, but little is known regarding the systemic manifestations that arise from ruminal acidosis. Lipopolysaccharide (LPS) is released in the gastrointestinal tract upon ingestion of high-grain or high-fat diets, and it has been implicated in the etiology of multiple energy- ... more

Y.Y. Wang; H.P. Li; X.J. Wang; Q.D. Jiang; J. Geng; L.F. Wang; Y.J. Guo; W.F. Lu; H.S. Zhu; G.Y. Yang
Chicken; Host defense peptide; Lipopolysaccharide; Vitamin D3; β-defensin

The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At ... more

L. Lu; S.M. Li; L. Zhang; X.Q. Liu; D.Y. Li; X.L. Zhao; Y.P. Liu
Lipopolysaccharide; Low rumen pH; Proteomics

The aim of this study was to analyze the response of dry goat liver to sub-acute ruminal acidosis induced by a highly concentrated diet. Non-pregnant, non-lactating female Poll-goats (N = 12) were randomly assigned to either a high-concentrate (HG) or a low-concentrate (LG) diet. Low rumen pH was successfully induced with HG (more than 3 h with rumen pH S-transferase was downregulated in ... more

Z. Xie; X. Jiang; P. Ye; Y. Zhang; Y. Ni; S. Zhuang; X. Shen
Cytokines; GILZ; Glucocorticoid; Protein metabolism; Sepsis

Sepsis is a complex inflammatory response to infection, associating with dramatic metabolic disorders. Although the mechanisms of immune response during sepsis have been largely clarified, current studies rarely pay attention to the disordered protein metabolism in sepsis. In this study, L6 rat skeletal muscle cells treated with serum from septic rats were used as an in vitro ... more

J. Xiong; L. Xu; W.M. Qu; Z.L. Li; Z.H. Shang; Y.H. Li; S.H. Yang; Z.H. Yang
Differentially expressed genes; Functional enrichment analysis; Interaction network; Multiple trauma; Pathway analysis; Sepsis

The purpose of this study was to identify critical genes associated with septic multiple trauma by comparing peripheral whole blood samples from multiple trauma patients with and without sepsis. A microarray data set was downloaded from the Gene Expression Omnibus (GEO) database. This data set included 70 samples, 36 from multiple trauma patients with sepsis and 34 from multiple trauma ... more

S.C. Ji; Y.T. Pan; Q.Y. Lu; Z.Y. Sun; Y.Z. Liu