Research Article

Related GMR Articles

12/02/2013
Expression analysis; Hyriopsis cumingii; In situ hybridization; Laminin receptor precursor; Shell repair

Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability. This study focused on ... more

X.Z. Chang; J.L. Li; Z.Y. Bai; X.L. Li
11/18/2013
Carassius auratus var. Pingxiangnensis; Expression analysis; Immunofluorescence; Real-time PCR; ZP3

Carassius auratus var. Pingxiangnensis (designated CaP), distributed in the Pingxiang region of Jiangxi Province, China, is a natural, wild triploid crucian carp mutant that has two reproductive development modes: gynogenesis and bisexual reproduction. Little information is available about the expression pattern of the zona pellucida 3 (ZP3) gene during ovarian ... more

J.W. Shi; J.Q. Sheng; K. Peng; J.H. Wang; W.J. Yi; H.J. Wu; Q. Gu; Y.J. Hong
03/26/2014
CCoAOMT1 cloning; Chlorogenic acid synthetase; Expression analysis; Lonicera japonica Thunb.

Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein was detected by ... more

X.H. Jiang; C.W. She; Y.H. Zhu; X.M. Liu
06/18/2015
Expression analysis; Gene cloning; Nelumbo nucifera; NnAP1

The objective of this study was to clone the full-length cDNA of the APETALA1 (AP1) gene from lotus and analyze its sequence and expression pattern. The full-length cDNA sequence of the NnAP1 gene was amplified from the petals of Nelumbo nucifera ‘Hongxia’ using RT-PCR and rapid amplification of cDNA ends. Bioinformatic methods were used to analyze the ... more

D.Z. Kong; X.Y. Shen; B. Guo; J.X. Dong; Y.H. Li; Y.P. Liu
02/13/2015
CPP gene family; CXC domain; Expression analysis; Phylogenetic tree; Soybean

Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteine-rich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cell division in plants. In ... more

L. Zhang; H.K. Zhao; Y.M. Wang; C.P. Yuan; Y.Y. Zhang; H.Y. Li; X.F. Yan; Q.Y. Li; Y.S. Dong
01/23/2015
Capsicum annuum L.; CaRCCR gene; Cloning; Expression analysis; Stress response

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum ... more

H.J. Xiao; J.H. Jin; W.G. Chai; Z.H. Gong
04/04/2016
Checkpoint homolog 1; Cloning; Daphnia carinata; Expression; Whole-mount in situ hybridization

Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects ... more

L. Kong; H.X. Li; D.L. Wu; G.R. Xu; D.L. Wang; Y.L. Zhao; L. Kong; H.X. Li; D.L. Wu; G.R. Xu; D.L. Wang; Y.L. Zhao; L. Kong; H.X. Li; D.L. Wu; G.R. Xu; D.L. Wang; Y.L. Zhao