Research Article

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Caryocar brasiliense Camb. is a tree popularly known in Central Brazil as pequi. Its fruit contains carotenes, retinols, vitamin C, and polyphenols. These compounds possess antioxidant properties preventing excessive free radical formation and modulating the genotoxicity of physical and chemical agents in the body. However, at high concentrations these compounds can have ... more

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The search for new and effective antitumor agents with fewer cytotoxic side effects on normal tissue has increasingly become important. Lapachol, a natural organic compound isolated from the lapacho tree (Tabebuia avellandedae), is chemically identified as belonging to the naphthoquinone group and is known for its anti-inflammatory, analgesic and antibiotic properties, although ... more

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In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the ... more

Cda Silva Costa; F.Caixeta Albuquerque; R.Vieira Andrade; G.Camilo de Oliveira; M.Fernandes de Almeida; Mde Macedo Brigido; Mde Macedo Brigido; A.Queiróz Maranhão; A.Queiróz Maranhão
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CD4+ and CD25+ T cells; Colitis; Drug resistance; IL-8; Immune status; NOS activity

We examined the relationship among the multidrug resistance (MDR1) gene product P-glycoprotein (P-gp), ulcerative colitis, and immune status under ulcerative colitis. MDR1 P-gp expression and interleukin-8 levels in ulcerative colitis were determined using immunohistochemistry and a double-antibody sandwich avidin-biotin complex-enzyme-linked immunosorbent assay, respectively. ... more

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This study aimed to analyze the spectrum and drug resistance of bacteria isolated from burn patients to provide a reference for rational clinical use of antibiotics. Up to 1914 bacterial strain specimens isolated from burn patients admitted to hospital between 2001 and 2010 were subjected to resistance monitoring by using the K-B paper disk method. Retrospective analysis was performed on ... more

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Osteosarcoma is a primary malignant tumor in adolescents, associated with high mortality and morbidity. The high-dose methotrexate (MTX) chemotherapy used to treat this disease may induce primary or secondary drug resistance, resulting in a reduced effect of comprehensive treatment. In this study, the relationship between reduced folate carrier (RFC) gene expression and intracellular drug ... more

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Single nucleotide polymorphisms at codons 167, 198, and 200 in the β-tubulin isotype 1 gene have been associated with benz­imidazole resistance. Until now, the only mutation observed in Ancy­lostoma caninum was at codon 200 of this gene. However, the standard­ized methodologies used to detect mutations in this species are faulty. The objective of this study was to standardize a ... more

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Antigenotoxicity; Antioxidants; Doxorubicin; Drosophila melanogaster; SMART; β-carotene

β-carotene (BC), pro-vitamin A, is an efficient antioxidant, effective in the neutralization of oxygen reactive species, which cause serious damage to DNA. Various studies have been conducted on the effectiveness of BC for chemoprevention of cancer and heart disease. Doxorubicin is a chemotherapeutic agent used for cancer treatment that generates free radicals. We examined the effects of ... more

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Anthracycline-related cardiotoxicity; Anthracyclines; Cancer; Carbonyl reductase 1 (cbr1); Domestic dog (Canis lupus familiaris); Doxorubicin

The anticancer anthracyclines doxorubicin and daunorubicin are used to treat a variety of cancers in dogs. The therapeutic utility of anthracyclines is limited by cardiotoxicity in some cases. Synthesis of anthracycline alcohol metabolites by carbonyl reductase 1 (CBR1) is crucial for the pathogenesis of cardiotoxicity. We hypothesize that genetic polymorphisms in canine cbr1 ... more

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Cardiac myosin light-chain kinase; Doxorubicin; Myosin light-chain kinase; Na+-Ca2+ exchanger; Neuregulin

The aim of this study was to investigate the role of the rat neuregulin-1 (NRG-1) protein in reducing doxorubicin (DOX)-induced myocardial toxicity and its underlying mechanism. The prokaryotic expression of the NRG-1 protein and the CCK8-determined activity of rat primary myocardial cells were evaluated under different DOX concentrations. Myocardial cells were divided into three groups ... more

Y.Q. Liu; M. Yang; C.H. Duan; G.B. Su; J.H. Wang; Y.F. Liu; J. Zhang

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