Research Article

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Cloning; Litopenaeus vannamei; Ryanodine receptor; Single-nucleotide polymorphism

Ryanodine receptor/calcium release channel is a large protein that plays an essential role in muscle contraction; mutations in the ryanodine receptor gene affect sensitivity to stress. As a first step towards investigating the relationship between the ryanodine receptor and shrimp cramped muscle syndrome, we cloned, partially sequenced, and examined single-nucleotide polymorphisms (SNPs) ... more

X.H. Chen; D.G. Zeng; N. Ma
10/13/2010
IHHNV; Litopenaeus vannamei; Molecular diagnosis; RT-PCR; Shrimp aquaculture; Shrimp virus

The Pacific whiteleg shrimp Litopenaeus vannamei (Penaeidae) is one of the most important cultivated species in world aquaculture. In Brazil, the northeastern states are home to the main shrimp producers. As shrimp aquaculture has expanded and intensified, diseases have progressively become one of the most serious threats to this industry. Infectious hypodermal and hematopoietic ... more

M.A. Teixeira; J.E.F. Cruz; P.R.N. Vieira; I.R.C. Branco; F.H.F. Costa; G. Rádis-Baptista
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Asterias rubens; Gene expression; High-throughput sequencing; Real-time qPCR; Reference gene; UBA52

The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas. This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression at the transcriptional level is estimated by real- ... more

A.F. Sadritdinova; A.A. Dmitriev; A.V. Snezhkina; M.S. Belenikin; G.S. Krasnov; O.G. Manylov; A.A. Kudryavtsev; N.V. Melnikova; A.S. Speranskaya; M.V. Darii; V.A. Lakunina; L.A. Uroshlev; A.O. Smurov; O.A. Stepanov; A.V. Kudryavtseva
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Abiotic stress; Aluminum; Gene expression; MicroRNAs

MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe damage to plants. Although physiological aspects of aluminum ... more

J.C. Lima; R.A. Arenhart; M. Margis-Pinheiro; R. Margis
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Conservation; MicroRNAs; Target genes; Wheat

MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the plant kingdom, using the miRBase database. Some conserved miRNA ... more

Z.J. Yin; F.F. Shen
09/19/2013
EST analysis; MicroRNAs; Targets; Triticum aestivum L.

MicroRNAs (miRNAs) are a newly discovered class of noncoding small RNAs that regulate gene expression by directing target mRNA cleavage or translational inhibition. A large number of miRNAs have been identified in plants. Increasing evidence has shown that miRNAs play multiple roles in plant biological processes. So far, identification of miRNAs has been limited to a few model plant ... more

J. Han; M.L. Kong; H. Xie; Q.P. Sun; Z.J. Nan; Q.Z. Zhang; J.B. Pan
12/19/2012
Ab initio methods; MicroRNAs; Next-generation sequencing; Pseudo hairpins; Secondary structure

MicroRNAs (miRNAs) are short, non-coding RNA molecules that play an important role in the world of genes, especially in regulating the gene expression of target messenger RNAs through cleavage or translational repression of messenger RNA. Ab initio methods have become popular in computational miRNA detection. Most software tools are designed to distinguish miRNA precursors from ... more

L.L. Hu; Y. Huang; Q.C. Wang; Q. Zou; Y. Jiang
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Differential expression; Gastric cancer; MicroRNAs; Network analysis

To identify potential targets for the early treatment and prevention of gastric cancer, microRNA (miRNA) expression profiles of precancerous lesions of gastric cancer were investigated. The miRNA microarray dataset GSE24839 was downloaded from Gene Expression Omnibus (GEO) and included 10 Helicobacter pylori-related gastritis samples and 10 gastric intestinal metaplasia samples. ... more

X.W. Wang; Y. Wu; D. Wang; Z.F. Qin
10/20/2014
Computational prediction; Glycine max; MicroRNAs; Nodulation; Target genes

MicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process. We utilized a ... more

G.A. Barros-Carvalho; A.R. Paschoal; F.C. Marcelino-Guimarães; M. Hungria
01/21/2014
Computational identification; MicroRNAs; Phaseolus vulgaris; Target genes

MicroRNAs (miRNAs) are a class of non-coding small RNAs that negatively regulate gene expression at the post-transcriptional level. Although thousands of miRNAs have been identified in plants, limited information is available about miRNAs in Phaseolus vulgaris, despite it being an important food legume worldwide. The high conservation of plant miRNAs enables the identification of ... more

J. Han; H. Xie; M.L. Kong; Q.P. Sun; R.Z. Li; J.B. Pan

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