Research Article

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alpha-major regulatory element; Gene expression; globin genes; Hemoglobin; HS-40; thalassemia

Hemoglobin and globin genes are important models for studying protein and gene structure, function and regulation. We reviewed the main aspects of regulation of human α-globin synthesis, encoded by two adjacent genes (α2 and α1) clustered on chromosome 16. Their expression is controlled mainly by a regulatory element located 40 kb upstream on the same chromosome, the α- ... more

D.M. Ribeiro; M.F. Sonati
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Anatomy; DREB1A; Gene expression; Glycine max; Physiology; Water deficit

We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each ... more

A.M. Polizel; M.E. Medri; K. Nakashima; N. Yamanaka; J.R.B. Farias; M.C.N. de Oliveira; S.R.R. Marin; R.V. Abdelnoor; F.C. Marcelino-Guimarães; R. Fuganti; F.A. Rodrigues; R. Stolf-Moreira; M.A. Beneventi; A.A.P. Rolla; N. Neumaier; K. Yamaguchi-Shinozaki; J.F.C. Carvalho; A.L. Nepomuceno
11/22/2011
b-actin; DEP chip; Electrochemical biosensor; Gene expression; Hoechst 33258; RAGE

An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of β-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak current, and the results were compared with conventional agarose ... more

P. Kittimongkolsuk; T. Tencomnao; R. Santiyanont
12/08/2011
Drought stress tolerance; Gene expression; Real-time PCR; Soybean; Transcription factor

To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed ... more

S.S. Pereira; F.C.M. Guimarães; J.F.C. Carvalho; R. Stolf-Moreira; M.C.N. Oliveira; A.A.P. Rolla; J.R.B. Farias; N. Neumaier; A.L. Nepomuceno
11/10/2011
Abiotic stress; Aluminum; Gene expression; MicroRNAs

MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe damage to plants. Although physiological aspects of aluminum ... more

J.C. Lima; R.A. Arenhart; M. Margis-Pinheiro; R. Margis
10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
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cDNA array; Demineralized bone matrix; Gene expression; Human periosteal cells; Osteogenesis

Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA array technology. Osteogenic differentiation of HPO cells was ... more

S. Honsawek; P. Bumrungpanichthaworn; T. Thitiset; L. Wolfinbarger
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Adipocyte fatty acid-binding protein; Gene expression; Longissimus dorsi; meat quality; Pigs; Polymorphism

Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of ... more

Y. Gao; Y.H. Zhang; H. Jiang; S.Q. Xiao; S. Wang; Q. Ma; G.J. Sun; F.J. Li; Q. Deng; L.S. Dai; Z.H. Zhao; X.S. Cui; S.M. Zhang; D.F. Liu; J.B. Zhang
06/07/2011
Cassava; Gene expression; Gene walking; promoters

Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to viral and bacterial diseases prevalent in Africa. The demand ... more

M.A. Gbadegesin; J.R. Beeching
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Gene expression; Genetic variability; Isoenzymes; Ontogenetic stages

Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carried out to investigate 12 strains at five ontogenetic ... more

J.E. Georg-Kraemer; C.A.S. Ferreira; S.S. Cavalli

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