Research Article

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04/19/2011
Gene expression; Partial hepatectomy; Pit cells; Rat liver regeneration

Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high purity were obtained with Percoll ... more

C.S. Xu; X.G. Chen; C.F. Chang; G.P. Wang; W.B. Wang; L.X. Zhang; Q.S. Zhu; L. Wang
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Gene expression; In situ hybridization; Planarian; Regeneration; STAG

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those ... more

Z.Q. Yuan; B.S. Zhao; J.Y. Zhang
09/08/2010
Cacao; Gene expression; Genetic marker; Lethal factor

The lethal gene ‘Luteus-Pa’ is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of ‘Luteus-Pa’ and investigate possible lethal mechanisms. DNA samples of ... more

B.C. Rehem; A.A.F. Almeida; R.X. Corrêa; A.S. Gesteira; M.M. Yamada; R.R. Valle
05/11/2010
Dreb gene; Drought; Gene expression; Glycine max; Quantitative PCR; Water deficit

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. ... more

R. Stolf-Moreira; M.E. Medri; N. Neumaier; N.G. Lemos; R.L. Brogin; F.C. Marcelino; M.C.N. de Oliveira; J.R.B. Farias; R.V. Abdelnoor; A.L. Nepomuceno
12/11/2013
Bovine; Calcium; Gene expression; Kidney

In order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D3 ... more

L.R. Rezende; E.F. Delgado; A.R.L. Júnior; G. Gasparin; E.C. Jorge; G.B. Mourão; L.L. Coutinho
11/18/2013
Gene expression; Hepatocellular carcinoma; Laser capture microdissection; Microarray

The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. Samples were procured by LCM from HCC and patient-matched normal liver tissue surgically resected from 4 patients. RNA was isolated from the samples and reverse transcribed into cDNA. After 2-cycle linear amplification and 2 ... more

Y.M. Wei; Y.Y. Li; Y.C. Zhang; Y.Q. Nie
11/26/2013
Alcohol preference; Atf2; Candidate; Gene expression; QTL

In prior work, congenic strains carrying the DBA/2Igb (D2) region of chromosome 2 (Chr2) for alcohol preference were bred onto a C57BL/6Ibg (B6) background and as predicted were found to reduce voluntary consumption. Subsequently, interval-specific congenic recombinant strains (ISCRS) were generated and also tested. These ISCRS strains reduced the quantitative trait loci (QTL) interval to ... more

L.S. Wang; Y. Jiao; Y. Huang; X.Y. Liu; G. Gibson; B. Bennett; K.M. Hamre; D.W. Li; H.Y. Zhao; J. Gelernter; H.R. Kranzler; L.A. Farrer; L. Lu; Y.J. Wang; W.K. Gu
10/18/2013
Free amino acids; Gene expression; performance; Pigs; Protein-bound

Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus (LM) and ... more

A. Morales; F. Grageola; H. García; A. Araiza; R.T. Zijlstra; M. Cervantes
09/27/2013
Gene expression; Histone acetylation; Human testicular interstitial gene; RNF148

Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein 148 (RNF148) that is ... more

Y.Q. Liu; D.C. Tao; S.Y. Liao; Y. Yang; Y.X. Ma; S.Z. Zhang
08/21/2013
Breast muscle; Gene expression; MSTN; Pekin duck

Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning and analysis and ... more

T.S. Xu; L.H. Gu; X.H. Zhang; B.G. Ye; X.L. Liu; S.S. Hou

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