Research Article

Related GMR Articles

09/03/2012
Coffee; Gene expression; Maturation; PME; Quality

Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring ... more

S.M.B. Cação; T.F. Leite; I.G.F. Budzinski; T.Bdos Santos; M.B.S. Scholz; V. Carpentieri-Pipolo; D.S. Domingues; L.G.E. Vieira; L.F.P. Pereira
08/16/2012
Gene expression; Heat shock protein 90; heavy metal; Phascolosoma esculenta; Western blot

The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the characteristic motifs of ... more

C.H. Li; X.R. Su; M.Q. Wang; Y.Y. Li; Y. Li; J. Zhou; T.W. Li
08/06/2012
Gene expression; HSP104; Protein structure; Trypanosoma cruzi

Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a single-copy ... more

R.A. Campos; M.L. da Silva; G.V. da Costa; P.M. Bisch; J.M. Peralta; R. Silva; E. Rondinelli; T.P. Ürményi
04/03/2012
Anion exchanger; Boric acid; Gene expression; RT-PCR

Boron is an important micronutrient in plants and animals. The role of boron in living systems includes coordinated regulation of gene expression, growth and proliferation of higher plants and animals. There are several well-defined genes associated with boron transportation and tolerance in plants and these genes show close homology with human anion exchanger genes. Mutation of these ... more

F. Akbas; Z. Aydin
01/27/2012
Biomarker; Circulation; Gene expression; Ischemic stroke; MicroRNA-145; Postnatal neovascularization

Cerebral ischemia or ischemic stroke is mainly attributed to vascular and circulation disorders. Among protein biomarkers, RNA profiles have also been identified as markers of ischemic stroke. MicroRNA-145 expression is ostensibly recognized as marker and modulator of vascular smooth muscle cell phenotype; however, expression levels in ischemic stroke had not been investigated. Employing ... more

C.S. Gan; C.W. Wang; K.S. Tan
02/17/2012
Caspase-9; Dithranol; E2A; Gene expression; Human keratinocytes; Psoriasis

Although the precise causes of psoriasis remain to be elucidated, psoriasis has been known as a disorder in which factors in the immune system, enzymes and other biochemical substances that regulate skin cell division are functionally imbalanced, thereby resulting in rapid proliferation of keratinocytes and incomplete keratinization. The expression of candidate genes such as E2A and ... more

C. Ronpirin; T. Tencomnao
12/04/2014
DLK1; Gene expression; Hu sheep; IGF-I; Muscle trait

The aim of the present study was to detect delta-like 1 ho­molog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality. The diameter and density of muscle fibers and tenderness of the longissimus dorsi were measured. Growth stage, but ... more

R. Su; W. Sun; D. Li; Q.Z. Wang; X.Y. Lv; H.H. Musa; L. Chen; Y.F. Zhang; W.Z. Wu
11/27/2014
Gene expression; Housekeeping genes; Normalization; Real-time quantitative reverse transcription polymerase chain reaction; Stable expression

Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression ... more

R. Bevitori; M.B. Oliveira; M.F. Grossi-de-Sá; A.C. Lanna; R.D. da Silveira; S. Petrofeza
09/26/2014
Amino acid sequences; Expressed sequence tags; Gene expression; In silico analysis

Sweet orange (Citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. The stress tolerance could be addressed by manipulating the ethylene response factor (ERF) transcription factors because they orchestrate plant responses to environmental stress. We performed an in ... more

T.M. Ito; P.B. Polido; M.C. Rampim; G. Kaschuk; S.G.H. Souza
08/28/2014
Food availability; Gene expression; Hyriopsis cumingii; α-amylase

The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (HcAmy) was isolated from H. cumingii by the rapid ... more

G. Ren; J.Y. Tang; Y. Wang

Pages