Research Article

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02/14/2014
Gene expression; Leptin; Leptin receptor; Swine

Leptin is an important regulator of appetite, energy metabolism, and reproduction and is mainly synthesized in the adipocytes and then secreted into the bloodstream. The leptin receptor was classified as type I cytokine receptor due to its structural homology with IL-6 receptors and the signaling pathways in which they are both involved. The aim of our study is to comparatively assess the ... more

S.E. Georgescu; M.A. Manea; S. Dinescu; M. Costache
03/24/2014
Conjugated linoleic acid; Gene expression; meat quality; Pig Muscle fiber

A total of 160 Rongchang pigs (26.76 ± 1.78 kg) were randomly assigned to 5 dietary treatment groups until their body weight (BW) reached 90 kg. The diets were supplemented with 0, 0.5, 1.0, 1.5, and 2.0% conjugated linoleic acid (CLA). Our results showed that the 1.0 to 2.0% CLA-fed pigs had less back fat deposition when their BW reached 90 kg than the pigs that received less than 1% CLA ... more

J.X. Huang; R.L. Qi; X.L. Chen; X.Y. You; X.Q. Liu; F.Y. Yang; Z.H. Liu
02/14/2014
Flowering gene; Flowering locus T; Gene expression; Phalaenopsis; Spike induction

The influence of warm day and cool night conditions on induction of spikes in Phalaenopsis orchids has been studied with respect to photosynthetic efficiency, metabolic cycles and physiology. However, molecular events involved in spike emergence induced by warm day and cool night conditions are not clearly understood. We examined gene expression induced by warm day and cool night ... more

D.M. Li; F.B. Lǚ; G.F. Zhu; Y.B. Sun; Y.C. Xu; M.D. Jiang; J.W. Liu; Z. Wang
08/28/2014
Fatty acid desaturase; Gene expression; Polyunsaturated fatty acids (PUFAs); Real-time PCR; Safflower

Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and CtFAD8 in flowers. This result indicates that CtFAD3 ... more

L.L. Guan; W. Wu; B. Hu; D. Li; J.W. Chen; K. Hou; L. Wang
06/10/2014
Fertility restoration; Gene expression; Homologous cloning; Male sterility; YABBY gene family

Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration. We homologously cloned the CtYABBY1 gene of male-sterile TC1 ... more

X.L. Zhang; L.G. Zhang
05/16/2014
Enzymatic activity; Gene expression; Mangifera indica L.; p-Coumarate 3-hydroxylase; Phenylalanine ammonia lyase

Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme activity of PAL and C3H was determined in mango ... more

H. Palafox-Carlos; C.A. Contreras-Vergara; A. Muhlia-Almazán; M.A. Islas-Osuna; G.A. González-Aguilar
05/23/2014
Asterias rubens; Gene expression; High-throughput sequencing; Real-time qPCR; Reference gene; UBA52

The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas. This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression at the transcriptional level is estimated by real- ... more

A.F. Sadritdinova; A.A. Dmitriev; A.V. Snezhkina; M.S. Belenikin; G.S. Krasnov; O.G. Manylov; A.A. Kudryavtsev; N.V. Melnikova; A.S. Speranskaya; M.V. Darii; V.A. Lakunina; L.A. Uroshlev; A.O. Smurov; O.A. Stepanov; A.V. Kudryavtseva
04/14/2014
Carcass trait; Gene expression; Hu sheep; IGF-I; MyoG

In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.01 0.05) between males and females at any growth stage in ... more

W. Sun; R. Su; D. Li; H.H. Musa; Y. Kong; J.T. Ding; Y.H. Ma; L. Chen; Y.F. Zhang; W.Z. Wu
01/22/2014
5-aminolevunlinic acid; Aquaporin; Cucumber; Gene expression; NaCl stress

Aquaporins play a direct role in plant water relation under salt stress, but the effects of 5-aminolevulinic acid (ALA) on aquaporin gene expression in salt-treated plants remain unknown. This study investigated the potential effects of exogenous ALA (50 mg/dm3) on aquaporin expression levels under salt stress (75 mM NaCl) in the salt-sensitive (Jinchun No.4) and the relatively salt- ... more

F. Yan; D. Qu; Y.Y. Zhao; X.H. Hu; Z.Y. Zhao; Y. Zhang; Z.R. Zou
01/22/2014
Gene expression; Gibberellin; Oilseed rape; Reserve accumulation; Seed filling

Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA ... more

X.Q. Huang; R.Q. He; X.Y. Liao; B. Zhou; W.S. Peng; J.Z. Lin; D.Y. Tang; Y.H. Zhu; X.Y. Zhao; X.M. Liu

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