Research Article

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Y.L. Hou; X. Ding; W. Hou; B. Song; T. Wang; F. Wang; J. Li; J. Zhong; T. Xu; B.X. Ma; H.Q. Zhu; J.H. Li; J.C. Zhong
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Bioinformatic analysis; Cloning; Molecular characterization; Pseudorabies virus; UDG; UL2

A 948-bp sequence of the UL2 gene was amplified from the pseudorabies virus (PRV) Becker strain genome using polymerase chain reaction, and the gene identity was confirmed through further cloning and sequencing. Bioinformatic analysis indicated that the PRV UL2 gene encodes a putative polypeptide with 315-amino acid residues. Its encoding protein, designated UL2, has a ... more

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04/02/2013
Characterization; Cloning; Expression pattern; Spodoptera litura; Trehalase

The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and function of treh ... more

Q. Zou; P. Wei; Q. Xu; H.Z. Zheng; B. Tang; S.G. Wang
01/22/2013
Bioinformatics; Cloning; ICP22; Molecular characterization; Pseudorabies virus; US1

Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated ... more

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Capsicum annuum L.; Cloning; Cytoplasmic male sterility; NADP-isocitrate dehydrogenase; Sequence characterization

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. ... more

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C/EBPα gene; Cloning; Expression profile; Protein structure prediction; Qinchuan cattle

CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor, regulating the differentiation of adipocytes. We cloned the complete open reading frame of C/EBPα gene of Qinchuan cattle and analyzed its protein structures and expression profile in 15 tissues via DNA cloning, sequencing and RT-PCR. Analysis of the putative protein sequences revealed that C/EBPα ... more

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Actin gene; Aloe (Aloe spp); Cloning; RNA extraction

Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcription-polymerase chain reaction (RT-PCR). The result of ... more

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Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, five RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, and RXRS3, containing six domains from A to F, were cloned from ... more

Z. Li; W.Q. Wang; E.F. Zhang; G.F. Qiu
05/30/2014
Cloning; RAPD; Rose; SCAR; Sequencing

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is ... more

K.M.I. Bashir; F.S. Awan; I.A. Khan; A.I. Khan; M. Usman
03/17/2014
Bioinformatic analysis; Cloning; Molecular characterization; Pseudorabies virus; UL31

We amplified a 816-bp sequence of the UL31 gene from the pseudorabies virus (PRV) Becker strain genome. Evidence that this was the UL31 gene was confirmed by cloning and sequencing. The PRV UL31 gene encodes a putative protein of 271-amino acid residues, which was designated the UL31 protein. Bioinformatic analysis indicated that PRV UL31 contains a conserved ... more

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