Research Article

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We examined whether erythropoietin (EPO) can inhibit adipogenic differentiation of mesenchymal stem cells (MSCs) in the mouse bone marrow and its underlying mechanism. We separated and extracted mouse bone marrow MSCs and induced adipogenic differen­tiation using 3-isobutyl-1-methylxanthine, insulin, and dexamethasone. Different concentrations of EPO were added to the cells and observed ... more

G.X. Liu; J.C. Zhu; X.Y. Chen; A.Z. Zhu; C.C. Liu; Q. Lai; S.T. Chen
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Erythropoietin; Gene expression; Hypoxic adaptation; Tibetan pig

This study aimed to observe the expression characteristics of the erythropoietin (EPO) gene in different tissues of Tibetan pigs and to explore the adaptation to hypoxic environments. The cDNA in heart, liver, lung, kidney, muscle, brain, and fat of Tibetan pigs was used as the template. Through the number of cycles of the polymerase chain reaction (PCR), annealing temperature, ... more

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Hypoxia-inducible factor-1α; Keratinocytes; Nerve growth factor; Psoriasis; Vascular endothelial growth factor

Decades of research have provided the data to confirm the hypothesis that there is bidirectional communication between the central nervous system and the immune system in psoriasis pathogenesis, but the contribution of the cutaneous neural system remains underexplored. In this study, we evaluated the molecular mechanisms by which nerve growth factor (NGF) regulates hypoxia-inducible ... more

J. Zhang; W.Y. Ma
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Cardiovascular protection; Hyperlipidemia; Intermedin; Ischemia-reperfusion injury; Myocardial; Rat

Hyperlipidemia is a well-established risk factor for the development of coronary atherosclerosis, while intermedin (IMD) has been identified as a novel calcitonin/calcitonin gene-related peptide family member involved in cardiovascular protection. However, whether IMD protects against hyperlipidemia-associated myocardial ischemia/reperfusion (MI/R) injury is unknown. We established a ... more

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To investigate the effects of different doses of abnormal Savda Munziq on myocardial ischemia-reperfusion injury (MI/RI) in rats with the abnormal Savda syndrome, 50 abnormal Savda animal models were randomly divided into a control group, a model group, a high-dose group, a middle-dose group, and a low-dose group, with each group containing 10 rats. The enzyme-linked immunosorbent assay ... more

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Ischemia preconditioning; Ischemia-reperfusion injury; Liver transplantation; Rats

We explored the protective effect of ischemia preconditioning (IP) on ischemia-reperfusion injury in rat liver transplantation. An orthotopic liver transplantation model was utilized in the study. A total of 54 Sprague-Dawley rats were divided into a control group (group A, no liver transplantation), liver transplantation group (group B, heparin Ringer’s lactate solution was perfused via ... more

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Bovine; Calcium; Gene expression; Kidney

In order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D3 ... more

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Gene expression; Kidney; Promoter region; Transcription factor; WNK4

With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoal­dosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mechanism of WNK4 ... more

Z.J. Sun; Y. Li; Y.H. Li; J.S. Zhang; Q. Ding; J. Li-Ling; Y. Liang; Y.Y. Zhao
02/28/2013
Acute kidney injury; Bone marrow-derived mesenchymal stem cells; Cell differentiation; Cytokines; Erythropoietin

We investigated the effect of erythropoietin (EPO) on differentiation and secretion of bone marrow-derived mesenchymal stem cells in an acute kidney injury microenvironment. Acute kidney injury mouse models were prepared. Both renal cortices were then immediately collected to produce the ischemia/reperfusion kidney homogenate supernatant. The morphological and ultrastructural changes in ... more

N.M. Liu; J. Tian; W.W. Wang; G.F. Han; J. Cheng; J. Huang; J.Y. Zhang
06/01/2015
Follicular development; Granulosa cell; Hypoxia-inducible factor-1α; Proliferation cell nuclear antigen; Sprague-Dawley rat

Hypoxia-inducible factor-1α (HIF-1α) has been identified as a transcription factor that is involved in diverse physiological and pathological processes in the ovary. In this study, we examined whether HIF-1α is expressed in a cell- and stage-specific manner during follicular growth and development in the mammalian ovaries. Using immunohistochemistry and Western blot analysis, HIF-1α ... more

Z.H. Zhang; L.Y. Chen; F. Wang; Y.Q. Wu; J.Q. Su; X.H. Huang; Y. Cheng; Z.C. Wang

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