Research Article

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12/30/2002
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Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original host) and IAC-5 Maringá, classified as resistant ... more

A.T.S. Matsumura; A.M. Prestes; S.T. Van Der Sand; A.M.R. de Oliveira
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CTAB; DNA extraction protocol; PVP; RAPD; Sunflower

In order to investigate the mutation characteristics and to further examine the genetic variation of mutant sunflower (Helianthus annuus) obtained in plants grown from seeds exposed to space conditions, only the mature tissues such as leaf and flower could be used for DNA extraction after mutation characteristics were confirmed. The rich contents of polysaccharides, tannins ... more

J.T. Li; D.C. Chen; X.L. Zhang; Z.S. Tang; J. Yang
08/07/2007
Genetic diversity; RAPD; Triticum aestivum L.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s ... more

A. Iqbal; A.S. Khan; I.A. Khan; F.S. Awan; A. Ahmad; A.A. Khan
02/12/2007
Boll weevil; Isozymes; RAPD; Variability

Twenty-five RAPD loci and 6 isozyme loci were studied to characterize the genetic variability of natural populations of Anthonomus grandis from two agroecosystems of Brazil. The random-amplified polymorphic DNA data disclosed a polymorphism that varied from 52 to 84% and a heterozygosity of 0.189 to 0.347. The index of genetic differentiation (GST) among the six ... more

W.F.S. Martins; C.F.J. Ayres; W.A. Lucena
10/26/2006
Ganga; Hooghly; Narmada; PCR; RAPD; Tenualosa ilisha; Yamuna

RAPD was used to delineate the hilsa populations sampled from the Ganga, Yamuna, Hooghly, and Narmada Rivers at six different locations. Six degenerate primers were used to generate the fragment patterns from the samples collected. All primers were highly polymorphic and generated high numbers of amplification products. Nei’s genetic distances were calculated between locations. The ... more

M.P. Brahmane; M.K. Das; M.R. Sinha; V.V. Sugunan; A. Mukherjee; S.N. Singh; S. Prakash; P. Maurye; A. Hajra
11/01/2005
Bipolaris sorokiniana; Phytopathogen; RAPD; Variability

Bipolaris sorokiniana is a phytopathogenic fungus causing diseases of cereal crops such as common root rot, the leaf spot disease, seedling blight, and black point of the grain. Random-amplified polymorphic DNA (RAPD) assay was used to investigate the genetic diversity of 20 isolates collected from different cultivars in wheat-producing regions in Brazil. Seventy primers, with random ... more

M.Viviane Go Müller; J.Carlos Germani; S. Van Der Sand
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Bothrops moojeni; conservation genetics; Genetic structure; Genetic variability; RAPD; Vipers

Bothrops moojeni is an abundant venomous snake responsible for most of the snakebite cases in the Central region of Brazil and as a result of the anthropogenic habitat disturbance, such as the increase in extensive farming, the range of B. moojeni has been greatly fragmented. Here, we obtained genomic DNA from a total of 75 snakes belonging to four populations. Genetic ... more

N.C.L. Dutra; M.P.C. Telles; D.L. Dutra; N.J.Silva Júnior
12/12/2011
Diversity; Genetic structure; Germplasm conservation; Helianthus tuberosus; RAPD

Jerusalem artichoke (Helianthus tuberosus) is a wild relative of the cultivated sunflower (H. annuus); it is an old tuber crop that has recently received renewed interest. We used RAPD markers to characterize 147 Jerusalem artichoke accessions from nine countries. Thirty RAPD primers were screened; 13 of them detected 357 reproducible RAPD bands, of which 337 were ... more

P.P. Wangsomnuk; S. Khampa; P. Wangsomnuk; S. Jogloy; T. Mornkham; B. Ruttawat; A. Patanothai; Y.B. Fu
12/15/2011
Genotoxicity; Pseudevernia furfuracea; RAPD

We investigated the suitability and applicability of Pseudevernia furfuracea (L.) Zopf for environmental genotoxicity assessment. P. furfuracea lichen specimens were collected from 10 different Pinus species, in every 5 km, starting from around an iron-steel factory located in the central area of Karabük Province up to Yenice Forest. The impact of the pollution ... more

S. Aras; T. Beyaztaş; D. Cansaran-Duman; E. Gökçe-Gündüzer
12/20/2011
Aegiphilla sellowiana; Genetic conservation; Genetic diversity; RAPD; Riparian population; Tropical tree

The Tibagi River, located in southern Brazil, is associated with a significant degree of environmental heterogeneity, along its 550 km extension. There is concern about the integrity of this river’s ecosystem, as human interference has been increasing. Aegiphila sellowiana (Lamiaceae) is an important pioneer tree species, commonly found near rivers; the fruit is consumed by ... more

C. Medri; E.A. Ruas; C.F. Ruas; P.S. Medri; M.E. Medri; P.M. Ruas

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