Research Article

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12/12/2011
GHRH; Half-smooth tongue sole; Microsatellite polymorphism; mRNA expression; PACAP

Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences obtained from females and males consist of 4160, 4159, ... more

Q. Ma; S.F. Liu; Z.M. Zhuang; L. Lin; Z.Z. Sun; C.L. Liu; Y.Q. Su; Q.S. Tang
01/24/2013
Amino acid transporter; Isoleucine; Leucine; mRNA expression; Swine

Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b0,+ and CAT-1 in the jejunum and ... more

M. Cervantes-Ramírez; V. Mendez-Trujillo; B.A. Araiza-Piña; M.A. Barrera-Silva; D. González-Mendoza; A. Morales-Trejo
08/29/2012
Bacterial agglutination; C-type Lectin; Epinephelus akaara; Genomic structure; mRNA expression; Recombinant expression

Because of their specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate animals. Previously, only two types of collectins had been isolated from bony fish: mannan-binding lectin (MBL) and galactose-binding lectin (GalBL). We sequenced a novel collectin (designated EALec1) from the red-spotted grouper, Epinephelus ... more

Z.W. Zhang; X.F. Shi; M. Qu; S.X. Ding
12/18/2014
Chitinase-like protein; Japanese scallop Mizuhopecten yessoensis; mRNA expression; Tissue degeneration

Chitinase-like proteins (CLP) are important members of the glycoside hydrolase family 18 (GH18) and are involved in growth control and remodeling processes. In this study, a CLP transcript was isolated and sequenced from the Japanese scallop (Mizuhopecten yessoensis) after screening expressed sequence tags. The full-length complementary DNA of M. yessoensis CLP ... more

L. Gao; G.J. Xu; H. Su; X.G. Gao; Y.F. Li; X.B. Bao; W.D. Liu; C.B. He
12/04/2014
Aeromonas hydrophila; APRIL; Aquareovirus; Grass carp; mRNA expression; TWEAK

TWEAK and APRIL are important members of the TNF superfamily, which play a crucial role in several diseases. Here, we describe the identification of grass carp (Ctenopharyngodon idella) homologs of TWEAK and APRIL (designated gcTWEAK and gcAPRIL, respectively) and their response to Aeromonas hydrophila and ... more

N.P. Pandit; Y.B. Shen; Y. Chen; W.J. Wang; J.L. Li
07/04/2014
3-glucan binding protein; Gene cloning; Hard clam; Lipopolysaccharide and β-1; Meretrix meretrix; mRNA expression

Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and ... more

S.X. Liu; Z.H. Qi; J.J. Zhang; C.B. He; X.G. Gao; H.J. Li
05/29/2015
Cold shock; Embryonic development; Gene cloning; mRNA expression; Sepiella maindroni (SmYB); Y-box

Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full size of SmYB cDNA was ... more

H.P. Si; C. .L.Wang; Y.Y. Zhang; C.K. Mu; P.P. Zhan; R.H. Li; W.W. Song
05/18/2015
Heat stress; Hsp60; mRNA expression; Oncorhynchus mykiss

The enhanced expression of heat shock proteins (hsps) in organisms can be detected in response to many kinds of stressor. For fish, high temperature is an important stressor, and hsp expression is associated with differences in environmental temperature. In this study, rainbow trout (Oncorhynchus mykiss) that were accustomed to an aquatic temperature of 18°C were exposed to an ... more

H.N. Shi; Z. Liu; J.P. Zhang; Y.J. Kang; J.F. Wang; J.Q. Huang; W.M. Wang
03/27/2015
C-type lectin fold; Cloning; Metamorphosis; Mizuhopecten yessoensis; mRNA expression

C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 167 amino acids ... more

X.B. Bao; C.B. He; C.D. Fu; B. Wang; X.M. Zhao; X.G. Gao; W.D. Liu
02/06/2015
Aeromonas hydrophila; Aquareovirus; Grass carp; IL-12 p35; IL-12 p40; mRNA expression

The grass carp (Ctenopharyngodon idella) aquaculture industry in Asia is prone to bacterial and viral hemorrhagic diseases. Effective adjuvants for vaccine formulation are the need of the hour for control of these diseases and long-term sustainability of grass carp farming. In this study, the involvement of interleukin-12 (IL-12) from grass carp (gcIL‑12) in anti-bacterial and ... more

N.P. Pandit; Y.B. Shen; X.Y. Xu; H.Y. Yu; W.J. Wang; R.Q. Wang; Y.F. Xuan; J.L. Li

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