Research Article

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Breast cancer; Migration; NM23H1; Sodium valproate

Breast cancer is a common cancer in women, with a highly variable course, from inoffensive to lethal. To find a more effective strategy for its treatment, sodium valproate has been tested as an anti-cancer drug; it is the only clinically available histone deacetylase inhibitor. However, data about the effects of sodium valproate on breast cancer are insufficient in both animals and ... more

G.F. Li; T.L. Qian; G.S. Li; C.X. Yang; M. Qin; J. Huang; M. Sun; Y.Q. Han
Effective population size; Microsatellite; Migration; Nong-Han Lake; Whisker sheatfish

Nong-Han Lake, Thailand, sustains the whisker sheatfish (Micronema bleekeri Günther, 1864), which is a rare species of freshwater catfish. Wild-caught whisker sheatfish has been intensively harvested to meet market demand; yet, genetic information about this species remains unknown. To assist with the in situ conservation of whisker sheatfish populations in Nong-Han Lake ... more

P. Phongkaew; U. Arunyawat; A. Swatdipong; V. Hongtrakul
Apoptosis; Cell cycle; Gax; Migration; Proliferation; VSMCs

The Gax gene has been implicated in a variety of cell-developmental and biological processes, and aberrant Gax expression is linked to many diseases. In this study, to provide important insights for Gax-based gene therapy in vein graft restenosis and its anti-restenotic mechanism, we used rabbit vascular smooth muscle cells (VSMCs) to investigate the effects of ... more

H. Zheng; S. Xue; Z.L. Hu; J.G. Shan; W.G. Yang
Apoptosis; Curcumin; Invasion; Melanoma; Migration; Proliferation

Malignant melanoma is a melanocytic tumor with a high potential of invasion and metastasis. Curcumin is extracted from Curcuma longa L.; curcumin has anti-tumor efficacy in multiple systemic malignancies. Here, we investigated the effect of curcumin on A375 human melanoma cells. A375 cells were cultivated, passaged, and treated with different concentrations of curcumin. We ... more

Y.P. Zhang; Y.Q. Li; Y.T. Lv; J.M. Wang
Apoptosis; Gene and protein expression; MGC; shRNA; siRNA; VEGF

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the ... more

N. Ally; X.L. Zou; B.C. Jiang; L. Qin; L. Zhai; P. Xiao; H.L. Liu
Aptamer; Asthma; Nanotechnology; pRNA; siRNA; STAT5b

Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to their successful ... more

C. Qiu; W.K. Peng; F. Shi; T. Zhang
Anaphase-promoting complex; Cell cycle; Cell proliferation; siRNA

Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T cells to suppress messenger RNA ( ... more

Y.J. Shi; K.K. Huo
Apoptosis; BMPRIB; Porcine granulosa cells; siRNA; Steroidogenesis

Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs ... more

Y.Y. Zhao; X.X. Li; W. Wang; X. Chen; P. Yu; J.J. Wang; Y.X. Xu
Apoptosis; Biological behavior; Bladder cancer; Enhancer of zeste homolog 2; siRNA

We investigated the effect of siRNA targeting enhancer of EZH2 on cell proliferation, invasion, migration, and apoptosis of human bladder cancer T24 cells. An siRNA-expressing plasmid targeting the EZH2 gene was transfected into T24 cells. Quantitative polymerase chain reaction and Western blot analysis were used to detect EZH2 expression at the mRNA and protein levels, ... more

H.F. Wang; H. Yang; L.B. Hu; Y.H. Lei; Y. Qin; J. Li; C.W. Bi; J.S. Wang; Q. Huo
Chronic myeloid leukemia; Cloning expression; p15; siRNA; STI571

The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDNA3.1 vector. The K562 cell line with G418 resistance was ... more

W. Wang; Y. Du; N.N. Li; F.F. Lv; G.Q. Lin