Research Article

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08/23/2011
Combining ability; Heterosis; Line x tester analysis; Salinity; Seedlings; Tomato

We attempted to find the suitable parents for the development of tomato hybrids for high salt soils by exploiting combining ability, gene action and heterosis. Six salt-tolerant and three salt-intolerant genotypes, along with their 18 F1 crosses, were evaluated at seedling stage under 10 and 15 dS/m (NaCl) salinity stress, compared to the control level of salinity. The experiment was laid ... more

A. Saeed; M.Q. Shahid; S.A. Anjum; A.A. Khan; A. Shakeel; M.F. Saleem; N. Saeed
05/21/2013
DGGE; Estuary; Microbial community; Salinity

An estuary is a transition zone between freshwater and marine ecosystems, resulting in dilution of seawater. Estuaries are also considered environments of intense biological activity related to the processes of nutrient cycling. The aim of this study was to evaluate the microbial community composition along a salinity gradient in the estuary of the Cachoeira River, located in southern ... more

C.S.P. Rodrigues; S.S. Souza; R.P. Rezende; A. Silva; J.L. Andrioli; H. Costa; R. Fontana; J.C.T. Dias
06/13/2013
Goat; MicroRNA characteristics; Real-time quantitative PCR; Solexa deep-sequencing technology

MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this study, small RNA cDNA libraries of ... more

Y.H. Ling; J.P. Ding; X.D. Zhang; L.J. Wang; Y.H. Zhang; Y.S. Li; Z.J. Zhang; X.R. Zhang
10/31/2014
Clone; Differential expression; Invariant chain isoform; Muscovy duck; Real-time quantitative PCR

Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for ... more

S.J. Liu; F.F. Chen; C. Wu; Q.S. Ni; W.Y. Yu
06/11/2015
DNA extraction; Half-seed; Oryza sativa L.; Polymerase chain reaction; Pre-germination genotyping

Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming ... more

Y.B. Duan; F.L. Zhao; H.D. Chen; H. Li; D.H. Ni; P.C. Wei; W. Sheng; J.T. Teng; A.M. Zhang; J.P. Xue
08/24/2012
E3 ligase; Escherichia coli; Maltose; Proteosome; Ubiquitin

SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional studies. We produced and ... more

A. Gholizadeh