Research Article

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Alternative splicing; SAGE; Tumorigenesis

Although alternative splicing of many genes has been found associated with different stages of tumorigenesis and splicing variants have been characterized as tumor markers, it is still not known whether these examples are sporadic or whether there is a broader association between the two phenomena. In this report we evaluated, through a bioinformatics approach, the expression of ... more

N. Kirschbaum-Slager; G.M.P. Lopes; P.A.F. Galante; G.J. Riggins; S.J. de Souza
Alternative splicing; Dairy cattle; Lactoferrin gene; Mastitis

Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the splice variants of the bLF gene in the mammary ... more

J.M. Huang; Z.Y. Wang; Z.H. Ju; C.F. Wang; Q.L. Li; T. Sun; Q.L. Hou; S.Q. Hang; M.H. Hou; J.F. Zhong
Alternative splicing; Bull; Cow; Liver; PEPCK gene; Testis

Phosphoenolpyruvate carboxykinase 1 (PCK1), also named PEPCK-C, is a multiple-function gene that is involved in gluconeogenesis, glyceroneogenesis, reproduction, female fertility, and development of obesity and diabetes. How its many functions are regulated was largely unknown. Therefore, we investigated mRNA expression and possible splice variants of PCK1 ... more

Z.B. Zhang; W. Zhang; R.L. Li; J.B. Li; J.F. Zhong; Z.S. Zhao; J.M. Huang
Alternative splicing; Cancer-testis gene; Cloning; ODF3; ODF4

The outer dense fiber (ODF) genes encode proteins that co-assemble along the axoneme of the sperm tail. Recently, it was demonstrated that some ODF genes are aberrantly expressed in tumors, including prostate adenocarcinoma, basal cell carcinoma, and chronic myeloid lymphoma. We cloned ODF3 and ODF4 cDNA from the testis of a patient suffering from ... more

S. Ghafouri-Fard; S. Ghafouri-Fard; M.H. Modarressi
Alternative splicing; C4A gene; Dairy cattle; Mastitis; Transcription pattern

The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and ... more

Y. Yang; J.M. Huang; Z.H. Ju; Q.L. Li; L. Zhou; R.L. Li; J.B. Li; F.X. Shi; J.F. Zhong; C.F. Wang
Alternative splicing; deletion; Dephosphorylation; GluR-B minigene; NSSR1

Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat shock and K252a treatment increase the truncated splicing isoform ... more

W. Zhang; L.L. Li; X.H. Chen; Z.Y. Peng
Alternative splicing; Bioinformatics; Exon skipping; Expressed sequences; Extracellular matrix; Human transcriptome

Alternative splicing increases protein diversity through the generation of different mRNA molecules from the same gene. Although alternative splicing seems to be a widespread phenomenon in the human transcriptome, it is possible that different subgroups of genes present different patterns, related to their biological roles. Analysis of a subgroup may enhance common features of its ... more

N.Jo Sakabe; M.Dulcetti Vibranovski; S.José de Souza
Alternative splicing; Heteroduplex; methodology; S1 nuclease

The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of base pairing involving exons common between the two variants. S1 ... more

E.N. Ferreira; M.C.R. Rangel; P.B. Pineda; D.O. Vidal; A.A. Camargo; S.J. Souza; D.M. Carraro
Carcass traits; Cattle; Heritability; Slaughter end points

We present estimates of heritability for carcass traits of cattle published in the scientific literature. Seventy-two papers published from 1962 to 2004, which reported estimates of heritability for carcass traits, were reviewed. The unweighted means of estimates of heritability for 14 carcass traits by slaughter end point (age, weight, and fat depth) were calculated. Among the three end ... more

A.Ríos Utrera; L.Dale Van Vleck
Blood coagulation; Carrier DNA sample; Cattle; Factor XI; Recessive DNA sample

An insertion mutation within exon 12 of the factor XI gene has been described in Holstein cattle. This has opened the prospect for large-scale screening of cattle using the polymerase chain reaction (PCR) technique for the rapid identification of heterozygous animals. To facilitate such a screening process, the mutant and normal alleles of factor XI gene, represented by 244- and 320-bp ... more

P.N. Mukhopadhyaya; M. Jha; P. Muraleedharan; R.R. Gupta; R.N. Rathod; H.H. Mehta; V.K. Khoda