Research Article

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12/01/2009
Cervical cancer; Genotyping; Human papillomavirus; Prophylactic vaccines

Human papillomavirus (HPV) has been extensively studied concerning genomic structure, infection mechanisms, and diversity of types, as well as disease progression stages and development of vaccines. HPV type prevalence can differ in specific populations in different countries, according to ethnicity. This is the first report of an integrated project to evaluate the incidence of HPV types ... more

M.F.P.T.Baldez da Silva; B.S. Chagas; V. Guimarães; L.M.C. Katz; P.M. Felix; P.M. Miranda; A.A. Lima; L.C. Arraes; D.B.G. Martins; J.L.Lima Filho; R.C. Stocco; S. Crovella; A.C. Freitas; W. Beçak
08/18/2009
Candidate gene; Genotyping; PCR-RFLP; POU1F

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were ... more

A.C.P. Silveira; T.F. Braga; J.F. Almeida; R.C. Antunes; P.F.A. Freitas; A.S.M. César; A.S.M. César; E.C. Guimarães
10/19/2011
Genotyping; Heterozygote deficiency; Microsatellite; Null alleles; Parentage; White-tailed deer

Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs. We used empirical ... more

A.C. Kelly; N.E. Mateus-Pinilla; M. Douglas; M. Douglas; P. Shelton; J. Novakofski
11/03/2010
Diversity; Genotyping; Prunus; Rootstock; SSR

Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of ... more

Z. Turkoglu; S. Bilgener; S. Ercisli; M. Bakır; M. Bakır; A. Koc; M. Akbulut; R. Gercekcioglu; M. Gunes; A. Esitken
11/07/2013
Chrysanthemum; Genotyping; Marker development; Population structure; SSR

Chrysanthemums are well known for their esthetic and medicinal values. Characterization of chrysanthemums is vital for their conservation and management as well as for understanding their genetic relationships. We found 12 simple sequence repeat markers (SSRs) of 100 designed primers to be polymorphic. These novel SSR markers were used to evaluate 95 accessions of chrysanthemums (3 ... more

A.A. Khaing; K.T. Moe; W.J. Hong; C.S. Park; K.H. Yeon; H.S. Park; D.C. Kim; B.J. Choi; J.Y. Jung; S.C. Chae; K.M. Lee; Y.J. Park
08/12/2013
Genotyping; Haplotype analysis; IL-10 gene promoter; Single nucleotide polymorphism

The purpose of this study was to determine whether interleukin-10 (IL-10) promoter polymorphisms are associated with leprosy or their subtypes in ethnic groups from southwest China. Genotyping using TaqMan® SNP Genotyping Master Mix and ABI 7500 real-time PCR system was performed for IL-10 T3575A, G2849A, C2763A, A1082G, C819T, and C592A in 189 healthy controls (40 ± 18 years) and 193 ... more

X.H. Chen; J.H. Xiong; Y. Ning; Y. Wen; J. Liu; C. Mao; X.M. Weng; J.C. Gu
04/25/2013
F. culmorum; F. graminearum; Fusarium head blight; Genotyping; Random-amplified polymorphic DNA

Five Fusarium graminearum and 12 F. culmorum isolates, primarily pathogenic species of Fusarium head blight, were obtained from naturally infected wheat from various agro-ecological regions of Turkey. Genotyping of the isolates was carried out using random-amplified polymorphic DNA (RAPD). Sixty-five 10-mer oligonucleotide primers were used to amplify the RAPD ... more

E. Yörük; G. Albayrak
12/19/2012
Dendrogram; Genotyping; Mangifera indica; Variability

Allelic patterns and genetic distances were examined in a collection of 103 foreign and Brazilian mango (Mangifera indica) accessions in order to develop a reference database to support cultivar protection and breeding programs. An UPGMA dendrogram was generated using Jaccard’s coefficients from a distance matrix based on 50 alleles of 12 microsatellite loci. The base pair number ... more

I.C.Ndos Santos Ribeiro; F.P.Lima Neto; C.A.F. Santos
06/21/2012
ARMS-PCR; Genotyping; mutations; PCR-SSCP; PRNP alleles; Sheep

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from ... more

C.L. Zhao; R. Wu; L. Liu; F.D. Li; X.L. Zhang; C. Wang; F. Wang; X.L. Diao; H.W. Guan; X. Wang; L. Zhou
05/21/2012
Genotyping; Germplasm; Molecular markers; Simple sequence repeats; Tvv1; Vitis spp

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiate grapevine accessions with just one pair of primers. In order ... more

G.C. Sant’Ana; J.L. Ferreira; H.S. Rocha; A. Borém; M. Pasqual; G.M.A. Cançado

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