Research Article

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AFLP; Megalobrama amblycephala; SCAR; Sex-specific marker

Sex-specific DNA markers are useful for studying sex-determination mechanisms and establishment of monosex populations. Three widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in China) of blunt snout bream (Megalobrama amblycephala) were screened with AFLPs to search for sex-linked markers. Female and male pools (10 individuals in each pool) from each ... more

H.O. Rao; J.C. Deng; W.M. Wang; Z.X. Gao
04/10/2015
Expression pattern; Gill; HSP90; Megalobrama amblycephala; Nitrite exposure

Heat shock protein 90 (HSP90), a highly conserved and multi-functional molecular chaperone, plays an essential role in cellular metabolism and stress response. In this study, HSP90 cDNA named MaHSP90 was cloned from Wuchang bream (Megalobrama amblycephala) gills by using rapid amplification of cDNA ends. The full-length MaHSP90 cDNA is 2674 bp and consists of a 3',5'-untranslated ... more

S.M. Sun; J. Zhu; X.P. Ge; C.F. Zhang; L.H. Miao; X.J. Jiang
11/03/2016
Alpha-actinin 3; Angiotensin-converting enzyme; Muscle fiber; Polymorphism; Scoliosis

The etiology of adolescent idiopathic scoliosis remains unknown. Angiotensin-converting enzyme and α-actinin-3 polymorphisms influence the characteristics of muscle fibers. The aim of this study was to examine the association between idiopathic scoliosis and genetic polymorphism of angiotensin-converting enzyme and α-actinin-3. Ninety-seven females with scoliosis, ... more

R.P. Luciano; M. Wajchenberg; S.S. Almeida; C.E.N. Amorim; L.M.R. Rodrigues; R.C. Araujo; E.B. Puertas; F. Faloppa; R.P. Luciano; M. Wajchenberg; S.S. Almeida; C.E.N. Amorim; L.M.R. Rodrigues; R.C. Araujo; E.B. Puertas; F. Faloppa
09/28/2015
Gene expression; Hypoxia; Muscle; Pig

Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression level of four hypoxia-related genes ( ... more

J. Zhang; L. Chen; K.R. Long; Z.P. Mu
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Annual Medicago; Gene expression; Microarray analysis; Salinity

The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive ... more

F. Gharaghani; F. Rafiei; N. Mirakhorli; E. Ebrahimie
08/19/2015
Amino acid transporter; Branched-chain amino acid; Gene expression; Pigs

Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b0,+AT and CAT1) and neutral (B0AT1) AA transporters in the small intestine, liver, and skeletal ... more

M. Cervantes; N. Arce; H. García; M. Cota; J.K. Htoo; A. Morales
12/28/2015
Carotenoid; Citrus; Gene expression; Stability

Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome and gene ... more

T.T. Pinheiro; D.S. Nishimura; F.B. De Nadai; A. Figueira; R.R. Latado
12/21/2015
BP neural network; Colon cancer; Gene expression; S-Kohonen neural network; SVM neural network

Based on gene expression, we have classified 53 colon cancer patients with UICC II into two groups: relapse and no relapse. Samples were taken from each patient, and gene information was extracted. Of the 53 samples examined, 500 genes were considered proper through analyses by S-Kohonen, BP, and SVM neural networks. Classification accuracy obtained by S-Kohonen neural network reaches 91 ... more

H.P. Hu; Z.J. Niu; Y.P. Bai; X.H. Tan
12/15/2015
Bovine mammary epithelial cells; Deep sequencing; Escherichia coli; Gene expression; Klebsiella pneumoniae; Staphylococcus aureus

The goal of this study was to characterize the transcriptome of primary bovine mammalian epithelial cells (pBMECs) and to identify candidate genes for response and resistance to Staphylococcus aureus (strain S108), Escherichia coli (strain E23), and Klebsiella pneumoniae (strain K96) infection. Using Solexa sequencing, approximately 4.9 million total sequence tags ... more

L. Xiu; Y.B. Fu; Y. Deng; X.J. Shi; Z.Y. Bian; A. Ruhan; X. Wang
11/30/2015
Barley; Drought stress; Gene cloning; Gene expression; HbSINA4; Hordeum vulgare

Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety ‘Himalaya 10’ and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of HbSINA4 was 1052 bp long, including an open reading frame of 771 ... more

H.J. Yuan; X.M. Luo; T.S. Nyima; Y.L. Wang; Q.J. Xu; X.Q. Zeng

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