Research Article

Related GMR Articles

Enriched library; Hybrid; Polyploid; SSRs

Fifteen microsatellite loci were identified in the tetraploid spined loach, Cobitis biwae (Teleostei: Cobitidae). Among these, 14 were polymorphic (5-31 alleles) and showed moderate to high cross-species amplification transferability in four related species, Cobitis matsubarai, Cobitis taenia, Misgurnus anguillicaudatus, and Misgurnus fossilis. ... more

O. Jablonska; A. Marín; K. Kowalewska; T. Fujimoto; K. Arai; O. Jablonska; A. Marín; K. Kowalewska; T. Fujimoto; K. Arai
Genetic dissimilarity; Genetic purity; Microsatellite markers; Mixture of cultivars; Phaseolus vulgaris; Simple sequence repeat

The aim of this study was to evaluate genetic divergence in the ‘Carioca’ (beige with brown stripes) common bean cultivar used by different institutions and in 16 other common bean cultivars used in the Rede Cooperativa de Pesquisa de Feijão (Cooperative Network of Common Bean Research), by using simple sequence repeats associated with agronomic traits that are highly distributed in the common ... more

J.S. Veloso; W. Silva; L.R. Pinheiro; J.Bdos Santos; N.S. Fonseca; M.P. Euzebio
Aquaculture; Golden mandarin fish; Microsatellite markers; Selective breeding

In this study, 12 polymorphic microsatellites were inves­tigated to determine the genetic diversity and structure of 5 consecu­tive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breeding populations was ... more

X.N. Luo; M. Yang; X.F. Liang; K. Jin; L.Y. Lv; C.X. Tian; Y.C. Yuan; J. Sun
Microsatellite markers; Molecular marker-assisted breeding; Osmanthus fragrans; Roche 454 sequencing

Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design were identified ... more

W.J. Yuan; Y. Li; Y.F. Ma; Y.J. Han; F.D. Shang
Bagarius yarrelli; Catfish; Microsatellite markers; RNA-seq

The yellow sisorid catfish (Bagarius yarrelli) is a vulnerable fish species. In this study, seven polymorphic microsatellite DNA markers for yellow sisorid catfish were described, using RNA-Seq methodology. In B. yarrelli (N = 44) from a Hekou wild population, allelic frequency, and observed and expected heterozygosities per locus varied from two to six, 0.0333 to 0.6793 ... more

M. Du; Y.H. Liu; B.Z. Niu
Fenneropenaeus penicillatus; FIASCO protocol; Microsatellite markers

Until recently, Fenneropenaeus penicillatus was considered a commercial shrimp species. However, in 2005, it was included on the Red List as an endangered species by the Chinese government. In this study, 19 new microsatellite markers in F. penicillatus were developed and tested in samples of 32 wild individuals from Nanao, China. Twelve loci were polymorphic and 7 were ... more

Y. Yuan; J.B. Shangguan; Z.B. Li; Y.F. Ning; Y.S. Huang; B.B. Li; X.Q. Mao
Bellamya; Genetic diversity; Microsatellite markers; Population genetics

Bellamya is a widely distributed freshwater snail genus in China; however, its genetic diversity is completely unknown. Sixty-five novel microsatellite loci were isolated and characterized from a microsatellite-enriched library of Bellamya aeruginosa genomic DNA. Most of the 65 loci were successfully amplified. We found high polymorphic information content values for these loci, ... more

Q.H. Gu; Q.Q. Cheng; X.J. Li; C.J. Zhou
Genetic variability; Microsatellite markers; Population structure; Tunisian indigenous sheep

The genetic diversity and genetic relationship of the two main groups of African sheep, thin-tailed and fat-tailed sheep, represented by the indigenous Tunisian sheep breeds “Barbarine” (BAR, fat-tailed) and “Queue Fine de l’Ouest” (QFO, thin-tailed) were investigated. The genotypes of 110 animals belonging to these two breeds and their crossbreed (CRO) were assessed using 17 ... more

Y. Ben Sassi-Zaidy; F. Maretto; F. Charfi-Cheikhrouha; A. Mohamed-Brahmi; M. Cassandro; Y. Ben Sassi-Zaidy; F. Maretto; F. Charfi-Cheikhrouha; A. Mohamed-Brahmi; M. Cassandro
Genetic dissimilarity; Genetic divergence; Microsatellite markers; Seedlings; Ward Method
Brazil nut tree is a species of economic importance for
the Amazon region, known for the commercialization of its almonds.
The objective of this work was to study the genetic diversity among halfsib
progenies from different Brazil nut trees present in native forest in
the municipality of Itaúba, MT, belonging to the Brazilian Amazon. In a
... more
L.D. Giustina; A.B. Baldoni; F.D. Tardin; F.S. Gregolin; H. Tonini; L.G. Neves; L.P. Ribeiro; P.E. Teodoro
Brazil nut; Brazilian Amazon; conservation genetics; Microsatellite markers; Tropical tree

Knowing the mating patterns is important to determine the number of trees necessary for seed collection for conservation ex situ, tree breeding and environmental reforestation purposes. We investigated B. excelsa individuals and fruits, to check mating system index variations in a population by using open-pollinated seeds which were hierarchically sampled within ... more

L.D. Giustina; A.B. Baldoni; H. Tonini; V.C.R. Azevedo; L.G. Neves; F.D. Tardin; A.M. Sebbenn