Research Article

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Bleomycin; Idiopathic pulmonary fibrosis; Lung fibrosis; Toll-like receptor

The specific role of Toll-like receptor 4 (TLR4) in bleomycin-induced lung fibrosis of mice, a model of human idiopathic pulmonary fibrosis, has not been characterized. We injected bleomycin intratracheally into TLR4 knockout (TLR4-/-) and wild-type (WT) mice. Twenty-one days after injection, mice were sacrificed and their lungs were harvested for pathological, hydroxyproline, ... more

X.X. Li; D.Y. Jiang; X.X. Huang; S.L. Guo; W. Yuan; H.P. Dai
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Bladder cancer; Gene polymorphism; PCR-RFLP; Toll-like receptor

In this study, the association between the 729G/C polymorphism in Toll-like receptor 4 (TLR4) and the risk of bladder cancer was investigated. A total of 376 patients with bladder cancer and 380 healthy volunteers from the Third Xiangya Hospital of Central South University (China) were enrolled in this study between January 2008 and February 2014. The TLR4-729G/C polymorphism was ... more

B. Wan; J. Tan; Q. Zeng; L.Y. He; Y. Gan; Y.B. Dai; K. Yao
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Hepatic cirrhosis; Hepatocellular carcinoma; Innate immunity; Toll-like receptor

Toll-like receptors (TLRs) can specifically identify pathogen-associated molecular patterns (PAMPs) by recognizing structural patterns in diverse microbial molecules, and can provide an effective defense against multiple microbial infectious. A variety of TLRs can be expressed on the surface of liver parenchymal as well as nonparenchymal cells. Kupffer cells are a type of hepatic ... more

L. Sun; J.J. Dai; W.F. Hu; J. Wang; L. Sun; J.J. Dai; W.F. Hu; J. Wang
04/28/2016
Fluid resuscitation; Hemorrhagic shock; MDSCs; Myeloid-derived suppressor cells; Rats

We investigated the influence of different fluid resuscitation techniques on the number of myeloid-derived suppressor cells (MDSCs) in rats. Seventy-two healthy Sprague-Dawley rats were randomly divided into groups that received sham operation (Sham group), hypertonic saline (HRS group), lactated ringer’s solution (LRS group), or crystalloid solution (LCRS group). Six rats from each ... more

Z.J. Wang; H.X. Wang; L. Li; L. Wang; H.H. Dou; Z.J. Wang; H.X. Wang; L. Li; L. Wang; H.H. Dou
09/16/2016
Downstream target genes; Injury repair; miR-1; miR-206; miRNAs; Rats

The effects of muscle-specific miRNAs in the repair of exercise-induced muscle injury were investigated by examining the changes in their expression and that of the target genes in rat skeletal muscle. Two-month-old agile male rats were randomly divided into exercise and static control groups, the former subdivided into 0-h, 6-h, 12-h, 1-day, 2-day, 3-day, 1-week, and 2-week groups based ... more

W. Wu; W. Wu
12/02/2016
Administration, Oral; Animals; Bone marrow cells; Cell proliferation; Cells, Cultured; Guatemala; Humans; Lymphocytes; male; Mexico; Micronucleus tests; Mutagenicity Tests; Plant Extracts; Rats; Rubus; Sister chromatid exchange; Toxicity Tests, Subchronic

Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of ... more

S. González-Hernández; D. González-Ramírez; M.I. Dávila-Rodríguez; A. Jimenez-Arellanez; M. Meckes-Fischer; S. Said-Fernández; E.I. Cortés-Gutiérrez
12/02/2016
Animals; Citalopram; Dementia, Vascular; Disease Models, Animal; Dopamine; Gene Expression Regulation; Hippocampus; Humans; Injections, Intraperitoneal; male; Neurons; Norepinephrine; Random Allocation; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT1A; Selective Serotonin Reuptake Inhibitors; Serotonin; Treatment outcome

5-hydroxytryptamine receptor 1A (5-HT1AR) is closely associated with cognitive functions. Selective serotonin reuptake inhibitors (SSRIs) can protect individuals from brain damage following ischemia/hypoxia. To investigate the function of SSRIs in vascular dementia (VD), we established a rat model of VD, and observed the effect of SSRIs on the expression of 5-HT1AR mRNA and neurotransmitters. ... more

K. Guo; G. Yin; X.H. Zi; H.X. Zhu; Q. Pan
12/19/2016
Animals; Antimutagenic Agents; Antioxidants; Chromosome aberrations; Cyclophosphamide; Fruit; male; Malpighiaceae; Models, Animal; Plant Extracts; Rats; Rats, Wistar

Barbados cherry (BC) (Malpighia glabra Linnaeus) is a functional fruit that is consumed to prevent disease. It is used as an adjuvant in the treatment of several diseases, and acts as an antianemic, an appetite stimulant, a wound healer, an anti-inflammatory, a mineralizer, an antifungal, and an antioxidant agent. Several chemotherapeutic agents, such as cyclophosphamide, may result in ... more

E. Düsman; I.V. Almeida; L.T.D. Tonin; V.E.P. Vicentini
12/19/2016
Animals; Antimutagenic Agents; Cell line; Cell proliferation; DNA damage; Dose-Response Relationship, Drug; Plant Extracts; Rats; Vanillic Acid

The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used ... more

I.V. Almeida; F.M.L. Cavalcante; V.E.P. Vicentini
1/23/2017
Adipose tissue; Animals; Cell differentiation; Cell proliferation; Estradiol; Female; Pregnancy; Progesterone; Rats; Stem cells

Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem cells (ADSCs), which are a type of adult mesenchymal stem cells, can ... more

J.N. Li; Y. Zhang; Y.F. Wang; J.Y. Chen

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