Research Article

Related GMR Articles

Fluorescent dye; Gene rearrangement; Hematological malignancies; IgH gene; Real-time quantitative PCR; TCRγ gene

The aim of our study was to investigate the association of IgH and TCRγ gene rearrangements in hematological malignancies with the disease and clinical application. IgH and TCRγ gene rearrangements were determined in 69 paraffin and bone marrow specimens with SYBR Green I fluorescent dye and RQ-PCR method, including 21 paraffin-embedded tissues of the onset cases and 48 bone marrow ... more

J. Yao; R.H. Wang; B. Li; Y. Wang; P. Zhu; X.J. Li; X.Q. Ma; M.J. Ma; L.F. Yin
Gene expression; IGF1R; Pyometra; Real-time quantitative PCR; Reference genes

Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by quantitative measurements of mRNA levels ... more

M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma; M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma
Betula kirghisorum; Expression pattern; GRAS transcription factor; NaHCO3 stress; Subcellular localization

GRAS proteins are plant-specific transcription factors that are involved in the regulation of root and shoot growth. Here, we cloned BkGRAS2 from Betula kirghisorum (abbreviated to Bk) and analyzed the physicochemical properties and expression pattern of the encoded protein. BkGRAS2 had an open reading frame of 1614 bp encoding 537 amino acid residues. The deduced ... more

G.Y. Li; C.J. Yang; G.J. Liu
Bioinformatic analysis; Expression pattern; Fibroblast growth factor 5; Inner Mongolia Cashmere goat

Fibroblast growth factor 5 (FGF5) is a secreted signaling protein that belongs to the FGF family, and was found to be associated with hair growth in humans and other animals. The Inner Mongolia Cashmere goat (Capra hircus) is a goat breed that provides superior cashmere; this breed was formed by spontaneous mutation in China. Here, we report the cloning, molecular characterization, ... more

W.L. Bao; R.Y. Yao; Q. He; Z.X. Guo; C. Bao; Y.F. Wang; Z.G. Wang
Chemoreception; Expression pattern; Rhynchophorus ferrugineus; Transcriptome

Olfaction is crucial for insects’ survival because it enables them to recognize various environmental information. It is primarily mediated by a large family of chemoreceptors, including olfactory receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs). Here, we assembled the transcriptome of the economically important pest of palms, Rhynchophorus ferrugineus, to ... more

W. Yan; L. Liu; W.Q. Qin; C.X. Li; Z.Q. Peng
Banna mini-pig inbred line; Expression pattern; Gene structure; Phylogenetic analysis; Splice isoform; Testis-specific serine kinase

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, ... more

P. Wang; H.L. Huo; S.Y. Wang; Y.W. Miao; Y.Y. Zhang; Q.L. Zhang; F.Q. Li; L.X. Liu; W.Z. Li; Y.Z. Zeng; J.L. Huo; H. Xiao
Expression pattern; Flower bud; Male sterility; miRNA

MicroRNAs (miRNAs) are a class of non-coding endogenous negative regulators that regulate gene expression at both the transcriptional and post-transcriptional levels. However, little is known about the expression characteristics of miRNAs during pollen development in Brassica oleracea. In this study, five known and three novel miRNAs were identified and their expression patterns were ... more

J.H. Song; J. Yang; F. Pan; B. Jin
Crustacean; Expression pattern; Feminization-1 homolog b (Fem1b); Macrobrachium nipponense; Post-larval; RT-qPCR

Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. ... more

N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu; N.M.A. Rahman; H. Fu; H. Qiao; S. Jin; H. Bai; W. Zhang; F.W. Jiang; G. Liang; S. Sun; Y. Gong; F.F. Jiang; Y. Xiong; Y. Wu