Research Article

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07/14/2016
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Heterosis, or enhancement through outbreeding, is one of the most promising approaches for increasing crop yield. Male sterility (MS), which promotes heterosis, has been widely applied in hybrid crop production. Medicago truncatula is a model legume species and is closely related to M. sativa, an important legume forage plant. Although the molecular mechanisms of MS in ... more

L.H. Shao; X.W. Zheng; D.X. Yi; C. Li
07/14/2016
Genetic analysis; Male sterility; Medicago truncatula; Tapetum

Heterosis, or enhancement through outbreeding, is one of the most promising approaches for increasing crop yield. Male sterility (MS), which promotes heterosis, has been widely applied in hybrid crop production. Medicago truncatula is a model legume species and is closely related to M. sativa, an important legume forage plant. Although the molecular mechanisms of MS in ... more

L.H. Shao; X.W. Zheng; D.X. Yi; C. Li; L.H. Shao; X.W. Zheng; D.X. Yi; C. Li
10/05/2015
Expression patterns; GhRAXs; Shoot branching; Gossypium hirsutum L.; RACE

The formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. Several transcription factor-encoding genes, including CUC3, RAX, LAS, LOF1, and ROX, have been cloned by screening for axillary meristem mutants in Arabidopsis thaliana. These genes ... more

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Curcuma wenyujin; Genetic diversity; Inter simple sequence repeat (ISSR); Phylogenetic analysis; Population; Random amplified polymorphic DNA (RAPD)

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of ... more

W.H. Zheng; Y. Zhuo; L. Liang; W.Y. Ding; L.Y. Liang; X.F. Wang
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16S rRNA gene; Agronomic crops; Bioprospection; Biotechnology; Endophytic bacteria; Phylogenetic analysis

Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size, and availability of databases ... more

C. Bredow; J.L. Azevedo; J.A. Pamphile; C.A. Mangolin; S.A. Rhoden
07/28/2015
Phylogenetic analysis; Rhoptry protein 20; Sequence variation; Toxoplasma gondii; Toxoplasmosis

Toxoplasma gondii, an opportunistic protozoan parasite, infects almost all warm-blooded animals. In this study, we examined the sequence variation in rhoptry protein 20 (ROP20) genes among 18 T. gondii isolates collected from different hosts and geographical regions. Full length ROP20 genes were amplified and sequenced. The results showed that the genes were ... more

H.R. Ning; J. .L.Wang; S.Y. Qin; S.Y. Huang; Z.L. Lou; L.Y. Hu; X.Q. Zhu
12/08/2015
Bitter taste receptor; Forest musk deer; Phylogenetic analysis; Structure analysis; T2R16

Sensing bitter tastes is crucial for most animals because it can prevent them from ingesting harmful food. This process is mainly mediated by the bitter taste receptors (T2R) that are largely expressed in the taste buds. Previous studies have identified some T2R gene repertoires. Marked variation in repertoire size has been noted among species. However, research on T2Rs is ... more

G.J. Zhao; N. Wu; D.Y. Li; D.J. Zeng; Q. Chen; L. Lu; X.L. Feng; C.L. Zhang; C.L. Zheng; H. Jie
11/23/2015
Banna mini-pig inbred line; Expression pattern; Gene structure; Phylogenetic analysis; Splice isoform; Testis-specific serine kinase

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, ... more

P. Wang; H.L. Huo; S.Y. Wang; Y.W. Miao; Y.Y. Zhang; Q.L. Zhang; F.Q. Li; L.X. Liu; W.Z. Li; Y.Z. Zeng; J.L. Huo; H. Xiao
11/26/2015
Gene expression pattern analysis; Gene structure; GRAS gene family; Phylogenetic analysis; Protein motif analysis; Tobacco

Members of the GRAS gene family are important transcriptional regulators. In this study, 21 GRAS genes were identified from tobacco, and were classified into eight subgroups according to the classification of Arabidopsis thaliana. Here, we provide a preliminary overview of this gene family in tobacco, describing the gene structure, gene expression, protein motif ... more

Y.Q. Chen; S.S. Tai; D.W. Wang; A.M. Ding; T.T. Sun; W.F. Wang; Y.H. Sun
11/23/2015
Chloroplast genome; Conserved sequence; Phylogenetic analysis; species identification; Universal primer; Variable sequence

Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. To identify conserved regions that can be used to design primers and amplify variable sequences for use in plant phylogenetic studies, the ... more

Y.C. Yang; T.L. Kung; C.Y. Hu; S.F. Lin

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