Research Article

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Baculovirus; BmMNP; BmMNPV; ORF10; ORF14 Bombyx mori multiple nucleopolyhedrovirus subgroup

ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) were amplified, cloned and sequenced. Nucleotide analysis of these genes and those of other baculoviruses showed that these genes are highly conserved. The p10 protein from BmMNPV ORF10 has 70 amino acid residues similar to that of the four other known BmNPV strains. The BmMNPV ORF14 alignment showed a higher ... more

S.A. Santos; J.L.C. Silva; V.A. Balani; F.A.V. Seixas; M.A. Fernandez
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Baculovirus; Cryptonephric system; Cytopathology; Lepidoptera; Viral polyhedra

Bombyx mori is an insect whose cocoon is used in the sericulture industry, which is an important activity in parts of southern Brazil. When parasitized by Bombyx mori nucleopolyhedrovirus (BmNPV) of the genus Alphabaculovirus (alphaBV), it develops nuclear polyhedrosis disease. In Brazil, an alphaBV was isolated from larvae of B. mori and various target ... more

S.A. Vessaro-Silva; R.M.C. Brancalhão; M.P.D. Baggio; L.F.C. Ribeiro
04/03/2014
Baculovirus; Hindgut; Insect; Lepidoptera

Alphabaculovirus is an entomopathogenic virus genus that infects Bombyx mori, which is known as the Bombyx mori multiple nucleopolyedrovirus (BmMNPV). This virus is polyorganotrophic, and a series of tissues are known as targets; however, there is currently no information regarding infection in the pylorus, the segment of the hindgut that is present in the midgut ... more

M.P.D. Baggio; L.F.C. Ribeiro; S.A. Vessaro-Silva; R.M.C. Brancalhão
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Baculovirus; Bombyx mori L; Classical swine fever virus (CSFV); E2 glycoprotein; Insect cell line

The envelope E2 glycoprotein of classical swine fever virus (CSFV) is an immunodominant protein which neutralizes the virus. Previous trials to eradicate CSFV have been expensive, inefficient and time-consuming process without complete success. In contrast to DNA transfection into cultured cells, the efficacy of gene transduction in in vivo organ is very ... more

X. Hu; Y. Chen; C.O. Singh; S. Zhang; Y.G. Miao; Y. Zhou; J. Xu; B. Roy
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; Larval integument cells; Baculovirus; Bombyx mori; Cuticle; Cytopathology; Ultrastructure

Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus of the family Baculoviridae that causes disease in the silkworm, Bombyx mori, leading to a series of metabolic disorders that severely affect silk production. The main external sign of BmNPV infection can be observed in the integument of infected caterpillars, which is an ... more

A.L. Oro; R.M. Costa; E.A. Britta; C.V. Nakamura; L.F.C. Ribeiro; M.A. Fernandez
3/30/2017
Animals; Cattle; Female; Gene expression; In Vitro Techniques; Mammary Glands, Animal; Mastitis, Bovine; Milk; Models, Animal; Perfusion; Real-time polymerase chain reaction; Ubiquitin

Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six ... more

I.S.B. Pinto; I. Fonseca; H.M. Brandão; J.C. Gern; A.S. Guimarães; W.A. Carvalho; M.A.V.P. Brito; L.F. Viccini; M.F. Martins
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Disease resistance; Evolution; Expressed sequence tag-simple sequence repeat; Genetic diversity; Gossypium

Cotton (Gossypium spp) is one of the most economically important crops that provide the world’s most widely used natural fiber. Diseases such as Fusarium wilt and particularly Verticillium wilt seriously affect cotton production, and thus breeding for disease resistance is one of the most important goals of cotton breeding programs. Currently, potential exists to improve disease ... more

B.H. Wang; P. Rong; X.X. Cai; W. Wang; X.Y. Zhu; C.J. Chen; Y.Y. Xu; X.J. Huang; Z.M. Zhuang; C.B. Wang
08/14/2015
Carotenoids; Evolution; Phytoene synthase

In this study, we examined phytoene synthetase (PSY), the first key limiting enzyme in the synthesis of carotenoids and catalyzing the formation of geranylgeranyl pyrophosphate in terpenoid biosynthesis. We used known amino acid sequences of the PSY gene in tomato plants to conduct a genome-wide search and identify putative candidates in 34 sequenced plants. A total of 101 homologous ... more

Y. Han; Q.S. Zheng; Y.P. Wei; J. Chen; R. Liu; H.J. Wan
07/14/2015
Database; EST; Evolution; Nicotiana; Primer; Single nucleotide polymorphism

Single nucleotide polymorphisms (SNPs) are widespread in the Nicotiana genome. Using an alignment and variation detection method, we developed 20,607,973 SNPs, based on the expressed sequence tag sequences of 10 Nicotiana species. The replacement rate was much higher than the transversion rate in the SNPs, and SNPs widely exist in the Nicotiana. In vitro ... more

Y. Wang; D. Zhou; S. Wang; L. Yang
10/27/2015
Abiotic stress; bZIP; Carrot; Evolution; Group A; Transcription factor

The basic-region/leucine-zipper (bZIP) family is one of the major transcription factor (TF) families associated with responses to abiotic stresses. Many members of group A in this family have been extensively examined and are reported to perform significant functions in ABA signaling as well as in responses to abiotic stresses. In this study, 10 bZIP factors in carrot were classified ... more

F. Que; G.L. Wang; Y. Huang; Z.S. Xu; F. Wang; A.S. Xiong

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