Research Article

Related GMR Articles

Endemic species; Genetic diversity; Pinus krempfii; Population structure; SSR markers

Pinus krempfii Lecomte (Pinaceae) is an endemic tree to Vietnam with restricted habitats at higher altitudes in the highlands. In this study, genetic variation of four populations of P. krempfii was assessed using 17 microsatellite markers (single sequence repeats). Of these 17 markers, eight were polymorphic, and among the 42 putative alleles amplified, 32 were polymorphic ( ... more

D.T. Phong; T.T. Lieu; V.T.T. Hien; N.T. Hiep
Coilia nasus; De novo transcriptome; Illumina sequencing; SSR markers

RNA-Seq technology has been widely applied to tran­scriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence re­peats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enrichment, only a few microsatellite markers ... more

D.A. Fang; Y.F. Zhou; J.R. Duan; M.Y. Zhang; D.P. Xu; K. Liu; P. Xu; Q. Wei
Ammopiptanthus mongolicus; De novo transcriptome; eggNOG analysis; GO functional analysis; KEGG pathway analysis; SSR markers

Ammopiptanthus mongolicus, an evergreen broadleaf legume shrub, can survive under conditions of high and low temperature, extreme salinity, and drought. This attribute makes it an ideal model for studying mechanisms of stress tolerance in plants. However, simple sequence repeat (SSR) resources for this species are insufficient in public databases. In this study, a total of 44,959 ... more

M. Jin; M.Y. Guo; L. Han; J.L. Li; S.Y. Yang; Y.H. Su; M. Jin; M.Y. Guo; L. Han; J.L. Li; S.Y. Yang; Y.H. Su
Genetic variability; Manihot esculenta; SSR markers

Cassava is one of the main energy foods for millions of people, and has a great diversity of ethno-varieties that have specific characteristics often not found in commercial varieties. These constitute a gene pool and therefore a genetic resource that should be conserved and preserved. In this context, the objective of our study was to evaluate the genetic ... more

A.V. Tiago; E.S.S. Hoogerheide; E.C.M. Pedri; F.S. Rossi; E.S. Cardoso; J.M.A. Pinto; G.F. Pena; A.A.B. Rossi
Conservation; Genetic diversity; Simple sequence repeat; Wild soybean

Annual wild soybean (Glycine soja Sieb. et Zucc.), the ancestor of cultivated soybean (G. max), is believed to be a potential gene source for further improvement of soybean to cope with environmental stress. In this study, 10 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population genetic structure in five wild soybean populations using ... more

Y.H. Wang; X.J. Zhang; S.J. Fan
Genetic diversity; Genic-SSRs; Nelumbo nucifera; Transcriptome

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across ... more

X.F. Zheng; Y.N. You; Y. Diao; X.W. Zheng; K.Q. Xie; M.Q. Zhou; Z.L. Hu; Y.W. Wang
Abelmoschus esculentus; Genetic diversity; Population structure; Retrotransposons; Turkish landraces

Lack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding ... more

M. Yildiz; M. Koçak; F.S. Baloch
Disease resistance; Evolution; Expressed sequence tag-simple sequence repeat; Genetic diversity; Gossypium

Cotton (Gossypium spp) is one of the most economically important crops that provide the world’s most widely used natural fiber. Diseases such as Fusarium wilt and particularly Verticillium wilt seriously affect cotton production, and thus breeding for disease resistance is one of the most important goals of cotton breeding programs. Currently, potential exists to improve disease ... more

B.H. Wang; P. Rong; X.X. Cai; W. Wang; X.Y. Zhu; C.J. Chen; Y.Y. Xu; X.J. Huang; Z.M. Zhuang; C.B. Wang
Curcuma wenyujin; Genetic diversity; Inter simple sequence repeat (ISSR); Phylogenetic analysis; Population; Random amplified polymorphic DNA (RAPD)

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of ... more

W.H. Zheng; Y. Zhuo; L. Liang; W.Y. Ding; L.Y. Liang; X.F. Wang
Genetic diversity; Random amplification of polymorphic DNA; Zea mays

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To ... more

F.S. Resh; C.A. Scapim; C.A. Mangolin; M.F.P.S. Machado; A.Tdo Amaral; H.C.C. Ramos; M. Vivas