Research Article

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HER2/neu; Prostate cancer; RNA interference; Small-interfering RNA

The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposomes, a negative siRNA sequence, or ... more

C.Y. Liu; P.C. Xu; D.G. Chen; X.H. Fan; M.Q. Li; X. Yang; Y.P. Xu
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CapG; Cell growth; Invasion; Metastasis; Prostate cancer; RNA interference

This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), ... more

B.K. Li; K. Guo; C.Y. Li; H.L. Li; P.P. Zhao; K. Chen; C.X. Liu
11/19/2015
Down-regulation effect; Hepatocellular carcinoma; Osteopontin; RNA interference

Osteopontin (OPN) plays an important role in the metastasis and recurrence of tumors after resection of hepatocellular carcinoma (HCC). In this study, the down-regulation effect on OPN expression in HCC cells of RNA interference (RNAi) molecules designed to target different segments of OPN was investigated to identify a more effective site for OPN knockdown. Specific small interfering ... more

F. Lin; C.M. Huang; J. Cao; Z.H. Pei; W.L. Gu; S.F. Fan; K.P. Li; C.M. Lin
04/04/2016
Livin; Nanoparticle; PC-3M; Prostate cancer; RNA interference; Survivin

Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by ionic crosslinking. Through electrostatic adsorption, mPEG-CS- ... more

A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman
01/23/2017
Apoptosis; Cell Line, Tumor; Cell proliferation; Female; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; Proto-Oncogene Proteins c-bcl-2; RNA interference; Uterine cervical neoplasms

Cervical carcinoma is a life-threatening illness posing considerable danger to women's health. microRNAs (miRNAs) have been shown to regulate multiple cellular events, including growth and proliferation, and miR-187 is thought to regulate the growth and apoptosis of certain cell types. Our study focused on the influence of miR-187 on the growth, proliferation, and apoptosis of SiHa cervical ... more

C.Y. He; J. Yang
10/05/2017
Bmice-2; ilkworm; RNA interference; RNAI vector
Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA. The backbone of interference vector ... more
H. Yi; X. Ma
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Acute coronary syndromes; Cardiovascular disease; Coronary artery disease; Estrogen receptor 1; Meta-analysis; Myocardial infarction

The aim of this meta-analysis was to evaluate the correlations between the estrogen receptor 1 (ESR1) gene polymorphisms PvuII (rs2234693T>C) and XbaI (rs9340799A>G) and the risk of cardiovascular disease (CVD). Case-control studies were screened and selected from a larger group of studies that were retrieved through a comprehensive search of scientific ... more

N. Jiang; G. Yang; C.L. Peng
10/05/2015
Interleukin factors; Myocardial infarction; Polymorphism

Myocardial infarction (MI) is currently a leading cause of death worldwide, and is caused by various environmental and genetic factors. We therefore conducted a case-control study to investigate the association between polymorphisms in interleukins IL-1β, IL-8, and IL-10 and MI risk. This study recruited 260 MI patients and 285 control subjects. Genotyping of IL-1β +3954C/T, IL-8 -251T/A, ... more

S. Wang; Y.X. Dai; L.L. Chen; T. Jiang; M.Q. Zheng; C.G. Li; Y.P. Chen; W.H. Lin; J.F. Zhang; J. Jiang
03/04/2016
Atherothrombosis; Matrix metalloproteinase; Myocardial infarction; Polymorphisms

Several studies have demonstrated that matrix metalloproteinases (MMPs) play a major role in atherosclerotic plaque disruption and lead to myocardial infarction (MI). We investigated the association between the MMP1 -1607 1G/2G (rs1799750), MMP3 -1612 5A/6A (rs3025058), and MMP9 -1562 C/T (rs3918242) polymorphisms and the risk of developing MI in a ... more

J.M. Rodríguez-Pérez; G. Vargas-Alarcón; R. Posadas-Sánchez; T.X. Zagal-Jiménez; R. Ortíz-Alarcón; B. Valente-Acosta; C. Tovilla-Zárate; C. Nostroza-Hernández; O. Pérez-Méndez; N. Pérez-Hernández; J.M. Rodríguez-Pérez; G. Vargas-Alarcón; R. Posadas-Sánchez; T.X. Zagal-Jiménez; R. Ortíz-Alarcón; B. Valente-Acosta; C. Tovilla-Zárate; C. Nostroza-Hernández; O. Pérez-Méndez; N. Pérez-Hernández