Research Article

Related GMR Articles

Callus; Gene cloning; Isoflavone synthase; Sequence analysis; Trifolium pretense

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and ... more

H.H. Hu; C.Q. Jing; R. Liu; W.D. Li; H.G. Feng
Biological evolution; Gossypium raimondii; Phylogeny; Sequence analysis; Synteny; TIFY gene family

The highly conserved TIFY domain is included in the TIFY protein family of transcription factors, which is important in plant development. Here, 28 TIFY family genes were identified in the Gossypium raimondii genome and classified into JAZ (15 genes), ZML (8), PPD (3), and TIFY (2). The normal (TIF[F/Y]XG) motif was dominant in the TIFY ... more

D.H. He; Z.P. Lei; B.S. Tang; H.Y. Xing; J.X. Zhao; Y.L. Jing
Aleurites moluccana L.; BCCP subunit gene; Expression analysis; Molecular cloning; Sequence analysis

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. ... more

W.Y. Xuan; Y. Zhang; Z.Q. Liu; D. Feng; M.Y. Luo
Genotype; Hepatitis B virus; Sequence analysis

Hepatitis B virus genotype C (HBV/C) has the largest number of subgenotypes (C1-C16) that vary with geography and isolates. HBV/C prevails in Southeast Asia (C1, C5-C16), East Asia (C2), Oceania (C3), and Australia (C4). Suitable reference strains for different subgenotypes could greatly facilitate research into HBV/C, but unfortunately they are scarce. We retrieved 974 HBV/C full-length ... more

H.L. Zhu; C.T. Wang; J.B. Xia; X. Li; Z.H. Zhang
Alligator sinensis; cDNA cloning; Internal reference; Sequence analysis; β-actin

β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that ... more

H.N. Zhu; S.Z. Zhang; Y.K. Zhou; C.L. Wang; X.B. Wu
Abiotic stress; Medicago sativa L.; Peroxidase; Transgenic Arabidopsis thaliana

Peroxidases (PODs) are enzymes that play important roles in catalyzing the reduction of H2O2 and the oxidation of various substrates. They function in many different and important biological processes, such as defense mechanisms, immune responses, and pathogeny. The POD genes have been cloned and identified in many plants, but their function in alfalfa (Medicago ... more

K. Teng; G.Z. Xiao; W.E. Guo; J.B. Yuan; J. Li; Y.H. Chao; L.B. Han; K. Teng; G.Z. Xiao; W.E. Guo; J.B. Yuan; J. Li; Y.H. Chao; L.B. Han
Gene cloning; Hami melon; Real-time fluorescence quantitative PCR; Superoxide dismutase

Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was ... more

C.H. Shan; F.X. Tang; W. Chen; W.R. Ma
Eruca vesicaria subsp sativa; Gene cloning; Nicotianamine synthase

Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a ... more

B.L. Huang; C. Cheng; G.Y. Zhang; J.J. Su; Y. Zhi; S.S. Xu; D.T. Cai; X.K. Zhang; B.Q. Huang
Barley; Drought stress; Gene cloning; Gene expression; HbSINA4; Hordeum vulgare

Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety ‘Himalaya 10’ and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of HbSINA4 was 1052 bp long, including an open reading frame of 771 ... more

H.J. Yuan; X.M. Luo; T.S. Nyima; Y.L. Wang; Q.J. Xu; X.Q. Zeng
Agropyron mongolicum Keng; Cysteine protease gene; Expression analysis; Gene cloning

In this study, a cysteine protease gene (MwCP) from Agropyron mongolicum Keng was isolated using RACE. Sequence analysis indicated that MwCP was 1473 bp, and it contained a 1134-bp open reading frame, which encoded 377 amino acids with a 24-amino acid N-terminal signal peptide. The results indicated that the MwCP protein was a new member of the papain C1A family ... more

T.G.B.Y. Ao; M.L. Lang; Y.Q. Li; Y. Zhao; L.C. Wang; X.J. Yang; T.G.B.Y. Ao; M.L. Lang; Y.Q. Li; Y. Zhao; L.C. Wang; X.J. Yang; T.G.B.Y. Ao; M.L. Lang; Y.Q. Li; Y. Zhao; L.C. Wang; X.J. Yang