Research Article

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MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the plant kingdom, using the miRBase database. Some conserved miRNA ... more

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To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta ( ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; Z. Xinbo
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marker-assisted selection; Psathyrostachys huashanica; RAPD; Repetitive sequence; SCAR; Wheat

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that ... more

W.L. Du; J. Wang; L.M. Wang; Y.H. Pang; J. Wu; J.X. Zhao; Q.H. Yang; X.H. Chen
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MicroRNA; Northern blot; UV-B radiation; Wheat

MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the ... more

B. Wang; Y.F. Sun; N. Song; X.J. Wang; H. Feng; L.L. Huang; Z.S. Kang
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A spontaneous dwarf mutant of wheat was found in an F5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and ... more

X.J. Jin; D.F. Sun; H.Y. Li; Y. Yang; G.L. Sun
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Aflatoxin; Aspergillus flavus; HPLC; Inter-simple sequence repeats; Random amplified polymorphic DNA; Wheat

Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus ... more

A.S. Al-Wadai; M.R. Al-Othman; M.A. Mahmoud; A.R.M.Abd El-Aziz
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Chromosome behavior; Genomic in situ hybridization; Meiosis; mitosis; Rye; Wheat

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Consensus map; Humid; QTL; Quality; Rain-fed agriculture; Wheat

Humid rain-fed agriculture is a special environment for wheat (Triticum aestivum) culture that tends to negatively affect wheat yield and quality. To identify quality characters of wheat in a humid environment, we conducted quality analysis and quantitative trait loci (QTL) detection in a recombinant inbred line whose parent had a high level of quality for several years. We found ... more

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Bulked segregant analysis; Drought tolerance; QTL; SSR markers; Wheat

This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress ... more

M.R.Naroui Rad; A. Kadir; M.Y. Rafii; H.Z.E. Jaafar; M.R. Naghavi
08/16/2012
Amino acid indexes; Grain softness protein; Rye; Triticale; Wheat

Grain softness protein (GSP) is an important protein for overcoming milling and grain defenses in the innate immunity systems of cereals. The objective of this study was to evaluate and understand GSP sequences in selected wheat, rye and triticale. Using sequences for this gene from a sequence database, we performed clustering analysis to compare the sequences obtained from 3 germplasms ... more

M.A.S. Kharrazi; V. Bobojonov

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