Research Article

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01/29/2016
Anthocyanin; Red leaf lettuce; Simple sequence repeat; Transcriptome

This study aimed to analyze the transcriptome profile of red lettuce and identify the genes involved in anthocyanin accumulation. Red leaf lettuce is a popular vegetable and popular due to its high anthocyanin content. However, there is limited information available about the genes involved in anthocyanin biosynthesis in this species. In this study, transcriptomes of 15-day-old seedlings ... more

Y.Z. Zhang; S.Z. Xu; Y.W. Cheng; H.Y. Ya; J.M. Han; Y.Z. Zhang; S.Z. Xu; Y.W. Cheng; H.Y. Ya; J.M. Han; Y.Z. Zhang; S.Z. Xu; Y.W. Cheng; H.Y. Ya; J.M. Han
04/25/2016
Gene Ontology; Grass carp hemorrhage; Grass carp reovirus; High-throughput sequencing; KEGG; Transcriptome sequencing

The aim of the study was to investigate the grass carp hemorrhagic infection pathway and its key-related genes. Grass carp reovirus (GCRV) might cause hemorrhagic disease in grass carps. Healthy grass carp fingerlings (N = 60) were divided into control and infected groups. Fish in the control group were intraperitoneally (ip) injected with 0.6% fish physiological saline; the ... more

B.H. Xu; L. Zhong; Q.L. Liu; T.Y. Xiao; J.M. Su; K.J. Chen; H.Q. Wang; Y.J. Dai; J. Chen; B.H. Xu; L. Zhong; Q.L. Liu; T.Y. Xiao; J.M. Su; K.J. Chen; H.Q. Wang; Y.J. Dai; J. Chen; B.H. Xu; L. Zhong; Q.L. Liu; T.Y. Xiao; J.M. Su; K.J. Chen; H.Q. Wang; Y.J. Dai; J. Chen
03/30/2022
Amplicon sequencing; AmpliSeq; Coronavirus; COVID-19; High-throughput sequencing; Viral genome

The SARS-CoV-2 pandemic has demonstrated the need for genomic epidemiology surveillance. To date, various methodologies have been applied, including metagenomic approaches and amplicon-based sequencing associated with high-throughput sequencing platforms. We adapted some details in amplicon-based sequencing using a SARS-CoV-2 community panel (Illumina AmpliSeq), ... more

C.P. Targueta; R.S. Braga-Ferreira; A.A. de Melo; J.S. de Curcio; R. Nunes; R.O. Dias; F. Mello-Andrade; D.M. Silva; E.P. Silveira-Lacerda; T.G. Castro; T.M.A. Pedroso; L.A. Pereira; A.F. Mendonça; R.M. Almeida; V.L. Silva; M.P.C. Telles
09/22/2015
Genetic diversity; Genic-SSRs; Nelumbo nucifera; Transcriptome

Nelumbo nucifera is an important economic vegetable and traditional medicine, but available genetic resources remain limited. Next generation sequencing has proven to be a rapid and effective means of identifying genic simple sequence repeat (genic-SSR) markers. This study developed genic-SSRs for N. nucifera using Illumina sequencing technology to assess diversity across ... more

X.F. Zheng; Y.N. You; Y. Diao; X.W. Zheng; K.Q. Xie; M.Q. Zhou; Z.L. Hu; Y.W. Wang
07/28/2015
Heat shock protein; high temperature; Transcriptome; Tree peony leaf

The tree peony leaf is an important vegetative organ that is sensitive to abiotic stress and particularly to high temperature. This sensitivity affects plant growth and restricts tree peony distribution. However, the transcriptomic information currently available on the peony leaf in public databases is limited. In this study, we sequenced the transcriptomes of peony leaves subjected to ... more

Y.Z. Zhang; Y.W. Cheng; H.Y. Ya; J.M. Han; L. Zheng
07/28/2015
Assembly; Brontispa longissima; Transcriptome

The coconut leaf beetle, Brontispa longissima, is a de­structive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular ba­sis of biology and ecology, we sequenced and analyzed its whole tran­scriptome by using high-throughput Illumina paired- ... more

W. Yan; L. Liu; C.X. Li; S.C. Huang; Z.L. Ma; W.Q. Qin; Z.Q. Peng
07/06/2015
Chemoreception; Expression pattern; Rhynchophorus ferrugineus; Transcriptome

Olfaction is crucial for insects’ survival because it enables them to recognize various environmental information. It is primarily mediated by a large family of chemoreceptors, including olfactory receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs). Here, we assembled the transcriptome of the economically important pest of palms, Rhynchophorus ferrugineus, to ... more

W. Yan; L. Liu; W.Q. Qin; C.X. Li; Z.Q. Peng
12/29/2015
Acipenser schrenckii; Ovaries; Reproduction; Testes; Transcriptome

Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A total of 122,381 ... more

S.B. Jin; Y. Zhang; X.L. Dong; Q.K. Xi; D. Song; H.T. Fu; D.J. Sun
12/29/2015
Cocoa tea; Theobromine biosynthesis; Trans-catechins biosynthesis; Transcriptome

Camellia ptilophylla, or cocoa tea, is naturally decaffeinated and its predominant catechins and purine alkaloids are trans-catechins and theobromine Regular tea [Camellia sinensis (L.) O. Ktze.] is evolutionarily close to cocoa tea and produces cis-catechins and caffeine. Here, the transcriptome of C. ptilophylla was sequenced using the 101- ... more

M.M. Li; J.Y. Xue; Y.L. Wen; H.S. Guo; X.Q. Sun; Y.M. Zhang; Y.Y. Hang
12/22/2015
Alkalinity stress; Carbonate; Nile tilapia; Transcriptome

To identify genes of potential importance to alkalinity tolerance, RNA sequencing (RNA-Seq) was performed to survey gill transcriptome profiles from freshwater (FW) and alkaline water (AW) exposed Nile tilapia (Oreochromis niloticus). A total of 22,724,036 (AW)/16,461,040 (FW) single-end reads were generated in which 20,304,348 (AW)/14,681,290 (FW) reads (90.0/89.72%) were ... more

Y. Zhao; J. Wang; J. Thammaratsuntorn; J.W. Wu; J.H. Wei; Y. Wang; J.W. Xu; J.L. Zhao

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