Research Article

Related GMR Articles

CDC16; Chromosomal localization; Expression profile; Immunohistochemistry; Pig

Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amplification of cDNA ends (RACE). Porcine ... more

D. Xu; J. Jiang; H. Ma
Cloning; Expression profiles; Pig; Single nucleotide polymorphism; SLC13A5

Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of cDNA ends technique. Full-length porcine ... more

L.Y. Wang; J. Jiang; H.M. Ma
Follicle-stimulating hormone receptor; Number born alive; Pig; Polymorphism; Total number born

Follicle-stimulating hormone (FSH), a glycoprotein secreted by the anterior pituitary, can regulate ovarian function through the FSH receptor (FSHR). To evaluate the effects of the FSHR gene on reproductive traits in pigs, polymorphisms in exon 10 of the FSHR gene were observed by polymerase chain reaction-single-strand conformation polymorphism, and 3 single ... more

X.D. Zhang; H.Y. Zhu; J. Zhou; N. Wang; N. Zhou; L. Huang; T. Wu; Y.F. Feng; Y.Y. Ding; Z.J. Yin
Global cerebral ischemia-reperfusion; Immunohistochemistry; Intermittent hypobaric hypoxia

This study was aimed at understanding the effect of intermittent hypobaric hypoxia preconditioning (IHHP) on neuroglobin (NGB) and Bcl-2 expression in the hippocampal CA1 region of rats following global cerebral ischemia-reperfusion. Wistar rats were randomly divided into sham, IHHP control, global cerebral ischemia-reperfusion (IR group), and IHHP+IR groups. The four-vessel occlusion rat ... more

Q. Wu; K.X. Yu; Q.S. Ma; Y.N. Liu
Colorectal cancer; Epithelial cell adhesion molecule; Immunohistochemistry

We investigated the relationship between the expression of epithelial cell adhesion molecule (EpCAM) and the occurrence and development of colon cancer. Fifty colon cancer tissues and adjacent normal tissues were collected, while 40 normal intestinal mucosa tissues were collected as the blank group. EpCAM expression was detected by immunohistochemistry and the patients were followed-up to ... more

X.B. Chai; R.F. Song; F. Xu
ELISA; Immunohistochemistry; Polyclonal antibody; Porcine β-defensin 2; Western blotting

Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti ... more

Y.Y. Bao; L. Li; H. Zhang; C.Y. Gao; C.B. Xiao; C.L. Li
Immunohistochemistry; Magnetic resonance perfusion weighted imaging; Radiation-induced brain injury; Vascular endothelial growth factor

We used magnetic resonance perfusion weighted imaging and pathological evaluation to examine different stages of radiation-induced brain injury and to investigate the correlation between the relative cerebral blood volume (rCBV) ratio and vascular endothelial growth factor (VEGF). Thirty adult rats were randomly divided into 2 groups: control and radiation group. The control group was not ... more

X.J. Liu; C.F. Duan; W.W. Fu; L. Niu; Y. Li; Q.L. Sui; W.J. Xu
c-Myc; Carcinoma papillary; Immunohistochemistry; Mucin 1; Proto-oncogene proteins; Thyroid

The aim of this study was to examine the expression of mucin 1 (MUC1) and c-myc and the significance thereof in elderly patients with papillary thyroid carcinoma. The expression levels of MUC1 and c-myc were examined by immunohistochemical methods in 58 patients with papillary thyroid carcinoma, 35 with nodular goiter, and 30 subjects with normal thyroid tissue. The positive rate of MUC1 ... more

Y.J. Hu; X.Y. Luo; Y. Yang; C.Y. Chen; Z.Y. Zhang; X. Guo
Immunohistochemistry; Intervertebral disc nucleus pulposus; MMP-1; Resveratrol; SIRT1

Intervertebral disc degeneration is the main cause of lumbago disease, in which the extracellular matrix structure and moisture in the nucleus pulposus is lost continuously. In this study, we aimed to detect differential expression of silence mating type information regulation 2 homolog 1 (SIRT1) and matrix metalloproteinase-1 (MMP-1) in human intervertebral disc nucleus pulposus cells ... more

J.W. Wu; J.J. Wang; J.B. Chen; Y.L. Huang; H. Wang; G.H. Liu; L.F. Li; M. Kang; X.G. Wang; H.H. Cai
Cervical cancer; Ebp1; Immunohistochemistry; p53

In this study, the ErbB3-binding protein (Ebp1) and p53 protein expression in cervical cancer tissues, and its significance in the prognosis of the disease was investigated. Ebp1 and p53 protein expression was detected by immunohistochemical analysis in cervical cancer tissues (N = 60) and normal tissues adjacent to the cancer tissues (N = 60). The rates of positive Ebp1 and p53 protein ... more

L. Liu; X.D. Li; H.Y. Chen; J.S. Cui; D.Y. Xu