Research Article

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Prophenoloxidase activating factors (PPAFs) are a group of clip domain serine proteinases that can convert prophenoloxidase (pro-PO) to the active form of phenoloxidase (PO), causing melanization of pathogens. Here, two full-length PPAF cDNAs from Scylla paramamosain (SpPPAF1 and SpPPAF2) were cloned and characterized. The full-length SpPPAF1 cDNA was ... more

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In this study, we analyzed the correlation of 20 growth-related traits and their effects on body weight of Scylla paramamosain. The correlation coefficients in all trait pairs were significantly high, ranging from 0.551 to 0.999. Among 19 X-Y pairs, the correlation coefficient between traits X1 and Y was the highest, whereas that ... more

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Scylla paramamosain is a widespread and commercially important species of coastal marine crab. We identified 13 polymorphic microsatellite loci from a genome library constructed with 5ꞌ-anchored PCR method. Thirty-two S. paramamosain from the East China Sea were used to analyze the characteristics of these loci. The number of alleles per locus ranged from 3 to 8, with a ... more

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Quantitative real-time polymerase chain reaction; Scylla paramamosain; Thioredoxin reductase; Thioredoxin reductase activity; Thioredoxin-1 gene; Tissue expression

The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity ... more

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Correlation analysis; Growth traits; Multiple regression equation; Path analysis; Scylla paramamosain

The mud crab (Scylla paramamosain) is considered a potentially important marine crab species for selective breeding. Here, we first examined sex ratio and differences in 16 growth traits between females and males in a G1 population of S. paramamosain, and we then analyzed the correlation between these growth traits and their effects on body weight (BW). Of ... more

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Endogenous control gene; Grain filling; Quantitative real-time PCR; Triticum aestivum L.

The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, NormFinder, and BestKeeper, were ... more

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Evolutionarily conserved signaling intermediate in Toll pathways (Ecsit) is reported to play an essential role in innate immunity, embryogenesis, and assembly or stability of the mitochondrial complex I. In this study, the full-length cDNA of Ecsit was cloned from the spinyhead croaker Collichthys lucidus based on the expressed sequence tags from our cDNA library constructed ... more

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