Research Article

Related GMR Articles

Genetic diversity; Pistacia vera; SRAP markers; Structure analysis

Sequence-related amplified polymorphism (SRAP) markers preferentially amplify open reading frames and were used to study the genetic diversity of Tunisian pistachio. In the present study, 43 Pistacia vera accessions were screened using seven SRAP primer pairs. A total of 78 markers was revealed (95.12%) with an average ... more

K. Guenni; M. Aouadi; K. Chatti; A. Salhi-Hannachi; K. Guenni; M. Aouadi; K. Chatti; A. Salhi-Hannachi
Cis-acting element; Dof transcription factor; Expression patterns; Genomic duplication; Medicago truncatula; Phylogenetic analysis

The DNA-binding one zinc finger (Dof) family is a classic plant-specific zinc-finger transcription factor family, which is involved in many important processes, including seed maturation and germination, plant growth and development, and light responses. Investigation of the Medicago truncatula genome revealed 42 putative Dof genes, each of which holds one Dof domain. These genes ... more

Y.J. Shu; L.L. Song; J. Zhang; Y. Liu; C.H. Guo
Curcuma wenyujin; Genetic diversity; Inter simple sequence repeat (ISSR); Phylogenetic analysis; Population; Random amplified polymorphic DNA (RAPD)

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of ... more

W.H. Zheng; Y. Zhuo; L. Liang; W.Y. Ding; L.Y. Liang; X.F. Wang
16S rRNA gene; Agronomic crops; Bioprospection; Biotechnology; Endophytic bacteria; Phylogenetic analysis

Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size, and availability of databases ... more

C. Bredow; J.L. Azevedo; J.A. Pamphile; C.A. Mangolin; S.A. Rhoden
Phylogenetic analysis; Rhoptry protein 20; Sequence variation; Toxoplasma gondii; Toxoplasmosis

Toxoplasma gondii, an opportunistic protozoan parasite, infects almost all warm-blooded animals. In this study, we examined the sequence variation in rhoptry protein 20 (ROP20) genes among 18 T. gondii isolates collected from different hosts and geographical regions. Full length ROP20 genes were amplified and sequenced. The results showed that the genes were ... more

H.R. Ning; J. .L.Wang; S.Y. Qin; S.Y. Huang; Z.L. Lou; L.Y. Hu; X.Q. Zhu
Banna mini-pig inbred line; Expression pattern; Gene structure; Phylogenetic analysis; Splice isoform; Testis-specific serine kinase

Testis-specific serine kinases (TSSKs) are a family of serine/threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, ... more

P. Wang; H.L. Huo; S.Y. Wang; Y.W. Miao; Y.Y. Zhang; Q.L. Zhang; F.Q. Li; L.X. Liu; W.Z. Li; Y.Z. Zeng; J.L. Huo; H. Xiao
Gene expression pattern analysis; Gene structure; GRAS gene family; Phylogenetic analysis; Protein motif analysis; Tobacco

Members of the GRAS gene family are important transcriptional regulators. In this study, 21 GRAS genes were identified from tobacco, and were classified into eight subgroups according to the classification of Arabidopsis thaliana. Here, we provide a preliminary overview of this gene family in tobacco, describing the gene structure, gene expression, protein motif ... more

Y.Q. Chen; S.S. Tai; D.W. Wang; A.M. Ding; T.T. Sun; W.F. Wang; Y.H. Sun
Chloroplast genome; Conserved sequence; Phylogenetic analysis; species identification; Universal primer; Variable sequence

Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. To identify conserved regions that can be used to design primers and amplify variable sequences for use in plant phylogenetic studies, the ... more

Y.C. Yang; T.L. Kung; C.Y. Hu; S.F. Lin
Gene; Phylogenetic analysis; Porcine epidemic diarrhea; Virus

Porcine epidemic diarrhea poses significant sanitation problems in the porcine industry, and has negatively affected the economy in recent years. In this study, 48 fecal specimens were collected from piglets from four intensive swine farms located in the Gansu Province of China. The molecular diversity and phylogenetic relationships between porcine epidemic diarrhea viruses (PEDV) ... more

M.Z. Huang; H. Wang; S.Y. Wang; D.A. Cui; X. Tuo; Y.M. Liu; M.Z. Huang; H. Wang; S.Y. Wang; D.A. Cui; X. Tuo; Y.M. Liu; M.Z. Huang; H. Wang; S.Y. Wang; D.A. Cui; X. Tuo; Y.M. Liu
Endophytes; Enzymes; Fungi; Phylogenetic analysis

Plants of medicinal and economic importance have been studied to investigate the presence of enzyme-producing endophytic fungi. The characterization of isolates with distinct enzyme production potential may identify suitable alternatives for specialized industry. At Universidade Estadual de Maringá Laboratory of Microbial Biotechnology, approximately 500 isolates ... more

R.N. Alberto; A.T. Costa; J.C. Polonio; M.S. Santos; S.A. Rhoden; J.L. Azevedo; J.A. Pamphile; R.N. Alberto; A.T. Costa; J.C. Polonio; M.S. Santos; S.A. Rhoden; J.L. Azevedo; J.A. Pamphile