Research Article

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12/16/2015
HER2/neu; Prostate cancer; RNA interference; Small-interfering RNA

The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposomes, a negative siRNA sequence, or ... more

C.Y. Liu; P.C. Xu; D.G. Chen; X.H. Fan; M.Q. Li; X. Yang; Y.P. Xu
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Livin; Nanoparticle; PC-3M; Prostate cancer; RNA interference; Survivin

Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by ionic crosslinking. Through electrostatic adsorption, mPEG-CS- ... more

A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman; A.Q. Yang; P.J. Wang; T. Huang; W.L. Zhou; J. Landman
12/29/2015
A1298C; C677T; Meta-analysis; Methylenetetrahydrofolate reductase gene; Polymorphism; Prostate cancer

Genetic polymorphisms (C677T and A1298C) in methylenetetrahydrofolate reductase (MTHFR) were shown to be related to prostate cancer risk in previous studies; however, the results are controversial. We performed a meta-analysis of previous studies and quantitatively estimated these associations. Pubmed, Embase, and Cochrane Library Database were searched for published case-control studies ... more

P.L. Chen; W.T. Li; J. Wang; Y.D. Jiang; P. Wu; T. Chen; S.B. Zheng
10/28/2015
Gene expression; Nodular hyperplasia; PCA3; Prostate cancer; PSA

We evaluated the expression of the PCA3 gene in urine from patients with nodular hyperplasia/benign prostatic hyperplasia (PNH) or adenocarcinoma type prostate cancer (PCa).The study included 59 men: 22 with PCa, 26 with PNH, and 11 with no alterations (controls). Patients’ urine was collected following prostatic massage and quantified by quantitative real-time PCR for prostate ... more

F. Coelho; L. Guimarães; W.L.Ribeiro Cabral; P.G.Oliveira Salles; C. Mateo; M.Nogueira e Nogueira; C.E.Corradi Fonseca; B. Gomes
09/21/2015
Breast cancer; GIT2; Metastasis; MTERF; Multiplex RT-PCR

Quantitative multiplex reverse transcriptase-polymerase chain reaction was developed for the simultaneous detection of multiple-gene expression levels of formalin-fixed, paraffin-embedded breast cancer samples. Candidate genes were selected from previous microarray data relevant to breast cancer markers that had the potential to serve as predictive markers for metastatic risk. This multiplex ... more

S. Sirirattanakul; P. Wannakrairot; T. Tencomnao; R. Santiyanont
09/21/2015
EGFR; FISH; Metastasis; NSCLC

The aim of this study was to explore epidermal growth factor receptor (EGFR) gene amplification and its relationship with cancer invasion and metastasis in non-small cell lung cancer (NSCLC). EGFR amplification in 45 patients with NSCLC and 15 subjects with normal lung tissues was detected by fluorescence in situ hybridization. The relationship between EGFR ... more

X.F. Jia; J. Li; H.B. Zhao; J. Liu; J.J. Liu
12/16/2015
Bcl-2; Cervical cancer; KAI1; Metastasis; Survivin

Cervical cancer is associated with abnormal expression of multiple genes. Survivin and Bcl-2 proteins are apoptosis inhibitors. The tumor suppressor gene CD82, which encodes the protein KAI1, is downregulated in cervical cancer, and is associated with differentiation degree. We investigated the expression levels of three proteins and their correlation with metastasis in cervical ... more

X.L. Zhou; M. Wang
12/14/2015
Metastasis; MiR-134; Osteosarcoma; Prognosis; Proliferation

Dysregulation of microRNA (miR) is often associated with cancer development and progression. Aberrant expression of miR-134 has been found in some types of cancer. However, its expression and function in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-134 in osteosarcoma tumorigenesis and development. The expression level of miR-134 was quantified by ... more

Y. Bao; L. Peng; J. Ma; K. Liu; W. Li
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Metastasis; Neuroblastoma; Neuron-specific enolase; Nude mice; Xenograft

The aim of this study was to establish a metastatic human neuroblastoma (NB) mouse model by xenograft in order to study the metastatic mechanisms of NB. A human NB cell line was obtained from a 5-year-old patient and cultured in vitro. A suspension of these cells was subcutaneously inoculated into nude mice at the right flank next to the forelimb. The biological characteristics of the ... more

Q. Gao; C.F. Chen; Q. Dong; L. Hou; X. Chen; Y.L. Zhi; X. Li; H.T. Lu; H.Y. Zhang
12/08/2015
Chemical treatment; Dendritic cells; Immune treatment; Liver; Metastasis; Recurrence

We aimed to evaluate dendritic cell (DC) tumor vaccines for preventing liver cancer recurrence and metastasis. DCs were induced from mononuclear cells in the peripheral blood with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4), followed by sensitization with lysis of autologous liver cancer cells. One hundred and ... more

T.Y. Sun; W. Yan; C.M. Yang; L.F. Zhang; H.L. Tang; Y. Chen; H.X. Hu; X. Wei

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