Research Article

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04/04/2016
Biomarker; Breast cancer; Histological subtypes; Proteomics

Breast cancer is the second most common cancer worldwide and the first among women. Invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) are the two major histological subtypes, and the clinical and molecular differences between them justify the search for new markers to distinguish them. As proteomic analysis allows for a powerful and analytical approach to identify ... more

N.C.S. Oliveira; T.H.B. Gomig; H.H. Milioli; F. Cordeiro; G.G. Costa; C.A. Urban; R.S. Lima; I.J. Cavalli; E.M.S.F. Ribeiro; N.C.S. Oliveira; T.H.B. Gomig; H.H. Milioli; F. Cordeiro; G.G. Costa; C.A. Urban; R.S. Lima; I.J. Cavalli; E.M.S.F. Ribeiro; N.C.S. Oliveira; T.H.B. Gomig; H.H. Milioli; F. Cordeiro; G.G. Costa; C.A. Urban; R.S. Lima; I.J. Cavalli; E.M.S.F. Ribeiro
12/22/2015
Abiotic stress; Proteomics; RT-qPCR

The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome analysis. Seeds from six maize ... more

S.M.F. Dutra; E.V.R. Von Pinho; H.O. Santos; A.C. Lima; R.G. Von Pinho; M.L.M. Carvalho
12/02/2015
Drought stress; Larix olgensis families; Proteomics

The conifer Larix olgensis has been analyzed to delineate physiological and proteomic changes that occur under drought stress. Studies of the deleterious effects of drought in the larch families have mainly focused on photosynthesis. In the present study, when the intensity of drought was increased, plant height was inhibited as both POD and MDA levels increased, which indicates ... more

L. Zhang; H.G. Zhang; Q.Y. Pang
11/19/2015
Fermentation; Protein; Proteomics; Zymomonas mobilis

Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are ... more

D.R. Cavalcanti; C.B. Malafaia; T.D. Silva; B.S. Santos; G.M.T. Calazans; M.V. Silva
2/08/2017
Fruit; Meliaceae; Plant Extracts; Plant Proteins; Proteomics

We investigated the extraction of Toona sinensis fruit proteins and preliminarily characterized their physicochemical properties. The results showed that optimal extraction occurred under conditions of pH 10.5, a duration of 40 min, a liquid-to-solid ratio of 25:1, and a temperature of 40°C by an orthogonal design using T. sinensis fruit protein as the index and single factor. The total ... more

W.Z. Li; W.N. Han; B. Liu; S.H. Ding; X.K. Zhang; R.S. Wang
11/22/2013
Biomarker; Mutation; Myelodysplastic syndromes; Refractory cytopenias with multilineage dysplasia; TET2

Myelodysplastic syndrome diagnosis of karyotypically normal patients may be elusive because it relies exclusively on morphological and clinical data. In routine practice, finding of an acquired mutation or a cytogenetic abnormality provides irrefutable evidence of the clonal nature of that disease. Recurrent deletions and somatic mutations in TET2, a gene involved in epigenetic ... more

D.F. Coutinho; C. Diniz; R.L.D. Filgueiras; R.L.R. Baptista; J.P. Ayres-Silva; B.C.R. Monte-Mór; M.H. Bonamino; I.R. Zalcberg
09/28/2015
Biomarker; Diagnosis; HCC; miR-21; Prognosis

The diagnostic and prognostic value of miR-21 has been examined for hepatocellular carcinoma (HCC), with inconsistent results. Present meta-analysis summarized the diagnostic accuracy and the predictive role for survival of miR-21 in patients with HCC. All eligible studies were searched using PubMed, EMBASE, and Chinese National Knowledge Infrastructure (CNKI) databases up to October 2014 ... more

S.R. Yan; Z.J. Liu; S. Yu; Y.X. Bao
08/14/2015
Biomarker; Human kallikrein 5; Prognosis; Triple-negative breast cancer

The purposes of this study were to analyze the expression and distribution of human kallikrein 5 (hK5) in triple-negative breast cancer (TNBC) tissues, to establish a standard operating procedure (SOP) for its immunohistochemical assay, and to evaluate the possibility of hK5 being a prognostic biomarker for TNBC. Recombinant hK5 protein and specific antibody were prepared, and the expression ... more

F. Yang; J.Y. Li; Q.N. Yin; K. Yang; S.N. Dong; L.J. Bai; P. Liu; X.W. Tong
12/29/2015
Biomarker; Diagnosis; Ewing's sarcoma; miR-125; Serum

Previous studies indicated that microRNA-125b (miR-125b) has an important role in the progression of Ewing’s sarcoma (ES). The purpose of the current study was to examine expression changes of miR-125b in the serum of ES patients and evaluate if the expression level of miR-125b could serve as a new biomarker for ES. This study was performed on patients who underwent surgical resection at ... more

C.L. Nie; W.H. Ren; Y. Ma; J.S. Xi; B. Han
12/28/2015
Biomarker; Bladder cancer; Dickkopf-1; Prognosis

Dickkopf-1 (DKK-1) is a secreted protein that inhibits Wnt signaling. However, the clinical significance and prognostic value of serum DKK-1 levels have not been previously investigated in bladder cancer in Chinese patients. Blood samples were taken from 94 consecutive patients diagnosed with bladder cancer and 60 healthy control subjects. Serum DKK-1 expression levels were measured by ... more

D.K. Sun; L. Wang; J.M. Wang; P. Zhang

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