Research Article

Related GMR Articles

Cross-amplification; Genetic structure; Microsatellite loci; Taxus wallichiana var. wallichiana

Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of the 9 loci was observed in 2 other varieties ... more

B.B. Cheng; Q.W. Sun; Y.Q. Zheng
Microsatellite DNA; New species; Polymorphic loci; Sillago sinica

Sillago sinica is a newly identified species belonging to Sillaginidae, Perciforms, and was found along the coast of China in 2011. In the present study, 81 microsatellite loci were isolated from an enriched genomic library, and 24 positive clones containing microsatellite repeats had adequate flanking sequences for the development of PCR primers. Sixteen of these primers were ... more

N. Song; L.Y. Wang; L.N. Yin; Z.Q. Han; T.X. Gao; N. Song; L.Y. Wang; L.N. Yin; Z.Q. Han; T.X. Gao; N. Song; L.Y. Wang; L.N. Yin; Z.Q. Han; T.X. Gao
Biomarker; Mutation; Myelodysplastic syndromes; Refractory cytopenias with multilineage dysplasia; TET2

Myelodysplastic syndrome diagnosis of karyotypically normal patients may be elusive because it relies exclusively on morphological and clinical data. In routine practice, finding of an acquired mutation or a cytogenetic abnormality provides irrefutable evidence of the clonal nature of that disease. Recurrent deletions and somatic mutations in TET2, a gene involved in epigenetic ... more

D.F. Coutinho; C. Diniz; R.L.D. Filgueiras; R.L.R. Baptista; J.P. Ayres-Silva; B.C.R. Monte-Mór; M.H. Bonamino; I.R. Zalcberg
Biomarker; Diagnosis; HCC; miR-21; Prognosis

The diagnostic and prognostic value of miR-21 has been examined for hepatocellular carcinoma (HCC), with inconsistent results. Present meta-analysis summarized the diagnostic accuracy and the predictive role for survival of miR-21 in patients with HCC. All eligible studies were searched using PubMed, EMBASE, and Chinese National Knowledge Infrastructure (CNKI) databases up to October 2014 ... more

S.R. Yan; Z.J. Liu; S. Yu; Y.X. Bao
Biomarker; Human kallikrein 5; Prognosis; Triple-negative breast cancer

The purposes of this study were to analyze the expression and distribution of human kallikrein 5 (hK5) in triple-negative breast cancer (TNBC) tissues, to establish a standard operating procedure (SOP) for its immunohistochemical assay, and to evaluate the possibility of hK5 being a prognostic biomarker for TNBC. Recombinant hK5 protein and specific antibody were prepared, and the expression ... more

F. Yang; J.Y. Li; Q.N. Yin; K. Yang; S.N. Dong; L.J. Bai; P. Liu; X.W. Tong
Biomarker; Diagnosis; Ewing's sarcoma; miR-125; Serum

Previous studies indicated that microRNA-125b (miR-125b) has an important role in the progression of Ewing’s sarcoma (ES). The purpose of the current study was to examine expression changes of miR-125b in the serum of ES patients and evaluate if the expression level of miR-125b could serve as a new biomarker for ES. This study was performed on patients who underwent surgical resection at ... more

C.L. Nie; W.H. Ren; Y. Ma; J.S. Xi; B. Han
Biomarker; Bladder cancer; Dickkopf-1; Prognosis

Dickkopf-1 (DKK-1) is a secreted protein that inhibits Wnt signaling. However, the clinical significance and prognostic value of serum DKK-1 levels have not been previously investigated in bladder cancer in Chinese patients. Blood samples were taken from 94 consecutive patients diagnosed with bladder cancer and 60 healthy control subjects. Serum DKK-1 expression levels were measured by ... more

D.K. Sun; L. Wang; J.M. Wang; P. Zhang
Biomarker; Colorectal cancer; Diagnostic marker; Meta-analysis; MicroRNA-29a

Previous studies have revealed that the expression level of microRNA-29a (miR-29a) was remarkably different in colorectal cancer (CRC) patients and healthy controls, indicating that miR-29a can be used as a diagnostic marker of CRC, but the results have been inconsistent. We conducted this meta-analysis to assess the diagnostic performance of blood-based miR-29a for CRC. We performed a ... more

M.L. Zhi; Z.J. Liu; X.Y. Yi; L.J. Zhang; Y.X. Bao
Biomarker; Cervical cancer; Cervical intraepithelial neoplasia; miR-196a; Prognosis

Previous studies have reported that miR-196a is upregulated in cervical cancer tissues and cell lines. However, whether serum miR-196a is increased in patients with cervical cancer or cervical intraepithelial neoplasia (CIN), and its potential clinical value remained unknown. In total, 105 cervical cancer patients, 86 CIN patients, and 50 healthy volunteers were recruited. Quantitative ... more

P. Liu; F. Xin; C.F. Ma
Biomarker; Cutaneous melanoma; MicroRNA-106b; Prognosis

MicroRNA-106b (miR-106b) is overexpressed in various types of cancers and is associated with the regulation of carcinogenic processes. However, its clinical significance in cutaneous melanoma has not been reported. qRT-PCR was performed to examine the expression of miR-106b in 15 cases of dysplastic nevi, 17 cases of melanoma metastases, and 97 cases of primary cutaneous melanoma tissue ... more

N. Lin; Y. Zhou; X. Lian; Y. Tu