Research Article

Related GMR Articles

Acute myeloid leukemia cells; Cytokine-induced killer cells; Cytotoxic effect; DC-CIK; Dendritic cells

We cocultured cytokine-induced killer (CIK) cells with dendritic cells (DCs) in vitro and investigated their proliferation, immunophenotype changes, secretory cytokine levels, and their antitumor effects on acute myeloid leukemia (AML) cells. DCs and CIK cells were acquired from healthy human peripheral blood mononuclear cells and cocultured as an experimental group, while CIK ... more

X.Y. Cheng; J.L. Li
Chemokine; Dendritic cells; Graft-versus-host reaction; Immune tolerance; Recombinant lentivirus; Transgenic

We determined whether genetically engineered immature dendritic cells (imDCs) mediated by lentiviral vectors alleviate acute graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (allo-BMT) in mice. We introduced the mouse chemokine receptor 7 (Ccr7) gene into the bone marrow-derived imDCs of C57BL/6 mice to construct genetically engineered imDCs. A 1:1 ... more

Y.J. Xu; W.R. Chen; D.P. Li; L.X. Song; J.Q. Wu; P. Zhang; Z.Y. Li; Y.H. Huang
Concurrent chemoradiotherapy; Cytokine-induced killer cells; Dendritic cells; Non-small cell lung cancer

To compare the efficacy of dendritic and cytokine-induced killer cells (DC-CIK) therapy combined with concurrent radiochemotherapy on stage IIIB non-small cell lung cancer. Sixty-three patients with stage IIIB non-small cell lung cancer were randomly divided into the study and control groups. The study group, comprising 30 patients, was treated with DC-CIK combined with docetaxel- ... more

X.P. Zhu; Y.H. Xu; J. Zhou; X.F. Pan
Bladder tumor; Dendritic cell vaccine; Dendritic cells; MB49 cells

Herein, the preparation of a dendritic cell (DC) vaccine with radiation-induced apoptotic tumor cells and its immunological effects on bladder cancer in C57BL/6 mice was investigated. We used radiation to obtain a MB49 cell antigen that was sensitive to bone marrow-derived DCs to prepare a DC vaccine. An animal model of tumor-bearing mice was established with the MB49 mouse bladder cancer ... more

X.F. Xie; Q. Ding; J.G. Hou; G. Chen
Chemical treatment; Dendritic cells; Immune treatment; Liver; Metastasis; Recurrence

We aimed to evaluate dendritic cell (DC) tumor vaccines for preventing liver cancer recurrence and metastasis. DCs were induced from mononuclear cells in the peripheral blood with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4), followed by sensitization with lysis of autologous liver cancer cells. One hundred and ... more

T.Y. Sun; W. Yan; C.M. Yang; L.F. Zhang; H.L. Tang; Y. Chen; H.X. Hu; X. Wei
AE37; Cytokine-induced killer cells; Dendritic cells; HER2

This study aimed to investigate the cytotoxicity of cytokine-induced killer cells (CIKs) and Her2 epitope peptide-sensitized dendritic cells (DCs), when co-cultured with Her2-positive MCF-7 cells. DCs were separated from the Her epitope peptide-sensitized peripheral blood; the Her epitope combines directly with the MHC-II molecule on the DC surface. The DCs were co-cultured with ... more

Y.Y. Wen; X.S. Hu; Y.Y. Wen; X.S. Hu; Y.Y. Wen; X.S. Hu
Bone neoplasms; Osteochondroma; Osteosarcoma

Osteochondroma is a cartilage capped benign tumor developing mainly at the juxta-epiphyseal region of long bones. The rate of malignant transformation, mainly into chondrosarcoma, is estimated to be less than 1-3%. Transformation into osteosarcoma is very rare and has been reported only thirteen times. There is little information on treatment and outcome. We report the case of a secondary ... more

E.E. Engel; M.H. Nogueira-Barbosa; M.S. Brassesco; G.E.B. Silva; E.T. Valera; F.M. Peria; T.C. Motta; L.G. Tone
DNA repair genes; Osteosarcoma; Overall survival; Polymorphism

We conducted a perspective study to investigate the role of ERCC1 (rs11615), ERCC2 (rs13181 and rs1799793), ERCC4 (rs1800067), and ERCC5 (rs17655) in NER pathway in the prognosis of osteosarcoma patients. In total, 146 osteosarcoma patients were recruited between 2008 and 2013. ERCC1 rs11615, ERCC2 rs13181 and rs1799793, ERCC4 rs1800067, and ERCC5 rs17655 gene polymorphisms were assessed ... more

M.J. Wang; Y. Zhu; X.J. Guo; Z.Z. Tian
Chemotherapy; ERCC1; ERCC2; Osteosarcoma; Overall survival; Single nucleotide polymorphism

We assessed the role of single nucleotide polymorphisms (SNPs) in ERCC1 and ERCC2 genes in the clinical outcomes for osteosarcoma patients receiving cisplatin-based treatment. A perspective study was conducted on 260 patients with osteosarcoma during 2010 and 2011. A polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was used to assess ... more

Q. Zhang; L.Y. Lv; B.J. Li; J. Zhang; F. Wei
Osteosarcoma; Polymorphisms; VEGF

The aim of this study was to assess the role of the VEGF -2578C/A, +936C/T, and -460T/C gene polymorphisms in the development of osteosarcoma. A total of 182 patients with osteosarcoma and 182 age- and gender-matched healthy controls were enrolled into our study during January 2011 and December 2013. Genotype frequencies of the VEGF -2578C/A and -460T/C alleles in controls ... more

H.F. Zhang; J.P. Yan; Y.S. Zhuang; G.Q. Han