Research Article

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10/28/2008
Endangered animals; Mitochondrial DNA; Oryx species; Phylogeny; rRNA genes

We conducted a comparative evaluation of 12S rRNA and 16S rRNA genes of the mitochondrial genome for molecular differentiation among three oryx species (Oryx leucoryx, Oryx dammah and Oryx gazella) with respect to two closely related outgroups, addax and roan. Our findings showed the failure of 12S rRNA gene to differentiate between the genus Oryx and addax ... more

H.A. Khan; I.A. Arif; A.H.Al Farhan; A.A.Al Homaidan
07/01/2008
Bos indicus; Bos taurus; Guzerat; Mitochondrial DNA; production; Reproduction

The observation of bovine mitochondrial DNA (mtDNA) polymorphisms allows the separation of American zebu cattle, according to its maternal lineage ancestry, into two groups: one with Bos indicus mtDNA and other with Bos taurus mtDNA. The aim of the present study was to determine the productive and reproductive differences between these two groups, in a Guzerat dairy herd. The ... more

J.C.C. Paneto; J.B.S. Ferraz; J.C.C. Balieiro; J.F.F. Bittar; M.B.D. Ferreira; M.B. Leite; G.K.F. Merighe; F.V. Meirelles
12/21/2011
Cytochrome C oxidase 1; Mitochondrial DNA; Molecular phylogeny

Morphology and biogeography are widely used in animal taxonomy. Recent study has suggested that a DNA-based identification system, using a 648-bp portion of the mitochondrial gene cytochrome oxidase subunit 1 (CO1), also known as the barcoding gene, can aid in the resolution of inferences concerning phylogenetic relationships and for identification of species. However, ... more

D.B. Yu; R. Chen; H.A. Kaleri; B.C. Jiang; H.X. Xu; W.X. Du
10/31/2011
Hucul horse; Mitochondrial DNA; Phylogeny; Primitive

The existence of the Hucul horse on Romanian territory has been documented from the very distant past; today Hucul is a unique breed that is part of the FAO Program for the Preservation of Animal Genetic Resources. We compared Hucul with several primitive European and Asiatic breeds in order to elucidate the origin of these horses. We analyzed a 683-bp mitochondrial DNA (mtDNA) D-loop ... more

S.E. Georgescu; M.A. Manea; A. Dudu; M. Costache
11/09/2011
Dispersal; Mitochondrial DNA; Onychophora; Population expansion; Population structure

Epiperipatus acacioi (Onychophora: Peripatidae) is an endemic species of the Atlantic rainforest in southeastern Brazil, with a restricted known distribution, found only in two nearby areas (Tripuí and Itacolomi). Mitochondrial gene COI sequences of 93 specimens collected across the known range of E. acacioi were used to assess the extant genetic diversity and patterns ... more

G.A. Lacorte; I.S. Oliveira; C.G. Fonseca
12/09/2013
Artificial reproduction; Black rockfish; Genetic variability; Microsatellite loci; Mitochondrial DNA; Sebastes inermis

The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a mitochondrial (mt) ... more

H.S. An; J.W. Lee; J.Y. Park; J.I. Myeong; C.M. An
09/25/2012
Malaria; Mitochondrial DNA; Plasmodium falciparum; Plasmodium vivax; Real-time PCR

The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probes. Since the first study published ... more

C.R.T. Souza; T.A.A. Carvalho; R.C.G. Amaral; L.S. Cunha; M.G. Cunha; J.F. Guerreiro
08/06/2012
Aplastic anemia; maternal inheritance; Mitochondrial DNA; Mutation

This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients’ oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by PCR after extraction, and these segments ... more

X. Cui; F. Liu; J.Q. Wang; W.J. Zhang; J.Y. Wang; K. Liu; S.Y. Cui; J. Zhang; R.R. Xu
12/04/2014
Extraction method; Mitochondrial DNA; Molecular markers; Triticum aestivum

We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from yel­low etiolated shoots of wheat crop (Triticum aestivum). mtDNA ex­traction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applications such as DNA ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; N. Na; Z. Huiyan
02/21/2014
Barker frog; Microsatellite; Mitochondrial DNA; Physalaemus; Population structure

The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated ... more

M. Conte; C.P. Targueta; M.I. Zucchi; A.P. Souza; S.M. Recco-Pimentel

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