Research Article

Related GMR Articles

04/25/2016
Gene expression; Habitual abortion; MCP2; TLR4

Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression in peripheral blood and placental villi of patients and healthy ... more

X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang; X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang; X.P. Li; L.N. Song; L.P. Tian; Y.S. Zhang
04/25/2016
Exopalaemon carinicauda; Gene expression; Internal control; Ovarian development; β-actin

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long ... more

J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie; J.P. Liang; Y. Wang; Q.Q. Ge; J.T. Li; P. Liu; J. Li; G.X. Nie
04/04/2016
Gene expression; Hu sheep; Muscle fiber development; MyHCs; YAP1

RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months old) of Hu Sheep. The results showed that ... more

W. Gao; W. Sun; R. Su; X.Y. Lv; Q.Z. Wang; D. Li; H.H. Musa; L. Chen; H. Zhou; H.S. Xu; W.H. Hua; W. Gao; W. Sun; R. Su; X.Y. Lv; Q.Z. Wang; D. Li; H.H. Musa; L. Chen; H. Zhou; H.S. Xu; W.H. Hua; W. Gao; W. Sun; R. Su; X.Y. Lv; Q.Z. Wang; D. Li; H.H. Musa; L. Chen; H. Zhou; H.S. Xu; W.H. Hua
06/30/2016
avTOR; Chickens; Feed efficiency; Gene expression

Avian target of rapamycin (avTOR) is a highly conserved serine-threonine kinase that serves as an intracellular energy and nutrient sensor and regulates cell division, growth, and apoptosis. The role of avTOR in mediating feed intake and growth in poultry is unknown. We studied avTOR signaling activities in duodenum and liver tissues at days 35 and 42 in chickens divergently selected for ... more

J. Lee; S.E. Aggrey; J. Lee; S.E. Aggrey
06/17/2016
Gene expression; IGF1R; Pyometra; Real-time quantitative PCR; Reference genes

Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by quantitative measurements of mRNA levels ... more

M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma; M. Du; X. Wang; Y.W. Yue; P.Y. Zhou; W. Yao; X. Li; X.B. Ding; X.F. Liu; H. Guo; W.Z. Ma
05/25/2016
Electron transport chain; Energy metabolism; Gene expression; Oxidative phosphorylation

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was ... more

S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira; S.C.C. Silva; E. Gasparino; E. Batista; F. Tanamati; A.P.D. Vesco; B. Lala; D.P. de Oliveira
05/23/2016
Gene expression; Hu sheep; MSTN; MyoG; Smads

The mRNA expression levels of key genes (Smads, MSTN, and MyoG) in the TGF-β/Smad signaling pathway in Hu sheep at different growth stages (2 days, 2 months, and 6 months of age) and in different skeletal muscles (longissimus dorsi muscle and soleus muscle) and different genders were detected; and correlation of the Smad family (Smad2 ... more

Q.Z. Wang; R. Su; X.Y. Lv; W. Gao; L. Chen; J.J. Bao; J.R. Yu; L.H. Wang; W. Sun; Q.Z. Wang; R. Su; X.Y. Lv; W. Gao; L. Chen; J.J. Bao; J.R. Yu; L.H. Wang; W. Sun
05/23/2016
CD36; Gene expression; Genetic variation; Serum biochemical indices

Cluster of differentiation 36 (CD36) plays a crucial role in lipid sensing, innate immunity, atherogenesis, and glycolipid metabolism. This aims of this study were to delineate the CD36 mRNA expression profile in 16 duck tissues using relative quantitative real-time PCR and to screen single nucleotide polymorphisms (SNPs) in the duck CD36 gene by PCR-single strand ... more

D.D. Wang; W.G. Li; Y.Y. Zhang; D.D. Wang; W.G. Li; Y.Y. Zhang
12/02/2016
Animals; Avian Proteins; Body weight; Breeding; Chickens; Female; Gene expression; Gene Expression Regulation; male; Muscle development; Myostatin; Organ Specificity; Phenotype; Quantitative trait loci

Myostatin (MSTN) is expressed in the myotome and developing skeletal muscles, and acts to regulate the number of muscle fibers. Wuding chicken large body, developed muscle, high disease resistance, and tender, delicious meat, and are not selected for fast growth. Broiler chickens (Avian broiler) are selected for fast growth and have a large body size and high muscle mass. Here, 240 one-day-old ... more

L.X. Liu; T.F. Dou; Q.H. Li; H. Rong; H.Q. Tong; Z.Q. Xu; Y. Huang; D.H. Gu; X.B. Chen; C.R. Ge; J.J. Jia
3/16/2017
Animals; Aquaculture; Cichlids; Ethanol; Fish Proteins; Gene Expression Regulation; male; Muscle, Skeletal; Myostatin; Organ Size; Plant Extracts; Propolis

Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed males (GIFT strain) with an ... more

E.L. Buck; I.Y. Mizubuti; A.A. Alfieri; R.A.A. Otonel; L.Y. Buck; F.P. Souza; O.P. Prado-Calixto; A.R. Poveda-Parra; A. Filho; N.M. Lopera-Barrero

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